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Chitosan from Crabs (Scylla serrata) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression

Hepato-renal dysfunctions associated with hyperlipidemia necessitates a continuous search for natural remedies. This study thus evaluated the effect of dietary chitosan on diet-induced hyperlipidemia in rats. A total of 30 male Wistar rats (90 ± 10) g were randomly allotted into six (6) groups (n =...

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Autores principales: Ugbaja, Regina Ngozi, Ogungbemi, Kunle, James, Adewale Segun, Peter Folorunsho, Ayodele, Abolade, Samuel Olanrewaju, Ajamikoko, Stella Onajite, Atayese, Eniola Olapeju, Adedeji, Omowunmi Victoria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8830478/
https://www.ncbi.nlm.nih.gov/pubmed/35366259
http://dx.doi.org/10.3390/pathophysiology28020015
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author Ugbaja, Regina Ngozi
Ogungbemi, Kunle
James, Adewale Segun
Peter Folorunsho, Ayodele
Abolade, Samuel Olanrewaju
Ajamikoko, Stella Onajite
Atayese, Eniola Olapeju
Adedeji, Omowunmi Victoria
author_facet Ugbaja, Regina Ngozi
Ogungbemi, Kunle
James, Adewale Segun
Peter Folorunsho, Ayodele
Abolade, Samuel Olanrewaju
Ajamikoko, Stella Onajite
Atayese, Eniola Olapeju
Adedeji, Omowunmi Victoria
author_sort Ugbaja, Regina Ngozi
collection PubMed
description Hepato-renal dysfunctions associated with hyperlipidemia necessitates a continuous search for natural remedies. This study thus evaluated the effect of dietary chitosan on diet-induced hyperlipidemia in rats. A total of 30 male Wistar rats (90 ± 10) g were randomly allotted into six (6) groups (n = 5): Normal diet, High-fat diet (HFD), and Normal diet + 5% chitosan. The three other groups received HFD, supplemented with 1%, 3%, and 5% of chitosan. The feeding lasted for 6 weeks, after which the rats were sacrificed. The liver and kidneys were harvested for analyses. Hepatic alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activity, and renal biomarkers (ALT, AST, urea, and creatinine) were assayed spectrophotometrically. Additionally, expression of hepatic and renal CD43 and p53 was estimated immunohistochemically. The HFD group had elevated bodyweight compared to normal which was reversed in the chitosan-supplemented groups. Hyperlipidemia caused a significant (p < 0.05) decrease in the hepatic (AST, ALT, and ALP) and renal (AST and ALT) activities, while renal urea and creatinine increased. Furthermore, the HFD group showed an elevated level of hepatic and renal CD43 while p53 expression decreased. However, groups supplemented with chitosan showed improved hepatic and renal biomarkers, as well as corrected the aberrations in the expressions of p53 and CD43. Conclusively, dietary chitosan inclusion in the diet (between 3% and 5%) could effectively improve kidney and liver functionality via abatement of inflammatory responses.
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spelling pubmed-88304782022-03-23 Chitosan from Crabs (Scylla serrata) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression Ugbaja, Regina Ngozi Ogungbemi, Kunle James, Adewale Segun Peter Folorunsho, Ayodele Abolade, Samuel Olanrewaju Ajamikoko, Stella Onajite Atayese, Eniola Olapeju Adedeji, Omowunmi Victoria Pathophysiology Article Hepato-renal dysfunctions associated with hyperlipidemia necessitates a continuous search for natural remedies. This study thus evaluated the effect of dietary chitosan on diet-induced hyperlipidemia in rats. A total of 30 male Wistar rats (90 ± 10) g were randomly allotted into six (6) groups (n = 5): Normal diet, High-fat diet (HFD), and Normal diet + 5% chitosan. The three other groups received HFD, supplemented with 1%, 3%, and 5% of chitosan. The feeding lasted for 6 weeks, after which the rats were sacrificed. The liver and kidneys were harvested for analyses. Hepatic alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activity, and renal biomarkers (ALT, AST, urea, and creatinine) were assayed spectrophotometrically. Additionally, expression of hepatic and renal CD43 and p53 was estimated immunohistochemically. The HFD group had elevated bodyweight compared to normal which was reversed in the chitosan-supplemented groups. Hyperlipidemia caused a significant (p < 0.05) decrease in the hepatic (AST, ALT, and ALP) and renal (AST and ALT) activities, while renal urea and creatinine increased. Furthermore, the HFD group showed an elevated level of hepatic and renal CD43 while p53 expression decreased. However, groups supplemented with chitosan showed improved hepatic and renal biomarkers, as well as corrected the aberrations in the expressions of p53 and CD43. Conclusively, dietary chitosan inclusion in the diet (between 3% and 5%) could effectively improve kidney and liver functionality via abatement of inflammatory responses. MDPI 2021-05-17 /pmc/articles/PMC8830478/ /pubmed/35366259 http://dx.doi.org/10.3390/pathophysiology28020015 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ugbaja, Regina Ngozi
Ogungbemi, Kunle
James, Adewale Segun
Peter Folorunsho, Ayodele
Abolade, Samuel Olanrewaju
Ajamikoko, Stella Onajite
Atayese, Eniola Olapeju
Adedeji, Omowunmi Victoria
Chitosan from Crabs (Scylla serrata) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression
title Chitosan from Crabs (Scylla serrata) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression
title_full Chitosan from Crabs (Scylla serrata) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression
title_fullStr Chitosan from Crabs (Scylla serrata) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression
title_full_unstemmed Chitosan from Crabs (Scylla serrata) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression
title_short Chitosan from Crabs (Scylla serrata) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats: Modulation of CD43 and p53 Expression
title_sort chitosan from crabs (scylla serrata) represses hyperlipidemia-induced hepato-renal dysfunctions in rats: modulation of cd43 and p53 expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8830478/
https://www.ncbi.nlm.nih.gov/pubmed/35366259
http://dx.doi.org/10.3390/pathophysiology28020015
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