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Nuclear Targeted Peptide Combined With Gambogic Acid for Synergistic Treatment of Breast Cancer
As a natural compound, gambogic acid (GA) emerged a shining multi-target antitumor activity in a variety of tumors. Whereas its poor solubility and non-specific effect to tumor blocked the clinical application of this drug. Herein, we reported a simple and effective strategy to construct liposome mo...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8832139/ https://www.ncbi.nlm.nih.gov/pubmed/35155383 http://dx.doi.org/10.3389/fchem.2021.821426 |
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author | Dang, Wenli Guo, Pan Song, Xunan Zhang, Ying Li, Nan Yu, Changxiang Xing, Bin Liu, Rui Jia, Xintao Zhang, Qingqing Feng, Xiaojiao Liu, Zhidong |
author_facet | Dang, Wenli Guo, Pan Song, Xunan Zhang, Ying Li, Nan Yu, Changxiang Xing, Bin Liu, Rui Jia, Xintao Zhang, Qingqing Feng, Xiaojiao Liu, Zhidong |
author_sort | Dang, Wenli |
collection | PubMed |
description | As a natural compound, gambogic acid (GA) emerged a shining multi-target antitumor activity in a variety of tumors. Whereas its poor solubility and non-specific effect to tumor blocked the clinical application of this drug. Herein, we reported a simple and effective strategy to construct liposome modified with nuclear targeted peptide CB5005N (VQRKRQKLMPC) via polyethylene glycol (PEG) linker to decrease the inherent limitations of GA and promote its anti-tumor activity. In this study, liposomes were prepared by thin film hydration method. The characterization of formulations contained particle size, Zeta potential, morphology and encapsulation efficiency. Further, in vitro cytotoxicity and uptake tests were investigated by 4T1 and MDA-MB-231 cells, and nuclear targeting capability was performed on MDA-MB-231 cells. In addition, the in vivo antitumor effect and biological distribution of formulations were tested in BALB/c female mice. The GA-loaded liposome modified by CB5005N showed small size, good uniformity, better targeting, higher anti-tumor efficiency, better tumor inhibition rate and lower toxicity to normal tissues than other groups. In vitro and in vivo research proved that CB5005N-GA-liposome exhibited excellent anti-tumor activity and significantly reduced toxicities. As a result, CB5005N-GA-liposome nano drug delivery system enhanced the tumor targeting and antitumor effects of GA, which provided a basis for its clinical application. |
format | Online Article Text |
id | pubmed-8832139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88321392022-02-12 Nuclear Targeted Peptide Combined With Gambogic Acid for Synergistic Treatment of Breast Cancer Dang, Wenli Guo, Pan Song, Xunan Zhang, Ying Li, Nan Yu, Changxiang Xing, Bin Liu, Rui Jia, Xintao Zhang, Qingqing Feng, Xiaojiao Liu, Zhidong Front Chem Chemistry As a natural compound, gambogic acid (GA) emerged a shining multi-target antitumor activity in a variety of tumors. Whereas its poor solubility and non-specific effect to tumor blocked the clinical application of this drug. Herein, we reported a simple and effective strategy to construct liposome modified with nuclear targeted peptide CB5005N (VQRKRQKLMPC) via polyethylene glycol (PEG) linker to decrease the inherent limitations of GA and promote its anti-tumor activity. In this study, liposomes were prepared by thin film hydration method. The characterization of formulations contained particle size, Zeta potential, morphology and encapsulation efficiency. Further, in vitro cytotoxicity and uptake tests were investigated by 4T1 and MDA-MB-231 cells, and nuclear targeting capability was performed on MDA-MB-231 cells. In addition, the in vivo antitumor effect and biological distribution of formulations were tested in BALB/c female mice. The GA-loaded liposome modified by CB5005N showed small size, good uniformity, better targeting, higher anti-tumor efficiency, better tumor inhibition rate and lower toxicity to normal tissues than other groups. In vitro and in vivo research proved that CB5005N-GA-liposome exhibited excellent anti-tumor activity and significantly reduced toxicities. As a result, CB5005N-GA-liposome nano drug delivery system enhanced the tumor targeting and antitumor effects of GA, which provided a basis for its clinical application. Frontiers Media S.A. 2022-01-28 /pmc/articles/PMC8832139/ /pubmed/35155383 http://dx.doi.org/10.3389/fchem.2021.821426 Text en Copyright © 2022 Dang, Guo, Song, Zhang, Li, Yu, Xing, Liu, Jia, Zhang, Feng and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Dang, Wenli Guo, Pan Song, Xunan Zhang, Ying Li, Nan Yu, Changxiang Xing, Bin Liu, Rui Jia, Xintao Zhang, Qingqing Feng, Xiaojiao Liu, Zhidong Nuclear Targeted Peptide Combined With Gambogic Acid for Synergistic Treatment of Breast Cancer |
title | Nuclear Targeted Peptide Combined With Gambogic Acid for Synergistic Treatment of Breast Cancer |
title_full | Nuclear Targeted Peptide Combined With Gambogic Acid for Synergistic Treatment of Breast Cancer |
title_fullStr | Nuclear Targeted Peptide Combined With Gambogic Acid for Synergistic Treatment of Breast Cancer |
title_full_unstemmed | Nuclear Targeted Peptide Combined With Gambogic Acid for Synergistic Treatment of Breast Cancer |
title_short | Nuclear Targeted Peptide Combined With Gambogic Acid for Synergistic Treatment of Breast Cancer |
title_sort | nuclear targeted peptide combined with gambogic acid for synergistic treatment of breast cancer |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8832139/ https://www.ncbi.nlm.nih.gov/pubmed/35155383 http://dx.doi.org/10.3389/fchem.2021.821426 |
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