Cargando…

Comparative Genomics of Three Colletotrichum scovillei Strains and Genetic Analysis Revealed Genes Involved in Fungal Growth and Virulence on Chili Pepper

Colletotrichum scovillei causes anthracnose of chili pepper in many countries. Three strains of this pathogen, Coll-524, Coll-153, and Coll-365, show varied virulence on chili pepper. Among the three strains, Coll-365 showed significant defects in growth and virulence. To decipher the genetic variat...

Descripción completa

Detalles Bibliográficos
Autores principales: Hsieh, Dai-Keng, Chuang, Shu-Cheng, Chen, Chun-Yi, Chao, Ya-Ting, Lu, Mei-Yeh Jade, Lee, Miin-Huey, Shih, Ming-Che
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8828978/
https://www.ncbi.nlm.nih.gov/pubmed/35154058
http://dx.doi.org/10.3389/fmicb.2022.818291
_version_ 1784647964106424320
author Hsieh, Dai-Keng
Chuang, Shu-Cheng
Chen, Chun-Yi
Chao, Ya-Ting
Lu, Mei-Yeh Jade
Lee, Miin-Huey
Shih, Ming-Che
author_facet Hsieh, Dai-Keng
Chuang, Shu-Cheng
Chen, Chun-Yi
Chao, Ya-Ting
Lu, Mei-Yeh Jade
Lee, Miin-Huey
Shih, Ming-Che
author_sort Hsieh, Dai-Keng
collection PubMed
description Colletotrichum scovillei causes anthracnose of chili pepper in many countries. Three strains of this pathogen, Coll-524, Coll-153, and Coll-365, show varied virulence on chili pepper. Among the three strains, Coll-365 showed significant defects in growth and virulence. To decipher the genetic variations among these strains and identify genes contributing to growth and virulence, comparative genomic analysis and gene transformation to show gene function were applied in this study. Compared to Coll-524, Coll-153, and Coll-365 had numerous gene losses including 32 candidate effector genes that are mainly exist in acutatum species complex. A cluster of 14 genes in a 34-kb genomic fragment was lost in Coll-365. Through gene transformation, three genes in the 34-kb fragment were identified to have functions in growth and/or virulence of C. scovillei. CsPLAA encoding a phospholipase A2-activating protein enhanced the growth of Coll-365. A combination of CsPLAA with one transcription factor CsBZTF and one C6 zinc finger domain-containing protein CsCZCP was found to enhance the pathogenicity of Coll-365. Introduction of CsGIP, which encodes a hypothetical protein, into Coll-365 caused a reduction in the germination rate of Coll-365. In conclusion, the highest virulent strain Coll-524 had more genes and encoded more pathogenicity related proteins and transposable elements than the other two strains, which may contribute to the high virulence of Coll-524. In addition, the absence of the 34-kb fragment plays a critical role in the defects of growth and virulence of strain Coll-365.
format Online
Article
Text
id pubmed-8828978
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88289782022-02-11 Comparative Genomics of Three Colletotrichum scovillei Strains and Genetic Analysis Revealed Genes Involved in Fungal Growth and Virulence on Chili Pepper Hsieh, Dai-Keng Chuang, Shu-Cheng Chen, Chun-Yi Chao, Ya-Ting Lu, Mei-Yeh Jade Lee, Miin-Huey Shih, Ming-Che Front Microbiol Microbiology Colletotrichum scovillei causes anthracnose of chili pepper in many countries. Three strains of this pathogen, Coll-524, Coll-153, and Coll-365, show varied virulence on chili pepper. Among the three strains, Coll-365 showed significant defects in growth and virulence. To decipher the genetic variations among these strains and identify genes contributing to growth and virulence, comparative genomic analysis and gene transformation to show gene function were applied in this study. Compared to Coll-524, Coll-153, and Coll-365 had numerous gene losses including 32 candidate effector genes