The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2

Cyanobacterial blooming is an increasing environmental issue all over the world. Algicidal bacteria are potential tools for the control of algal blooms. The algicidal activity in many bacteria exhibits quorum-sensing (QS) dynamics and the regulatory mechanism of this activity in these bacteria is un...

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Autores principales: Liu, Jian, Liu, Kaiquan, Zhao, Zhe, Wang, Zheng, Wang, Fengchao, Xin, Yuxiu, Qu, Jie, Song, Feng, Li, Zhenghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8831721/
https://www.ncbi.nlm.nih.gov/pubmed/35154040
http://dx.doi.org/10.3389/fmicb.2021.814929
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author Liu, Jian
Liu, Kaiquan
Zhao, Zhe
Wang, Zheng
Wang, Fengchao
Xin, Yuxiu
Qu, Jie
Song, Feng
Li, Zhenghua
author_facet Liu, Jian
Liu, Kaiquan
Zhao, Zhe
Wang, Zheng
Wang, Fengchao
Xin, Yuxiu
Qu, Jie
Song, Feng
Li, Zhenghua
author_sort Liu, Jian
collection PubMed
description Cyanobacterial blooming is an increasing environmental issue all over the world. Algicidal bacteria are potential tools for the control of algal blooms. The algicidal activity in many bacteria exhibits quorum-sensing (QS) dynamics and the regulatory mechanism of this activity in these bacteria is unclear. In this study, combining genomic sequencing and genome editing, we have identified that the primary quorum-sensing system in the isolated algicidal strain Shewanella xiamenensis Lzh-2 is the LuxS/AI-2 signaling pathway. Disruption of the QS system through recombination deletion of the LuxS gene led to a loss of algicides production and algicidal activity. Restoration of the LuxS gene in the deletion mutant compensated the QS system and recovered the algicidal activity. Consequently, we proved that Lzh-2 regulates the algicidal activity through LuxS/AI-2 quorum-sensing system.
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spelling pubmed-88317212022-02-12 The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2 Liu, Jian Liu, Kaiquan Zhao, Zhe Wang, Zheng Wang, Fengchao Xin, Yuxiu Qu, Jie Song, Feng Li, Zhenghua Front Microbiol Microbiology Cyanobacterial blooming is an increasing environmental issue all over the world. Algicidal bacteria are potential tools for the control of algal blooms. The algicidal activity in many bacteria exhibits quorum-sensing (QS) dynamics and the regulatory mechanism of this activity in these bacteria is unclear. In this study, combining genomic sequencing and genome editing, we have identified that the primary quorum-sensing system in the isolated algicidal strain Shewanella xiamenensis Lzh-2 is the LuxS/AI-2 signaling pathway. Disruption of the QS system through recombination deletion of the LuxS gene led to a loss of algicides production and algicidal activity. Restoration of the LuxS gene in the deletion mutant compensated the QS system and recovered the algicidal activity. Consequently, we proved that Lzh-2 regulates the algicidal activity through LuxS/AI-2 quorum-sensing system. Frontiers Media S.A. 2022-01-28 /pmc/articles/PMC8831721/ /pubmed/35154040 http://dx.doi.org/10.3389/fmicb.2021.814929 Text en Copyright © 2022 Liu, Liu, Zhao, Wang, Wang, Xin, Qu, Song and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Jian
Liu, Kaiquan
Zhao, Zhe
Wang, Zheng
Wang, Fengchao
Xin, Yuxiu
Qu, Jie
Song, Feng
Li, Zhenghua
The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2
title The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2
title_full The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2
title_fullStr The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2
title_full_unstemmed The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2
title_short The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2
title_sort luxs/ai-2 quorum-sensing system regulates the algicidal activity of shewanella xiamenensis lzh-2
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8831721/
https://www.ncbi.nlm.nih.gov/pubmed/35154040
http://dx.doi.org/10.3389/fmicb.2021.814929
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