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The Natural Cytotoxicity Receptor NKp44 (NCR2, CD336) Is Expressed on the Majority of Porcine NK Cells Ex Vivo Without Stimulation

Natural killer (NK) cells have been studied extensively in humans and mice for their vital role in the vertebrate innate immune system. They are known to rapidly eliminate tumors or virus infected cells in an immune response utilizing their lytic properties. The natural cytotoxicity receptors (NCRs)...

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Autores principales: Mair, Kerstin H., Crossman, Assiatu J., Wagner, Bettina, Babasyan, Susanna, Noronha, Leela, Boyd, Patricia, Zarlenga, Dante, Stadler, Maria, van Dongen, Katinka A., Gerner, Wilhelm, Saalmüller, Armin, Lunney, Joan K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8832162/
https://www.ncbi.nlm.nih.gov/pubmed/35154097
http://dx.doi.org/10.3389/fimmu.2022.767530
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author Mair, Kerstin H.
Crossman, Assiatu J.
Wagner, Bettina
Babasyan, Susanna
Noronha, Leela
Boyd, Patricia
Zarlenga, Dante
Stadler, Maria
van Dongen, Katinka A.
Gerner, Wilhelm
Saalmüller, Armin
Lunney, Joan K.
author_facet Mair, Kerstin H.
Crossman, Assiatu J.
Wagner, Bettina
Babasyan, Susanna
Noronha, Leela
Boyd, Patricia
Zarlenga, Dante
Stadler, Maria
van Dongen, Katinka A.
Gerner, Wilhelm
Saalmüller, Armin
Lunney, Joan K.
author_sort Mair, Kerstin H.
collection PubMed
description Natural killer (NK) cells have been studied extensively in humans and mice for their vital role in the vertebrate innate immune system. They are known to rapidly eliminate tumors or virus infected cells in an immune response utilizing their lytic properties. The natural cytotoxicity receptors (NCRs) NKp30 (NCR3), NKp44 (NCR2), and NKp46 (NCR1) are important mediators of NK-cell cytotoxicity. NKp44 expression was reported for NK cells in humans as well as in some non-human primates and found exclusively on activated NK cells. Previously, no information was available on NKp44 protein expression and its role in porcine lymphocytes due to the lack of species-specific monoclonal antibodies (mAbs). For this study, porcine-specific anti-NKp44 mAbs were generated and their reactivity was tested on blood and tissue derived NK cells in pigs of different age classes. Interestingly, NKp44 expression was detected ex vivo already on resting NK cells; moreover, the frequency of NKp44(+) NK cells was higher than that of NKp46(+) NK cells in most animals analyzed. Upon in vitro stimulation with IL-2 or IL-15, the frequency of NKp44(+) NK cells, as well as the intensity of NKp44 expression at the single cell level, were increased. Since little is known about swine NK cells, the generation of a mAb (clone 54-1) against NKp44 will greatly aid in elucidating the mechanisms underlying the differentiation, functionality, and activation of porcine NK cells.
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spelling pubmed-88321622022-02-12 The Natural Cytotoxicity Receptor NKp44 (NCR2, CD336) Is Expressed on the Majority of Porcine NK Cells Ex Vivo Without Stimulation Mair, Kerstin H. Crossman, Assiatu J. Wagner, Bettina Babasyan, Susanna Noronha, Leela Boyd, Patricia Zarlenga, Dante Stadler, Maria van Dongen, Katinka A. Gerner, Wilhelm Saalmüller, Armin Lunney, Joan K. Front Immunol Immunology Natural killer (NK) cells have been studied extensively in humans and mice for their vital role in the vertebrate innate immune system. They are known to rapidly eliminate tumors or virus infected cells in an immune response utilizing their lytic properties. The natural cytotoxicity receptors (NCRs) NKp30 (NCR3), NKp44 (NCR2), and NKp46 (NCR1) are important mediators of NK-cell cytotoxicity. NKp44 expression was reported for NK cells in humans as well as in some non-human primates and found exclusively on activated NK cells. Previously, no information was available on NKp44 protein expression and its role in porcine lymphocytes due to the lack of species-specific monoclonal antibodies (mAbs). For this study, porcine-specific anti-NKp44 mAbs were generated and their reactivity was tested on blood and tissue derived NK cells in pigs of different age classes. Interestingly, NKp44 expression was detected ex vivo already on resting NK cells; moreover, the frequency of NKp44(+) NK cells was higher than that of NKp46(+) NK cells in most animals analyzed. Upon in vitro stimulation with IL-2 or IL-15, the frequency of NKp44(+) NK cells, as well as the intensity of NKp44 expression at the single cell level, were increased. Since little is known about swine NK cells, the generation of a mAb (clone 54-1) against NKp44 will greatly aid in elucidating the mechanisms underlying the differentiation, functionality, and activation of porcine NK cells. Frontiers Media S.A. 2022-01-28 /pmc/articles/PMC8832162/ /pubmed/35154097 http://dx.doi.org/10.3389/fimmu.2022.767530 Text en Copyright © 2022 Mair, Crossman, Wagner, Babasyan, Noronha, Boyd, Zarlenga, Stadler, van Dongen, Gerner, Saalmüller and Lunney https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Mair, Kerstin H.
Crossman, Assiatu J.
Wagner, Bettina
Babasyan, Susanna
Noronha, Leela
Boyd, Patricia
Zarlenga, Dante
Stadler, Maria
van Dongen, Katinka A.
Gerner, Wilhelm
Saalmüller, Armin
Lunney, Joan K.
The Natural Cytotoxicity Receptor NKp44 (NCR2, CD336) Is Expressed on the Majority of Porcine NK Cells Ex Vivo Without Stimulation
title The Natural Cytotoxicity Receptor NKp44 (NCR2, CD336) Is Expressed on the Majority of Porcine NK Cells Ex Vivo Without Stimulation
title_full The Natural Cytotoxicity Receptor NKp44 (NCR2, CD336) Is Expressed on the Majority of Porcine NK Cells Ex Vivo Without Stimulation
title_fullStr The Natural Cytotoxicity Receptor NKp44 (NCR2, CD336) Is Expressed on the Majority of Porcine NK Cells Ex Vivo Without Stimulation
title_full_unstemmed The Natural Cytotoxicity Receptor NKp44 (NCR2, CD336) Is Expressed on the Majority of Porcine NK Cells Ex Vivo Without Stimulation
title_short The Natural Cytotoxicity Receptor NKp44 (NCR2, CD336) Is Expressed on the Majority of Porcine NK Cells Ex Vivo Without Stimulation
title_sort natural cytotoxicity receptor nkp44 (ncr2, cd336) is expressed on the majority of porcine nk cells ex vivo without stimulation
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8832162/
https://www.ncbi.nlm.nih.gov/pubmed/35154097
http://dx.doi.org/10.3389/fimmu.2022.767530
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