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The Validation of a Single Multiplex Typing System With 45 Y-STR Markers for Familial Searching and Database Construction

The Y-chromosomal short tandem repeat (Y-STR) is an effective forensic tool in familial searches and patrilineal relationship evaluation. However, currently available Y-STR panels often lack sufficient discriminatory power to resolve genetic relationships between distant relatives or within patriloc...

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Autores principales: Zeng, Ying, Chen, Ling, Wang, Mengge, Yang, Chengliang, Liu, Hong, Xiao, Cheng, Liu, ChangHui, Li, Yue, Xu, Quyi, Du, Weian, Liu, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8829124/
https://www.ncbi.nlm.nih.gov/pubmed/35154290
http://dx.doi.org/10.3389/fgene.2022.842004
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author Zeng, Ying
Chen, Ling
Wang, Mengge
Yang, Chengliang
Liu, Hong
Xiao, Cheng
Liu, ChangHui
Li, Yue
Xu, Quyi
Du, Weian
Liu, Chao
author_facet Zeng, Ying
Chen, Ling
Wang, Mengge
Yang, Chengliang
Liu, Hong
Xiao, Cheng
Liu, ChangHui
Li, Yue
Xu, Quyi
Du, Weian
Liu, Chao
author_sort Zeng, Ying
collection PubMed
description The Y-chromosomal short tandem repeat (Y-STR) is an effective forensic tool in familial searches and patrilineal relationship evaluation. However, currently available Y-STR panels often lack sufficient discriminatory power to resolve genetic relationships between distant relatives or within patrilocal populations. This study aims to establish a novel Y-STR amplification system for forensic casework analysis and database construction, which contains 44 slowly and moderately mutating and one rapidly mutating Y-STR. The validation of the assay was conducted following the recommendations of SWGDAM developmental validation guidelines. Different types of casework samples were tested and reliable profiles were obtained. Furthermore, we genotyped and analyzed 141 unrelated Han Chinese male samples. The results showed that this Y45 kit could improve the performance of identifying male individuals, higher haplotype diversity, and discrimination capacity when compared to the previous widely used Yfiler Plus kit. In general, the validation study demonstrated that the newly developed Y45 kit possesses high sensitivity, inhibitor tolerance, male specificity in a mixture, species specificity, and precision and is capable of forensic casework analysis and database construction.
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spelling pubmed-88291242022-02-11 The Validation of a Single Multiplex Typing System With 45 Y-STR Markers for Familial Searching and Database Construction Zeng, Ying Chen, Ling Wang, Mengge Yang, Chengliang Liu, Hong Xiao, Cheng Liu, ChangHui Li, Yue Xu, Quyi Du, Weian Liu, Chao Front Genet Genetics The Y-chromosomal short tandem repeat (Y-STR) is an effective forensic tool in familial searches and patrilineal relationship evaluation. However, currently available Y-STR panels often lack sufficient discriminatory power to resolve genetic relationships between distant relatives or within patrilocal populations. This study aims to establish a novel Y-STR amplification system for forensic casework analysis and database construction, which contains 44 slowly and moderately mutating and one rapidly mutating Y-STR. The validation of the assay was conducted following the recommendations of SWGDAM developmental validation guidelines. Different types of casework samples were tested and reliable profiles were obtained. Furthermore, we genotyped and analyzed 141 unrelated Han Chinese male samples. The results showed that this Y45 kit could improve the performance of identifying male individuals, higher haplotype diversity, and discrimination capacity when compared to the previous widely used Yfiler Plus kit. In general, the validation study demonstrated that the newly developed Y45 kit possesses high sensitivity, inhibitor tolerance, male specificity in a mixture, species specificity, and precision and is capable of forensic casework analysis and database construction. Frontiers Media S.A. 2022-01-27 /pmc/articles/PMC8829124/ /pubmed/35154290 http://dx.doi.org/10.3389/fgene.2022.842004 Text en Copyright © 2022 Zeng, Chen, Wang, Yang, Liu, Xiao, Liu, Li, Xu, Du and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zeng, Ying
Chen, Ling
Wang, Mengge
Yang, Chengliang
Liu, Hong
Xiao, Cheng
Liu, ChangHui
Li, Yue
Xu, Quyi
Du, Weian
Liu, Chao
The Validation of a Single Multiplex Typing System With 45 Y-STR Markers for Familial Searching and Database Construction
title The Validation of a Single Multiplex Typing System With 45 Y-STR Markers for Familial Searching and Database Construction
title_full The Validation of a Single Multiplex Typing System With 45 Y-STR Markers for Familial Searching and Database Construction
title_fullStr The Validation of a Single Multiplex Typing System With 45 Y-STR Markers for Familial Searching and Database Construction
title_full_unstemmed The Validation of a Single Multiplex Typing System With 45 Y-STR Markers for Familial Searching and Database Construction
title_short The Validation of a Single Multiplex Typing System With 45 Y-STR Markers for Familial Searching and Database Construction
title_sort validation of a single multiplex typing system with 45 y-str markers for familial searching and database construction
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8829124/
https://www.ncbi.nlm.nih.gov/pubmed/35154290
http://dx.doi.org/10.3389/fgene.2022.842004
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