that are mainly exist in acutatum species complex. A cluster of 14 genes in a 34-kb genomic fragment was lost in Coll-365. Through gene transformation, three genes in the 34-kb fragment were identified to have functions in growth and/or virulence of C. scovillei. CsPLAA encoding a phospholipase A2-activating protein enhanced the growth of Coll-365. A combination of CsPLAA with one transcription factor CsBZTF and one C6 zinc finger domain-containing protein CsCZCP was found to enhance the pathogenicity of Coll-365. Introduction of CsGIP, which encodes a hypothetical protein, into Coll-365 caused a reduction in the germination rate of Coll-365. In conclusion, the highest virulent strain Coll-524 had more genes and encoded more pathogenicity related proteins and transposable elements than the other two strains, which may contribute to the high virulence of Coll-524. In addition, the absence of the 34-kb fragment plays a critical role in the defects of growth and virulence of strain Coll-365. Frontiers Media S.A. 2022-01-27 /pmc/articles/PMC8828978/ /pubmed/35154058 http://dx.doi.org/10.3389/fmicb.2022.818291 Text en Copyright © 2022 Hsieh, Chuang, Chen, Chao, Lu, Lee and Shih. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hsieh, Dai-Keng
Chuang, Shu-Cheng
Chen, Chun-Yi
Chao, Ya-Ting
Lu, Mei-Yeh Jade
Lee, Miin-Huey
Shih, Ming-Che
Comparative Genomics of Three Colletotrichum scovillei Strains and Genetic Analysis Revealed Genes Involved in Fungal Growth and Virulence on Chili Pepper
title Comparative Genomics of Three Colletotrichum scovillei Strains and Genetic Analysis Revealed Genes Involved in Fungal Growth and Virulence on Chili Pepper
title_full Comparative Genomics of Three Colletotrichum scovillei Strains and Genetic Analysis Revealed Genes Involved in Fungal Growth and Virulence on Chili Pepper
title_fullStr Comparative Genomics of Three Colletotrichum scovillei Strains and Genetic Analysis Revealed Genes Involved in Fungal Growth and Virulence on Chili Pepper
title_full_unstemmed Comparative Genomics of Three Colletotrichum scovillei Strains and Genetic Analysis Revealed Genes Involved in Fungal Growth and Virulence on Chili Pepper
title_short Comparative Genomics of Three Colletotrichum scovillei Strains and Genetic Analysis Revealed Genes Involved in Fungal Growth and Virulence on Chili Pepper
title_sort comparative genomics of three colletotrichum scovillei strains and genetic analysis revealed genes involved in fungal growth and virulence on chili pepper
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8828978/
https://www.ncbi.nlm.nih.gov/pubmed/35154058
http://dx.doi.org/10.3389/fmicb.2022.818291
work_keys_str_mv AT hsiehdaikeng comparativegenomicsofthreecolletotrichumscovilleistrainsandgeneticanalysisrevealedgenesinvolvedinfungalgrowthandvirulenceonchilipepper
AT chuangshucheng comparativegenomicsofthreecolletotrichumscovilleistrainsandgeneticanalysisrevealedgenesinvolvedinfungalgrowthandvirulenceonchilipepper
AT chenchunyi comparativegenomicsofthreecolletotrichumscovilleistrainsandgeneticanalysisrevealedgenesinvolvedinfungalgrowthandvirulenceonchilipepper
AT chaoyating comparativegenomicsofthreecolletotrichumscovilleistrainsandgeneticanalysisrevealedgenesinvolvedinfungalgrowthandvirulenceonchilipepper
AT lumeiyehjade comparativegenomicsofthreecolletotrichumscovilleistrainsandgeneticanalysisrevealedgenesinvolvedinfungalgrowthandvirulenceonchilipepper
AT leemiinhuey comparativegenomicsofthreecolletotrichumscovilleistrainsandgeneticanalysisrevealedgenesinvolvedinfungalgrowthandvirulenceonchilipepper
AT shihmingche comparativegenomicsofthreecolletotrichumscovilleistrainsandgeneticanalysisrevealedgenesinvolvedinfungalgrowthandvirulenceonchilipepper