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One-Step Generation and Purification of Cell-Encapsulated Hydrogel Microsphere With an Easily Assembled Microfluidic Device

Cell-laden hydrogel microspheres with uniform size show great potential for tissue repair and drug screening applications. Droplet microfluidic systems have been widely used for the generation of cell-laden hydrogel microspheres. However, existing droplet microfluidic systems are mostly based on com...

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Autores principales: Zhang, Tao, Zhang, Hong, Zhou, Wuping, Jiang, Keming, Liu, Cong, Wang, Ru, Zhou, Yuanshuai, Zhang, Zhiqiang, Mei, Qian, Dong, Wen-Fei, Sun, Minxuan, Li, Haiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8831896/
https://www.ncbi.nlm.nih.gov/pubmed/35155414
http://dx.doi.org/10.3389/fbioe.2021.816089
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author Zhang, Tao
Zhang, Hong
Zhou, Wuping
Jiang, Keming
Liu, Cong
Wang, Ru
Zhou, Yuanshuai
Zhang, Zhiqiang
Mei, Qian
Dong, Wen-Fei
Sun, Minxuan
Li, Haiwen
author_facet Zhang, Tao
Zhang, Hong
Zhou, Wuping
Jiang, Keming
Liu, Cong
Wang, Ru
Zhou, Yuanshuai
Zhang, Zhiqiang
Mei, Qian
Dong, Wen-Fei
Sun, Minxuan
Li, Haiwen
author_sort Zhang, Tao
collection PubMed
description Cell-laden hydrogel microspheres with uniform size show great potential for tissue repair and drug screening applications. Droplet microfluidic systems have been widely used for the generation of cell-laden hydrogel microspheres. However, existing droplet microfluidic systems are mostly based on complex chips and are not compatible with well culture plates. Moreover, microspheres produced by droplet microfluidics need demulsification and purification from oil, which requires time and effort and may compromise cell viability. Herein, we present a simple one-step approach for producing and purifying hydrogel microspheres with an easily assembled microfluidic device. Droplets were generated and solidified in the device tubing. The obtained hydrogel microspheres were then transferred to a tissue culture plate filled with cell culture media and demulsified through evaporation of the oil at 37°C. The removal of oil caused the gelled microspheres to be released into the cell culture media. The encapsulated cells demonstrated good viability and grew into tumor spheroids in 12–14 days. Single cell-laden hydrogel microspheres were also obtained and grown into spheroid in 14 days. This one-step microsphere generation method shows good potential for applications in automated spheroid and organoid cultures as well as drug screening, and could potentially offer benefits for translation of cell/microgel technologies.
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spelling pubmed-88318962022-02-12 One-Step Generation and Purification of Cell-Encapsulated Hydrogel Microsphere With an Easily Assembled Microfluidic Device Zhang, Tao Zhang, Hong Zhou, Wuping Jiang, Keming Liu, Cong Wang, Ru Zhou, Yuanshuai Zhang, Zhiqiang Mei, Qian Dong, Wen-Fei Sun, Minxuan Li, Haiwen Front Bioeng Biotechnol Bioengineering and Biotechnology Cell-laden hydrogel microspheres with uniform size show great potential for tissue repair and drug screening applications. Droplet microfluidic systems have been widely used for the generation of cell-laden hydrogel microspheres. However, existing droplet microfluidic systems are mostly based on complex chips and are not compatible with well culture plates. Moreover, microspheres produced by droplet microfluidics need demulsification and purification from oil, which requires time and effort and may compromise cell viability. Herein, we present a simple one-step approach for producing and purifying hydrogel microspheres with an easily assembled microfluidic device. Droplets were generated and solidified in the device tubing. The obtained hydrogel microspheres were then transferred to a tissue culture plate filled with cell culture media and demulsified through evaporation of the oil at 37°C. The removal of oil caused the gelled microspheres to be released into the cell culture media. The encapsulated cells demonstrated good viability and grew into tumor spheroids in 12–14 days. Single cell-laden hydrogel microspheres were also obtained and grown into spheroid in 14 days. This one-step microsphere generation method shows good potential for applications in automated spheroid and organoid cultures as well as drug screening, and could potentially offer benefits for translation of cell/microgel technologies. Frontiers Media S.A. 2022-01-28 /pmc/articles/PMC8831896/ /pubmed/35155414 http://dx.doi.org/10.3389/fbioe.2021.816089 Text en Copyright © 2022 Zhang, Zhang, Zhou, Jiang, Liu, Wang, Zhou, Zhang, Mei, Dong, Sun and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Zhang, Tao
Zhang, Hong
Zhou, Wuping
Jiang, Keming
Liu, Cong
Wang, Ru
Zhou, Yuanshuai
Zhang, Zhiqiang
Mei, Qian
Dong, Wen-Fei
Sun, Minxuan
Li, Haiwen
One-Step Generation and Purification of Cell-Encapsulated Hydrogel Microsphere With an Easily Assembled Microfluidic Device
title One-Step Generation and Purification of Cell-Encapsulated Hydrogel Microsphere With an Easily Assembled Microfluidic Device
title_full One-Step Generation and Purification of Cell-Encapsulated Hydrogel Microsphere With an Easily Assembled Microfluidic Device
title_fullStr One-Step Generation and Purification of Cell-Encapsulated Hydrogel Microsphere With an Easily Assembled Microfluidic Device
title_full_unstemmed One-Step Generation and Purification of Cell-Encapsulated Hydrogel Microsphere With an Easily Assembled Microfluidic Device
title_short One-Step Generation and Purification of Cell-Encapsulated Hydrogel Microsphere With an Easily Assembled Microfluidic Device
title_sort one-step generation and purification of cell-encapsulated hydrogel microsphere with an easily assembled microfluidic device
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8831896/
https://www.ncbi.nlm.nih.gov/pubmed/35155414
http://dx.doi.org/10.3389/fbioe.2021.816089
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