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Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)
Cryofixation has proven to be the gold standard for efficient preservation of native cell ultrastructure compared to chemical fixation, but this approach is not widely used in fluorescence microscopy owing to implementation challenges. Here, we develop Cryo-ExM, a method that preserves native cellul...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group US
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8828483/ https://www.ncbi.nlm.nih.gov/pubmed/35027766 http://dx.doi.org/10.1038/s41592-021-01356-4 |