Apigenin induces oxidative stress in mouse Sertoli TM4 cells

BACKGROUND AND AIM: Apigenin (API) is an estrogenic compound found in many plants. Sertoli cells reside in the testis and are a key target of environmental toxicants. This study aimed to examine the cytotoxicity, especially oxidative stress of API in mouse Sertoli TM4 cells. MATERIALS AND METHODS: M...

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Autores principales: Jittapalapong, Sathaporn, Poompoung, Thapanee, Sutjarit, Samak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8829396/
https://www.ncbi.nlm.nih.gov/pubmed/35153403
http://dx.doi.org/10.14202/vetworld.2021.3132-3137
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author Jittapalapong, Sathaporn
Poompoung, Thapanee
Sutjarit, Samak
author_facet Jittapalapong, Sathaporn
Poompoung, Thapanee
Sutjarit, Samak
author_sort Jittapalapong, Sathaporn
collection PubMed
description BACKGROUND AND AIM: Apigenin (API) is an estrogenic compound found in many plants. Sertoli cells reside in the testis and are a key target of environmental toxicants. This study aimed to examine the cytotoxicity, especially oxidative stress of API in mouse Sertoli TM4 cells. MATERIALS AND METHODS: Mouse Sertoli TM4 cells were treated with 50 and 100 μM API for 48 h. Cell viability, lactate dehydrogenase (LDH) activities, glutathione reductase (GR) activities, production of reactive oxygen species (ROS), and malondialdehyde (MDA) levels were evaluated using various assays. RESULTS: Treatment with API at both 50 and 100 μM decreased viability and GR activity but increased LDH activity, ROS production, and MDA levels in mouse Sertoli TM4 cells. CONCLUSION: Exposure to API induced oxidative stress in mouse Sertoli TM4 cells.
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spelling pubmed-88293962022-02-12 Apigenin induces oxidative stress in mouse Sertoli TM4 cells Jittapalapong, Sathaporn Poompoung, Thapanee Sutjarit, Samak Vet World Research Article BACKGROUND AND AIM: Apigenin (API) is an estrogenic compound found in many plants. Sertoli cells reside in the testis and are a key target of environmental toxicants. This study aimed to examine the cytotoxicity, especially oxidative stress of API in mouse Sertoli TM4 cells. MATERIALS AND METHODS: Mouse Sertoli TM4 cells were treated with 50 and 100 μM API for 48 h. Cell viability, lactate dehydrogenase (LDH) activities, glutathione reductase (GR) activities, production of reactive oxygen species (ROS), and malondialdehyde (MDA) levels were evaluated using various assays. RESULTS: Treatment with API at both 50 and 100 μM decreased viability and GR activity but increased LDH activity, ROS production, and MDA levels in mouse Sertoli TM4 cells. CONCLUSION: Exposure to API induced oxidative stress in mouse Sertoli TM4 cells. Veterinary World 2021-12 2021-12-20 /pmc/articles/PMC8829396/ /pubmed/35153403 http://dx.doi.org/10.14202/vetworld.2021.3132-3137 Text en Copyright: © Jittapalapong, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Jittapalapong, Sathaporn
Poompoung, Thapanee
Sutjarit, Samak
Apigenin induces oxidative stress in mouse Sertoli TM4 cells
title Apigenin induces oxidative stress in mouse Sertoli TM4 cells
title_full Apigenin induces oxidative stress in mouse Sertoli TM4 cells
title_fullStr Apigenin induces oxidative stress in mouse Sertoli TM4 cells
title_full_unstemmed Apigenin induces oxidative stress in mouse Sertoli TM4 cells
title_short Apigenin induces oxidative stress in mouse Sertoli TM4 cells
title_sort apigenin induces oxidative stress in mouse sertoli tm4 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8829396/
https://www.ncbi.nlm.nih.gov/pubmed/35153403
http://dx.doi.org/10.14202/vetworld.2021.3132-3137
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AT sutjaritsamak apigenininducesoxidativestressinmousesertolitm4cells

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