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Integrative bioinformatics and experimental analysis revealed TEAD as novel prognostic target for hepatocellular carcinoma and its roles in ferroptosis regulation

Objective: Transcriptional enhanced associate domain (TEAD) family consists of four members TEAD1/2/3/4 that regulate cell growth, stem cell functions and organ development. As the downstream of Hippo signaling pathway, TEAD family is involved in the progression of several cancers. However, the prec...

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Autores principales: Ren, Xinxin, Wang, Xiang, Yan, Yuanliang, Chen, Xi, Cai, Yuan, Liang, Qiuju, Peng, Bi, Xu, Zhijie, He, Qingchun, Kang, Fanhua, Li, Jianbo, Zhang, Wenqin, Hong, Qianhui, Peng, Jinwu, Xiao, Muzhang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8833120/
https://www.ncbi.nlm.nih.gov/pubmed/35077390
http://dx.doi.org/10.18632/aging.203853
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author Ren, Xinxin
Wang, Xiang
Yan, Yuanliang
Chen, Xi
Cai, Yuan
Liang, Qiuju
Peng, Bi
Xu, Zhijie
He, Qingchun
Kang, Fanhua
Li, Jianbo
Zhang, Wenqin
Hong, Qianhui
Peng, Jinwu
Xiao, Muzhang
author_facet Ren, Xinxin
Wang, Xiang
Yan, Yuanliang
Chen, Xi
Cai, Yuan
Liang, Qiuju
Peng, Bi
Xu, Zhijie
He, Qingchun
Kang, Fanhua
Li, Jianbo
Zhang, Wenqin
Hong, Qianhui
Peng, Jinwu
Xiao, Muzhang
author_sort Ren, Xinxin
collection PubMed
description Objective: Transcriptional enhanced associate domain (TEAD) family consists of four members TEAD1/2/3/4 that regulate cell growth, stem cell functions and organ development. As the downstream of Hippo signaling pathway, TEAD family is involved in the progression of several cancers. However, the precise biology functions of TEAD family in hepatocellular carcinoma (HCC) have not been reported yet. Methods: We apply bioinformatics analysis based on databases including UALCAN, Oncomine, GEPIA, Kaplan-Meier plotter, WebGestalt, cBioPortal, TIMER2.0, and in vitro experimental evidence to identify the exact roles of TEAD family in HCC. Results: The results indicated that TEAD2/4 were significantly upregulated in HCC compared with normal tissues. Downregulated of TEAD2 could promote the death of HCC cells through inducing ferroptosis by iron accumulation and subsequent oxidative damage. According to the Kaplan-Meier plotter database, we found that the high expression of TEAD2 was significantly associated with poor disease-specific survival, overall survival, progression-free survival and relapse-free survival. In aspect of cancer immunity, Tumor Immune Estimation Resource algorithm showed that the expression of TEAD family members was obviously related to multiple of infiltrating immune cells including macrophages, neutrophils, dendritic cells, B cells, CD8+ T cells and CD4+ T cells. Finally, we conducted the functional enrichment analysis including protein-protein interaction network, gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway based on the TEAD family-associated coexpression genes. Conclusion: The study provided deep insight information of TEAD family in the diagnostic and prognostic evaluation of HCC patients.
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spelling pubmed-88331202022-02-14 Integrative bioinformatics and experimental analysis revealed TEAD as novel prognostic target for hepatocellular carcinoma and its roles in ferroptosis regulation Ren, Xinxin Wang, Xiang Yan, Yuanliang Chen, Xi Cai, Yuan Liang, Qiuju Peng, Bi Xu, Zhijie He, Qingchun Kang, Fanhua Li, Jianbo Zhang, Wenqin Hong, Qianhui Peng, Jinwu Xiao, Muzhang Aging (Albany NY) Research Paper Objective: Transcriptional enhanced associate domain (TEAD) family consists of four members TEAD1/2/3/4 that regulate cell growth, stem cell functions and organ development. As the downstream of Hippo signaling pathway, TEAD family is involved in the progression of several cancers. However, the precise biology functions of TEAD family in hepatocellular carcinoma (HCC) have not been reported yet. Methods: We apply bioinformatics analysis based on databases including UALCAN, Oncomine, GEPIA, Kaplan-Meier plotter, WebGestalt, cBioPortal, TIMER2.0, and in vitro experimental evidence to identify the exact roles of TEAD family in HCC. Results: The results indicated that TEAD2/4 were significantly upregulated in HCC compared with normal tissues. Downregulated of TEAD2 could promote the death of HCC cells through inducing ferroptosis by iron accumulation and subsequent oxidative damage. According to the Kaplan-Meier plotter database, we found that the high expression of TEAD2 was significantly associated with poor disease-specific survival, overall survival, progression-free survival and relapse-free survival. In aspect of cancer immunity, Tumor Immune Estimation Resource algorithm showed that the expression of TEAD family members was obviously related to multiple of infiltrating immune cells including macrophages, neutrophils, dendritic cells, B cells, CD8+ T cells and CD4+ T cells. Finally, we conducted the functional enrichment analysis including protein-protein interaction network, gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway based on the TEAD family-associated coexpression genes. Conclusion: The study provided deep insight information of TEAD family in the diagnostic and prognostic evaluation of HCC patients. Impact Journals 2022-01-25 /pmc/articles/PMC8833120/ /pubmed/35077390 http://dx.doi.org/10.18632/aging.203853 Text en Copyright: © 2022 Ren et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Ren, Xinxin
Wang, Xiang
Yan, Yuanliang
Chen, Xi
Cai, Yuan
Liang, Qiuju
Peng, Bi
Xu, Zhijie
He, Qingchun
Kang, Fanhua
Li, Jianbo
Zhang, Wenqin
Hong, Qianhui
Peng, Jinwu
Xiao, Muzhang
Integrative bioinformatics and experimental analysis revealed TEAD as novel prognostic target for hepatocellular carcinoma and its roles in ferroptosis regulation
title Integrative bioinformatics and experimental analysis revealed TEAD as novel prognostic target for hepatocellular carcinoma and its roles in ferroptosis regulation
title_full Integrative bioinformatics and experimental analysis revealed TEAD as novel prognostic target for hepatocellular carcinoma and its roles in ferroptosis regulation
title_fullStr Integrative bioinformatics and experimental analysis revealed TEAD as novel prognostic target for hepatocellular carcinoma and its roles in ferroptosis regulation
title_full_unstemmed Integrative bioinformatics and experimental analysis revealed TEAD as novel prognostic target for hepatocellular carcinoma and its roles in ferroptosis regulation
title_short Integrative bioinformatics and experimental analysis revealed TEAD as novel prognostic target for hepatocellular carcinoma and its roles in ferroptosis regulation
title_sort integrative bioinformatics and experimental analysis revealed tead as novel prognostic target for hepatocellular carcinoma and its roles in ferroptosis regulation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8833120/
https://www.ncbi.nlm.nih.gov/pubmed/35077390
http://dx.doi.org/10.18632/aging.203853
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