Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures

The LAMP-based eazyplex® BloodScreen GN was evaluated for the detection of frequent Gram-negatives directly from positive blood culture (BC) bottles. A total of 449 BCs were analyzed. Sensitivities and specificities were 100% and 100% for Escherichia coli, 95.7% and 100% for Klebsiella pneumoniae, 1...

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Autores principales: Bach, Katharina, Edel, Birgit, Höring, Steffen, Bartoničkova, Lucie, Glöckner, Stefan, Löffler, Bettina, Bahrs, Christina, Rödel, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8831353/
https://www.ncbi.nlm.nih.gov/pubmed/34807364
http://dx.doi.org/10.1007/s10096-021-04383-3
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author Bach, Katharina
Edel, Birgit
Höring, Steffen
Bartoničkova, Lucie
Glöckner, Stefan
Löffler, Bettina
Bahrs, Christina
Rödel, Jürgen
author_facet Bach, Katharina
Edel, Birgit
Höring, Steffen
Bartoničkova, Lucie
Glöckner, Stefan
Löffler, Bettina
Bahrs, Christina
Rödel, Jürgen
author_sort Bach, Katharina
collection PubMed
description The LAMP-based eazyplex® BloodScreen GN was evaluated for the detection of frequent Gram-negatives directly from positive blood culture (BC) bottles. A total of 449 BCs were analyzed. Sensitivities and specificities were 100% and 100% for Escherichia coli, 95.7% and 100% for Klebsiella pneumoniae, 100% and 100% for bla(CTX-M), 100% and 100% for Klebsiella oxytoca, 100% and 99% for Proteus mirabilis, and 100% and 99.8% for Pseudomonas aeruginosa, respectively. The time to result ranged from 8 to 16 min, plus about 6 min for sample preparation. The eazyplex® BloodScreen GN is a reliable molecular assay for rapid BC testing.
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spelling pubmed-88313532022-02-23 Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures Bach, Katharina Edel, Birgit Höring, Steffen Bartoničkova, Lucie Glöckner, Stefan Löffler, Bettina Bahrs, Christina Rödel, Jürgen Eur J Clin Microbiol Infect Dis Brief Report The LAMP-based eazyplex® BloodScreen GN was evaluated for the detection of frequent Gram-negatives directly from positive blood culture (BC) bottles. A total of 449 BCs were analyzed. Sensitivities and specificities were 100% and 100% for Escherichia coli, 95.7% and 100% for Klebsiella pneumoniae, 100% and 100% for bla(CTX-M), 100% and 100% for Klebsiella oxytoca, 100% and 99% for Proteus mirabilis, and 100% and 99.8% for Pseudomonas aeruginosa, respectively. The time to result ranged from 8 to 16 min, plus about 6 min for sample preparation. The eazyplex® BloodScreen GN is a reliable molecular assay for rapid BC testing. Springer Berlin Heidelberg 2021-11-22 2022 /pmc/articles/PMC8831353/ /pubmed/34807364 http://dx.doi.org/10.1007/s10096-021-04383-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Report
Bach, Katharina
Edel, Birgit
Höring, Steffen
Bartoničkova, Lucie
Glöckner, Stefan
Löffler, Bettina
Bahrs, Christina
Rödel, Jürgen
Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures
title Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures
title_full Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures
title_fullStr Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures
title_full_unstemmed Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures
title_short Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures
title_sort performance of the eazyplex® bloodscreen gn as a simple and rapid molecular test for identification of gram-negative bacteria from positive blood cultures
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8831353/
https://www.ncbi.nlm.nih.gov/pubmed/34807364
http://dx.doi.org/10.1007/s10096-021-04383-3
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