Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage

CRISPR-associated nucleases are powerful tools for precise genome editing of model systems, including human organoids. Current methods describing fluorescent gene tagging in organoids rely on the generation of DNA double-strand breaks (DSBs) to stimulate homology-directed repair (HDR) or non-homolog...

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Detalles Bibliográficos
Autores principales: Bollen, Yannik, Hageman, Joris H., van Leenen, Petra, Derks, Lucca L. M., Ponsioen, Bas, Buissant des Amorie, Julian R., Verlaan-Klink, Ingrid, van den Bos, Myrna, Terstappen, Leon W. M. M., van Boxtel, Ruben, Snippert, Hugo J. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Methods and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8827455/
https://www.ncbi.nlm.nih.gov/pubmed/35089911
http://dx.doi.org/10.1371/journal.pbio.3001527

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