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Ultrasound does not activate but can inhibit in vivo mammalian nerves across a wide range of parameters

Ultrasound (US) has been shown to stimulate brain circuits, however, the ability to excite peripheral nerves with US remains controversial. To the best of our knowledge, there is still no in vivo neural recording study that has applied US stimulation to a nerve isolated from surrounding tissue to co...

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Autores principales: Guo, Hongsun, Offutt, Sarah J., Hamilton II, Mark, Kim, Yohan, Gloeckner, Cory D., Zachs, Daniel P., Alford, Jamu K., Lim, Hubert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8828880/
https://www.ncbi.nlm.nih.gov/pubmed/35140238
http://dx.doi.org/10.1038/s41598-022-05226-7
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author Guo, Hongsun
Offutt, Sarah J.
Hamilton II, Mark
Kim, Yohan
Gloeckner, Cory D.
Zachs, Daniel P.
Alford, Jamu K.
Lim, Hubert H.
author_facet Guo, Hongsun
Offutt, Sarah J.
Hamilton II, Mark
Kim, Yohan
Gloeckner, Cory D.
Zachs, Daniel P.
Alford, Jamu K.
Lim, Hubert H.
author_sort Guo, Hongsun
collection PubMed
description Ultrasound (US) has been shown to stimulate brain circuits, however, the ability to excite peripheral nerves with US remains controversial. To the best of our knowledge, there is still no in vivo neural recording study that has applied US stimulation to a nerve isolated from surrounding tissue to confirm direct activation effects. Here, we show that US cannot excite an isolated mammalian sciatic nerve in an in vivo preparation, even at high pressures (relative to levels recommended in the FDA guidance for diagnostic ultrasound) and for a wide range of parameters, including different pulse patterns and center frequencies. US can, however, reliably inhibit nerve activity whereby greater suppression is correlated with increases in nerve temperature. By prohibiting the nerve temperature from increasing during US application, we did not observe suppressive effects. Overall, these findings demonstrate that US can reliably inhibit nerve activity through a thermal mechanism that has potential for various health disorders, though future studies are needed to evaluate the long-term safety of therapeutic ultrasound applications.
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spelling pubmed-88288802022-02-14 Ultrasound does not activate but can inhibit in vivo mammalian nerves across a wide range of parameters Guo, Hongsun Offutt, Sarah J. Hamilton II, Mark Kim, Yohan Gloeckner, Cory D. Zachs, Daniel P. Alford, Jamu K. Lim, Hubert H. Sci Rep Article Ultrasound (US) has been shown to stimulate brain circuits, however, the ability to excite peripheral nerves with US remains controversial. To the best of our knowledge, there is still no in vivo neural recording study that has applied US stimulation to a nerve isolated from surrounding tissue to confirm direct activation effects. Here, we show that US cannot excite an isolated mammalian sciatic nerve in an in vivo preparation, even at high pressures (relative to levels recommended in the FDA guidance for diagnostic ultrasound) and for a wide range of parameters, including different pulse patterns and center frequencies. US can, however, reliably inhibit nerve activity whereby greater suppression is correlated with increases in nerve temperature. By prohibiting the nerve temperature from increasing during US application, we did not observe suppressive effects. Overall, these findings demonstrate that US can reliably inhibit nerve activity through a thermal mechanism that has potential for various health disorders, though future studies are needed to evaluate the long-term safety of therapeutic ultrasound applications. Nature Publishing Group UK 2022-02-09 /pmc/articles/PMC8828880/ /pubmed/35140238 http://dx.doi.org/10.1038/s41598-022-05226-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Guo, Hongsun
Offutt, Sarah J.
Hamilton II, Mark
Kim, Yohan
Gloeckner, Cory D.
Zachs, Daniel P.
Alford, Jamu K.
Lim, Hubert H.
Ultrasound does not activate but can inhibit in vivo mammalian nerves across a wide range of parameters
title Ultrasound does not activate but can inhibit in vivo mammalian nerves across a wide range of parameters
title_full Ultrasound does not activate but can inhibit in vivo mammalian nerves across a wide range of parameters
title_fullStr Ultrasound does not activate but can inhibit in vivo mammalian nerves across a wide range of parameters
title_full_unstemmed Ultrasound does not activate but can inhibit in vivo mammalian nerves across a wide range of parameters
title_short Ultrasound does not activate but can inhibit in vivo mammalian nerves across a wide range of parameters
title_sort ultrasound does not activate but can inhibit in vivo mammalian nerves across a wide range of parameters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8828880/
https://www.ncbi.nlm.nih.gov/pubmed/35140238
http://dx.doi.org/10.1038/s41598-022-05226-7
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