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Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments
With recent advances in microfabrication technologies, the miniaturization of traditional culturing techniques has provided ideal methods for interrogating microbial communities in a confined and finely controlled environment. Micro-technologies offer high-throughput screening and analysis, reduced...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8831547/ https://www.ncbi.nlm.nih.gov/pubmed/35154021 http://dx.doi.org/10.3389/fmicb.2021.745835 |
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author | Davidson, Shanna-Leigh Niepa, Tagbo H. R. |
author_facet | Davidson, Shanna-Leigh Niepa, Tagbo H. R. |
author_sort | Davidson, Shanna-Leigh |
collection | PubMed |
description | With recent advances in microfabrication technologies, the miniaturization of traditional culturing techniques has provided ideal methods for interrogating microbial communities in a confined and finely controlled environment. Micro-technologies offer high-throughput screening and analysis, reduced experimental time and resources, and have low footprint. More importantly, they provide access to culturing microbes in situ in their natural environments and similarly, offer optical access to real-time dynamics under a microscope. Utilizing micro-technologies for the discovery, isolation and cultivation of “unculturable” species will propel many fields forward; drug discovery, point-of-care diagnostics, and fundamental studies in microbial community behaviors rely on the exploration of novel metabolic pathways. However, micro-technologies are still largely proof-of-concept, and scalability and commercialization of micro-technologies will require increased accessibility to expensive equipment and resources, as well as simpler designs for usability. Here, we discuss three different miniaturized culturing practices; including microarrays, micromachined devices, and microfluidics; advancements to the field, and perceived challenges. |
format | Online Article Text |
id | pubmed-8831547 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88315472022-02-12 Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments Davidson, Shanna-Leigh Niepa, Tagbo H. R. Front Microbiol Microbiology With recent advances in microfabrication technologies, the miniaturization of traditional culturing techniques has provided ideal methods for interrogating microbial communities in a confined and finely controlled environment. Micro-technologies offer high-throughput screening and analysis, reduced experimental time and resources, and have low footprint. More importantly, they provide access to culturing microbes in situ in their natural environments and similarly, offer optical access to real-time dynamics under a microscope. Utilizing micro-technologies for the discovery, isolation and cultivation of “unculturable” species will propel many fields forward; drug discovery, point-of-care diagnostics, and fundamental studies in microbial community behaviors rely on the exploration of novel metabolic pathways. However, micro-technologies are still largely proof-of-concept, and scalability and commercialization of micro-technologies will require increased accessibility to expensive equipment and resources, as well as simpler designs for usability. Here, we discuss three different miniaturized culturing practices; including microarrays, micromachined devices, and microfluidics; advancements to the field, and perceived challenges. Frontiers Media S.A. 2022-01-28 /pmc/articles/PMC8831547/ /pubmed/35154021 http://dx.doi.org/10.3389/fmicb.2021.745835 Text en Copyright © 2022 Davidson and Niepa. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Davidson, Shanna-Leigh Niepa, Tagbo H. R. Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments |
title | Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments |
title_full | Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments |
title_fullStr | Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments |
title_full_unstemmed | Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments |
title_short | Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments |
title_sort | micro-technologies for assessing microbial dynamics in controlled environments |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8831547/ https://www.ncbi.nlm.nih.gov/pubmed/35154021 http://dx.doi.org/10.3389/fmicb.2021.745835 |
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