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Architecture and Dynamics of the Wounding-Induced Gene Regulatory Network During the Oolong Tea Manufacturing Process (Camellia sinensis)

Understanding extensive transcriptional reprogramming events mediated by wounding during the oolong tea manufacturing process is essential for improving oolong tea quality. To improve our comprehension of the architecture of the wounding-induced gene regulatory network, we systematically analyzed th...

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Autores principales: Zheng, Yucheng, Hu, Qingcai, Yang, Yun, Wu, Zongjie, Wu, Liangyu, Wang, Pengjie, Deng, Huili, Ye, Naixing, Sun, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8829136/
https://www.ncbi.nlm.nih.gov/pubmed/35154182
http://dx.doi.org/10.3389/fpls.2021.788469
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author Zheng, Yucheng
Hu, Qingcai
Yang, Yun
Wu, Zongjie
Wu, Liangyu
Wang, Pengjie
Deng, Huili
Ye, Naixing
Sun, Yun
author_facet Zheng, Yucheng
Hu, Qingcai
Yang, Yun
Wu, Zongjie
Wu, Liangyu
Wang, Pengjie
Deng, Huili
Ye, Naixing
Sun, Yun
author_sort Zheng, Yucheng
collection PubMed
description Understanding extensive transcriptional reprogramming events mediated by wounding during the oolong tea manufacturing process is essential for improving oolong tea quality. To improve our comprehension of the architecture of the wounding-induced gene regulatory network, we systematically analyzed the high-resolution transcriptomic and metabolomic data from wounding-treated (after turnover stage) tea leaves at 11 time points over a 220-min period. The results indicated that wounding activates a burst of transcriptional activity within 10 min and that the temporal expression patterns over time could be partitioned into 18 specific clusters with distinct biological processes. The transcription factor (TF) activity linked to the TF binding motif participated in specific biological processes within different clusters. A chronological model of the wounding-induced gene regulatory network provides insight into the dynamic transcriptional regulation event after wounding treatment (the turnover stage). Time series data of wounding-induced volatiles reveal the scientific significance of resting for a while after wounding treatment during the actual manufacturing process of oolong tea. Integrating information-rich expression data with information on volatiles allowed us to identify many high-confidence TFs participating in aroma formation regulation after wounding treatment by using weighted gene co-expression network analysis (WGCNA). Collectively, our research revealed the complexity of the wounding-induced gene regulatory network and described wounding-mediated dynamic transcriptional reprogramming events, serving as a valuable theoretical basis for the quality formation of oolong tea during the post-harvest manufacturing process.
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spelling pubmed-88291362022-02-11 Architecture and Dynamics of the Wounding-Induced Gene Regulatory Network During the Oolong Tea Manufacturing Process (Camellia sinensis) Zheng, Yucheng Hu, Qingcai Yang, Yun Wu, Zongjie Wu, Liangyu Wang, Pengjie Deng, Huili Ye, Naixing Sun, Yun Front Plant Sci Plant Science Understanding extensive transcriptional reprogramming events mediated by wounding during the oolong tea manufacturing process is essential for improving oolong tea quality. To improve our comprehension of the architecture of the wounding-induced gene regulatory network, we systematically analyzed the high-resolution transcriptomic and metabolomic data from wounding-treated (after turnover stage) tea leaves at 11 time points over a 220-min period. The results indicated that wounding activates a burst of transcriptional activity within 10 min and that the temporal expression patterns over time could be partitioned into 18 specific clusters with distinct biological processes. The transcription factor (TF) activity linked to the TF binding motif participated in specific biological processes within different clusters. A chronological model of the wounding-induced gene regulatory network provides insight into the dynamic transcriptional regulation event after wounding treatment (the turnover stage). Time series data of wounding-induced volatiles reveal the scientific significance of resting for a while after wounding treatment during the actual manufacturing process of oolong tea. Integrating information-rich expression data with information on volatiles allowed us to identify many high-confidence TFs participating in aroma formation regulation after wounding treatment by using weighted gene co-expression network analysis (WGCNA). Collectively, our research revealed the complexity of the wounding-induced gene regulatory network and described wounding-mediated dynamic transcriptional reprogramming events, serving as a valuable theoretical basis for the quality formation of oolong tea during the post-harvest manufacturing process. Frontiers Media S.A. 2022-01-27 /pmc/articles/PMC8829136/ /pubmed/35154182 http://dx.doi.org/10.3389/fpls.2021.788469 Text en Copyright © 2022 Zheng, Hu, Yang, Wu, Wu, Wang, Deng, Ye and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zheng, Yucheng
Hu, Qingcai
Yang, Yun
Wu, Zongjie
Wu, Liangyu
Wang, Pengjie
Deng, Huili
Ye, Naixing
Sun, Yun
Architecture and Dynamics of the Wounding-Induced Gene Regulatory Network During the Oolong Tea Manufacturing Process (Camellia sinensis)
title Architecture and Dynamics of the Wounding-Induced Gene Regulatory Network During the Oolong Tea Manufacturing Process (Camellia sinensis)
title_full Architecture and Dynamics of the Wounding-Induced Gene Regulatory Network During the Oolong Tea Manufacturing Process (Camellia sinensis)
title_fullStr Architecture and Dynamics of the Wounding-Induced Gene Regulatory Network During the Oolong Tea Manufacturing Process (Camellia sinensis)
title_full_unstemmed Architecture and Dynamics of the Wounding-Induced Gene Regulatory Network During the Oolong Tea Manufacturing Process (Camellia sinensis)
title_short Architecture and Dynamics of the Wounding-Induced Gene Regulatory Network During the Oolong Tea Manufacturing Process (Camellia sinensis)
title_sort architecture and dynamics of the wounding-induced gene regulatory network during the oolong tea manufacturing process (camellia sinensis)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8829136/
https://www.ncbi.nlm.nih.gov/pubmed/35154182
http://dx.doi.org/10.3389/fpls.2021.788469
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