Cargando…

Insecticide resistance in Australian Spodoptera frugiperda (J.E. Smith) and development of testing procedures for resistance surveillance

Spodoptera frugiperda (J.E. Smith) is a highly invasive noctuid pest first reported in northern Australia during early 2020. To document current status of resistance in S. frugiperda in Australia, insecticide toxicity was tested in field populations collected during the first year of establishment,...

Descripción completa

Detalles Bibliográficos
Autores principales: Bird, Lisa, Miles, Melina, Quade, Adam, Spafford, Helen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8830740/
https://www.ncbi.nlm.nih.gov/pubmed/35143580
http://dx.doi.org/10.1371/journal.pone.0263677
_version_ 1784648339777650688
author Bird, Lisa
Miles, Melina
Quade, Adam
Spafford, Helen
author_facet Bird, Lisa
Miles, Melina
Quade, Adam
Spafford, Helen
author_sort Bird, Lisa
collection PubMed
description Spodoptera frugiperda (J.E. Smith) is a highly invasive noctuid pest first reported in northern Australia during early 2020. To document current status of resistance in S. frugiperda in Australia, insecticide toxicity was tested in field populations collected during the first year of establishment, between March 2020 and March 2021. Dose-response was measured by larval bioassay in 11 populations of S. frugiperda and a susceptible laboratory strain of Helicoverpa armigera. Emamectin benzoate was the most efficacious insecticide (LC(50) 0.023μg/ml) followed by chlorantraniliprole (LC(50) 0.055μg/ml), spinetoram (LC(50) 0.098μg/ml), spinosad (LC(50) 0.526μg/ml), and methoxyfenozide (1.413μg/ml). Indoxacarb was the least toxic selective insecticide on S. frugiperda (LC(50) 3.789μg/ml). Emamectin benzoate, chlorantraniliprole and methoxyfenozide were 2- to 7-fold less toxic on S. frugiperda compared with H. armigera while spinosyns were equally toxic on both species. Indoxacarb was 28-fold less toxic on S. frugiperda compared with H. armigera. There was decreased sensitivity to Group 1 insecticides and synthetic pyrethroids in S. frugiperda compared with H. armigera: toxicity was reduced up to 11-fold for methomyl, 56 to 199-fold for cyhalothrin, and 44 to 132-fold for alpha cypermethrin. Synergism bioassays with metabolic inhibitors suggest involvement of mixed function oxidase in pyrethroid resistance. Recommended diagnostic doses for emamectin benzoate, chlorantraniliprole, spinetoram, spinosad, methoxyfenozide and indoxacarb are 0.19, 1.0, 0.75, 6, 12 and 48μg/μl, respectively.
format Online
Article
Text
id pubmed-8830740
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-88307402022-02-11 Insecticide resistance in Australian Spodoptera frugiperda (J.E. Smith) and development of testing procedures for resistance surveillance Bird, Lisa Miles, Melina Quade, Adam Spafford, Helen PLoS One Research Article Spodoptera frugiperda (J.E. Smith) is a highly invasive noctuid pest first reported in northern Australia during early 2020. To document current status of resistance in S. frugiperda in Australia, insecticide toxicity was tested in field populations collected during the first year of establishment, between March 2020 and March 2021. Dose-response was measured by larval bioassay in 11 populations of S. frugiperda and a susceptible laboratory strain of Helicoverpa armigera. Emamectin benzoate was the most efficacious insecticide (LC(50) 0.023μg/ml) followed by chlorantraniliprole (LC(50) 0.055μg/ml), spinetoram (LC(50) 0.098μg/ml), spinosad (LC(50) 0.526μg/ml), and methoxyfenozide (1.413μg/ml). Indoxacarb was the least toxic selective insecticide on S. frugiperda (LC(50) 3.789μg/ml). Emamectin benzoate, chlorantraniliprole and methoxyfenozide were 2- to 7-fold less toxic on S. frugiperda compared with H. armigera while spinosyns were equally toxic on both species. Indoxacarb was 28-fold less toxic on S. frugiperda compared with H. armigera. There was decreased sensitivity to Group 1 insecticides and synthetic pyrethroids in S. frugiperda compared with H. armigera: toxicity was reduced up to 11-fold for methomyl, 56 to 199-fold for cyhalothrin, and 44 to 132-fold for alpha cypermethrin. Synergism bioassays with metabolic inhibitors suggest involvement of mixed function oxidase in pyrethroid resistance. Recommended diagnostic doses for emamectin benzoate, chlorantraniliprole, spinetoram, spinosad, methoxyfenozide and indoxacarb are 0.19, 1.0, 0.75, 6, 12 and 48μg/μl, respectively. Public Library of Science 2022-02-10 /pmc/articles/PMC8830740/ /pubmed/35143580 http://dx.doi.org/10.1371/journal.pone.0263677 Text en © 2022 Bird et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bird, Lisa
Miles, Melina
Quade, Adam
Spafford, Helen
Insecticide resistance in Australian Spodoptera frugiperda (J.E. Smith) and development of testing procedures for resistance surveillance
title Insecticide resistance in Australian Spodoptera frugiperda (J.E. Smith) and development of testing procedures for resistance surveillance
title_full Insecticide resistance in Australian Spodoptera frugiperda (J.E. Smith) and development of testing procedures for resistance surveillance
title_fullStr Insecticide resistance in Australian Spodoptera frugiperda (J.E. Smith) and development of testing procedures for resistance surveillance
title_full_unstemmed Insecticide resistance in Australian Spodoptera frugiperda (J.E. Smith) and development of testing procedures for resistance surveillance
title_short Insecticide resistance in Australian Spodoptera frugiperda (J.E. Smith) and development of testing procedures for resistance surveillance
title_sort insecticide resistance in australian spodoptera frugiperda (j.e. smith) and development of testing procedures for resistance surveillance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8830740/
https://www.ncbi.nlm.nih.gov/pubmed/35143580
http://dx.doi.org/10.1371/journal.pone.0263677
work_keys_str_mv AT birdlisa insecticideresistanceinaustralianspodopterafrugiperdajesmithanddevelopmentoftestingproceduresforresistancesurveillance
AT milesmelina insecticideresistanceinaustralianspodopterafrugiperdajesmithanddevelopmentoftestingproceduresforresistancesurveillance
AT quadeadam insecticideresistanceinaustralianspodopterafrugiperdajesmithanddevelopmentoftestingproceduresforresistancesurveillance
AT spaffordhelen insecticideresistanceinaustralianspodopterafrugiperdajesmithanddevelopmentoftestingproceduresforresistancesurveillance