Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)

Cryofixation has proven to be the gold standard for efficient preservation of native cell ultrastructure compared to chemical fixation, but this approach is not widely used in fluorescence microscopy owing to implementation challenges. Here, we develop Cryo-ExM, a method that preserves native cellul...

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Autores principales: Laporte, Marine H., Klena, Nikolai, Hamel, Virginie, Guichard, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8828483/
https://www.ncbi.nlm.nih.gov/pubmed/35027766
http://dx.doi.org/10.1038/s41592-021-01356-4
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author Laporte, Marine H.
Klena, Nikolai
Hamel, Virginie
Guichard, Paul
author_facet Laporte, Marine H.
Klena, Nikolai
Hamel, Virginie
Guichard, Paul
author_sort Laporte, Marine H.
collection PubMed
description Cryofixation has proven to be the gold standard for efficient preservation of native cell ultrastructure compared to chemical fixation, but this approach is not widely used in fluorescence microscopy owing to implementation challenges. Here, we develop Cryo-ExM, a method that preserves native cellular organization by coupling cryofixation with expansion microscopy. This method bypasses artifacts associated with chemical fixation and its simplicity will contribute to its widespread use in super-resolution microscopy.
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spelling pubmed-88284832022-02-22 Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM) Laporte, Marine H. Klena, Nikolai Hamel, Virginie Guichard, Paul Nat Methods Article Cryofixation has proven to be the gold standard for efficient preservation of native cell ultrastructure compared to chemical fixation, but this approach is not widely used in fluorescence microscopy owing to implementation challenges. Here, we develop Cryo-ExM, a method that preserves native cellular organization by coupling cryofixation with expansion microscopy. This method bypasses artifacts associated with chemical fixation and its simplicity will contribute to its widespread use in super-resolution microscopy. Nature Publishing Group US 2022-01-13 2022 /pmc/articles/PMC8828483/ /pubmed/35027766 http://dx.doi.org/10.1038/s41592-021-01356-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Laporte, Marine H.
Klena, Nikolai
Hamel, Virginie
Guichard, Paul
Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)
title Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)
title_full Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)
title_fullStr Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)
title_full_unstemmed Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)
title_short Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)
title_sort visualizing the native cellular organization by coupling cryofixation with expansion microscopy (cryo-exm)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8828483/
https://www.ncbi.nlm.nih.gov/pubmed/35027766
http://dx.doi.org/10.1038/s41592-021-01356-4
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