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Isolation and Genomic Characterization of Avian Reovirus From Wild Birds in South Korea

Avian reoviruses (ARVs) cause severe arthritis, tenosynovitis, pericarditis, and depressed growth in chickens, and these conditions have become increasingly frequent in recent years. Studies on the role of wild birds in the epidemiology of ARVs are insufficient. This study provides information about...

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Autores principales: Kim, Sang-Won, Choi, Yu-Ri, Park, Jong-Yeol, Wei, Bai, Shang, Ke, Zhang, Jun-Feng, Jang, Hyung-Kwan, Cha, Se-Yeoun, Kang, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8831841/
https://www.ncbi.nlm.nih.gov/pubmed/35155656
http://dx.doi.org/10.3389/fvets.2022.794934
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author Kim, Sang-Won
Choi, Yu-Ri
Park, Jong-Yeol
Wei, Bai
Shang, Ke
Zhang, Jun-Feng
Jang, Hyung-Kwan
Cha, Se-Yeoun
Kang, Min
author_facet Kim, Sang-Won
Choi, Yu-Ri
Park, Jong-Yeol
Wei, Bai
Shang, Ke
Zhang, Jun-Feng
Jang, Hyung-Kwan
Cha, Se-Yeoun
Kang, Min
author_sort Kim, Sang-Won
collection PubMed
description Avian reoviruses (ARVs) cause severe arthritis, tenosynovitis, pericarditis, and depressed growth in chickens, and these conditions have become increasingly frequent in recent years. Studies on the role of wild birds in the epidemiology of ARVs are insufficient. This study provides information about currently circulating ARVs in wild birds by gene detection using diagnostic RT-PCR, virus isolation, and genomic characterization. In this study, we isolated and identified 10 ARV isolates from 7,390 wild birds' fecal samples, including migratory bird species (bean goose, Eurasian teal, Indian spot-billed duck, and mallard duck) from 2015 to 2019 in South Korea. On comparing the amino acid sequences of the σC-encoding gene, most isolates, except A18-13, shared higher sequence similarity with the commercial vaccine isolate S1133 and Chinese isolates. However, the A18-13 isolate is similar to live attenuated vaccine av-S1133 and vaccine break isolates (SD09-1, LN09-1, and GX110116). For the p10- and p17-encoding genes, all isolates have identical fusion associated small transmembrane (FAST) protein and nuclear localization signal (SNL) motif to chicken-origin ARVs. Phylogenetic analysis of the amino acid sequences of the σC-encoding gene revealed that all isolates were belonged to genotypic cluster I. For the p10- and p17-encoding genes, the nucleotide sequences of all isolates indicated close relationship with commercial vaccine isolate S1133 and Chinese isolates. For the σNS-encoding gene, the nucleotide sequences of all isolates indicated close relationship with the Californian chicken-origin isolate K1600657 and belonged to chicken-origin ARV cluster. Our data indicates that wild birds ARVs were derived from the chicken farms. This finding suggests that wild birds serve as natural carriers of such viruses for domestic poultry.
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spelling pubmed-88318412022-02-12 Isolation and Genomic Characterization of Avian Reovirus From Wild Birds in South Korea Kim, Sang-Won Choi, Yu-Ri Park, Jong-Yeol Wei, Bai Shang, Ke Zhang, Jun-Feng Jang, Hyung-Kwan Cha, Se-Yeoun Kang, Min Front Vet Sci Veterinary Science Avian reoviruses (ARVs) cause severe arthritis, tenosynovitis, pericarditis, and depressed growth in chickens, and these conditions have become increasingly frequent in recent years. Studies on the role of wild birds in the epidemiology of ARVs are insufficient. This study provides information about currently circulating ARVs in wild birds by gene detection using diagnostic RT-PCR, virus isolation, and genomic characterization. In this study, we isolated and identified 10 ARV isolates from 7,390 wild birds' fecal samples, including migratory bird species (bean goose, Eurasian teal, Indian spot-billed duck, and mallard duck) from 2015 to 2019 in South Korea. On comparing the amino acid sequences of the σC-encoding gene, most isolates, except A18-13, shared higher sequence similarity with the commercial vaccine isolate S1133 and Chinese isolates. However, the A18-13 isolate is similar to live attenuated vaccine av-S1133 and vaccine break isolates (SD09-1, LN09-1, and GX110116). For the p10- and p17-encoding genes, all isolates have identical fusion associated small transmembrane (FAST) protein and nuclear localization signal (SNL) motif to chicken-origin ARVs. Phylogenetic analysis of the amino acid sequences of the σC-encoding gene revealed that all isolates were belonged to genotypic cluster I. For the p10- and p17-encoding genes, the nucleotide sequences of all isolates indicated close relationship with commercial vaccine isolate S1133 and Chinese isolates. For the σNS-encoding gene, the nucleotide sequences of all isolates indicated close relationship with the Californian chicken-origin isolate K1600657 and belonged to chicken-origin ARV cluster. Our data indicates that wild birds ARVs were derived from the chicken farms. This finding suggests that wild birds serve as natural carriers of such viruses for domestic poultry. Frontiers Media S.A. 2022-01-28 /pmc/articles/PMC8831841/ /pubmed/35155656 http://dx.doi.org/10.3389/fvets.2022.794934 Text en Copyright © 2022 Kim, Choi, Park, Wei, Shang, Zhang, Jang, Cha and Kang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Kim, Sang-Won
Choi, Yu-Ri
Park, Jong-Yeol
Wei, Bai
Shang, Ke
Zhang, Jun-Feng
Jang, Hyung-Kwan
Cha, Se-Yeoun
Kang, Min
Isolation and Genomic Characterization of Avian Reovirus From Wild Birds in South Korea
title Isolation and Genomic Characterization of Avian Reovirus From Wild Birds in South Korea
title_full Isolation and Genomic Characterization of Avian Reovirus From Wild Birds in South Korea
title_fullStr Isolation and Genomic Characterization of Avian Reovirus From Wild Birds in South Korea
title_full_unstemmed Isolation and Genomic Characterization of Avian Reovirus From Wild Birds in South Korea
title_short Isolation and Genomic Characterization of Avian Reovirus From Wild Birds in South Korea
title_sort isolation and genomic characterization of avian reovirus from wild birds in south korea
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8831841/
https://www.ncbi.nlm.nih.gov/pubmed/35155656
http://dx.doi.org/10.3389/fvets.2022.794934
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