Cargando…

Dioscin Reduces Vascular Damage in the Retina of db/db Mice by Inhibiting the VEGFA Signaling Pathway

Diabetic retinopathy (DR) is a complication of diabetes that has a serious impact on the quality of life of patients. VEGFA is necessary in the physiological state to maintain endothelial activity and physical properties of blood vessels. VEGFA plays an important role in the promotion of neovascular...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jun, Yang, Guang Yan, Sun, Hong Yan, Meng, Ting, Cheng, Chu Chu, Zhao, Hui Pan, Luo, Xiao Ling, Yang, Ming Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8832152/
https://www.ncbi.nlm.nih.gov/pubmed/35153764
http://dx.doi.org/10.3389/fphar.2021.811897
_version_ 1784648665463259136
author Wang, Jun
Yang, Guang Yan
Sun, Hong Yan
Meng, Ting
Cheng, Chu Chu
Zhao, Hui Pan
Luo, Xiao Ling
Yang, Ming Ming
author_facet Wang, Jun
Yang, Guang Yan
Sun, Hong Yan
Meng, Ting
Cheng, Chu Chu
Zhao, Hui Pan
Luo, Xiao Ling
Yang, Ming Ming
author_sort Wang, Jun
collection PubMed
description Diabetic retinopathy (DR) is a complication of diabetes that has a serious impact on the quality of life of patients. VEGFA is necessary in the physiological state to maintain endothelial activity and physical properties of blood vessels. VEGFA plays an important role in the promotion of neovascularization; therefore, inhibition of VEGFA can degrade the structure of blood vessels and reduce neovascularization. In the present study, HERB, a high-throughput experimental and reference-oriented database of herbal medicines, was used for compound mining targeting VEGFA. The compounds most likely to interact with VEGFA were screened by molecular docking. Next, the compounds were used to verify whether it could inhibit the activity of the VEGF signaling pathway in vitro and neovascularization in vivo. In vitro, we found that dioscin could inhibit the activation of the VEGFA–VEGFR2 signaling pathway and cell proliferation of human retinal microvascular endothelial cells in a high-glucose (HG) environment. A more important dioscin intervention inhibits the expression of pro-angiogenic factors in the retinas of db/db mice. In conclusion, our study indicates that dioscin reduces the vascular damage and the expression of pro-angiogenic factors in the retina of db/db mice and implies an important and potential application of dioscin for treatment of DR in clinics.
format Online
Article
Text
id pubmed-8832152
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88321522022-02-12 Dioscin Reduces Vascular Damage in the Retina of db/db Mice by Inhibiting the VEGFA Signaling Pathway Wang, Jun Yang, Guang Yan Sun, Hong Yan Meng, Ting Cheng, Chu Chu Zhao, Hui Pan Luo, Xiao Ling Yang, Ming Ming Front Pharmacol Pharmacology Diabetic retinopathy (DR) is a complication of diabetes that has a serious impact on the quality of life of patients. VEGFA is necessary in the physiological state to maintain endothelial activity and physical properties of blood vessels. VEGFA plays an important role in the promotion of neovascularization; therefore, inhibition of VEGFA can degrade the structure of blood vessels and reduce neovascularization. In the present study, HERB, a high-throughput experimental and reference-oriented database of herbal medicines, was used for compound mining targeting VEGFA. The compounds most likely to interact with VEGFA were screened by molecular docking. Next, the compounds were used to verify whether it could inhibit the activity of the VEGF signaling pathway in vitro and neovascularization in vivo. In vitro, we found that dioscin could inhibit the activation of the VEGFA–VEGFR2 signaling pathway and cell proliferation of human retinal microvascular endothelial cells in a high-glucose (HG) environment. A more important dioscin intervention inhibits the expression of pro-angiogenic factors in the retinas of db/db mice. In conclusion, our study indicates that dioscin reduces the vascular damage and the expression of pro-angiogenic factors in the retina of db/db mice and implies an important and potential application of dioscin for treatment of DR in clinics. Frontiers Media S.A. 2022-01-28 /pmc/articles/PMC8832152/ /pubmed/35153764 http://dx.doi.org/10.3389/fphar.2021.811897 Text en Copyright © 2022 Wang, Yang, Sun, Meng, Cheng, Zhao, Luo and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Wang, Jun
Yang, Guang Yan
Sun, Hong Yan
Meng, Ting
Cheng, Chu Chu
Zhao, Hui Pan
Luo, Xiao Ling
Yang, Ming Ming
Dioscin Reduces Vascular Damage in the Retina of db/db Mice by Inhibiting the VEGFA Signaling Pathway
title Dioscin Reduces Vascular Damage in the Retina of db/db Mice by Inhibiting the VEGFA Signaling Pathway
title_full Dioscin Reduces Vascular Damage in the Retina of db/db Mice by Inhibiting the VEGFA Signaling Pathway
title_fullStr Dioscin Reduces Vascular Damage in the Retina of db/db Mice by Inhibiting the VEGFA Signaling Pathway
title_full_unstemmed Dioscin Reduces Vascular Damage in the Retina of db/db Mice by Inhibiting the VEGFA Signaling Pathway
title_short Dioscin Reduces Vascular Damage in the Retina of db/db Mice by Inhibiting the VEGFA Signaling Pathway
title_sort dioscin reduces vascular damage in the retina of db/db mice by inhibiting the vegfa signaling pathway
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8832152/
https://www.ncbi.nlm.nih.gov/pubmed/35153764
http://dx.doi.org/10.3389/fphar.2021.811897
work_keys_str_mv AT wangjun dioscinreducesvasculardamageintheretinaofdbdbmicebyinhibitingthevegfasignalingpathway
AT yangguangyan dioscinreducesvasculardamageintheretinaofdbdbmicebyinhibitingthevegfasignalingpathway
AT sunhongyan dioscinreducesvasculardamageintheretinaofdbdbmicebyinhibitingthevegfasignalingpathway
AT mengting dioscinreducesvasculardamageintheretinaofdbdbmicebyinhibitingthevegfasignalingpathway
AT chengchuchu dioscinreducesvasculardamageintheretinaofdbdbmicebyinhibitingthevegfasignalingpathway
AT zhaohuipan dioscinreducesvasculardamageintheretinaofdbdbmicebyinhibitingthevegfasignalingpathway
AT luoxiaoling dioscinreducesvasculardamageintheretinaofdbdbmicebyinhibitingthevegfasignalingpathway
AT yangmingming dioscinreducesvasculardamageintheretinaofdbdbmicebyinhibitingthevegfasignalingpathway