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Actomyosin-Assisted Pulling of Lipid Nanotubes from Lipid Vesicles and Cells

[Image: see text] Molecular motors are pivotal for intracellular transport as well as cell motility and have great potential to be put to use outside cells. Here, we exploit engineered motor proteins in combination with self-assembly of actin filaments to actively pull lipid nanotubes from giant uni...

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Detalles Bibliográficos
Autores principales: Jahnke, Kevin, Maurer, Stefan J., Weber, Cornelia, Bücher, Jochen Estebano Hernandez, Schoenit, Andreas, D’Este, Elisa, Cavalcanti-Adam, Elisabetta Ada, Göpfrich, Kerstin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8832490/
https://www.ncbi.nlm.nih.gov/pubmed/35089720
http://dx.doi.org/10.1021/acs.nanolett.1c04254
Descripción
Sumario:[Image: see text] Molecular motors are pivotal for intracellular transport as well as cell motility and have great potential to be put to use outside cells. Here, we exploit engineered motor proteins in combination with self-assembly of actin filaments to actively pull lipid nanotubes from giant unilamellar vesicles (GUVs). In particular, actin filaments are bound to the outer GUV membrane and the GUVs are seeded on a heavy meromyosin-coated substrate. Upon addition of ATP, hollow lipid nanotubes with a length of tens of micrometer are pulled from single GUVs due to the motor activity. We employ the same mechanism to pull lipid nanotubes from different types of cells. We find that the length and number of nanotubes critically depends on the cell type, whereby suspension cells form bigger networks than adherent cells. This suggests that molecular machines can be used to exert forces on living cells to probe membrane-to-cortex attachment.