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Time-resolved DEER EPR and solid-state NMR afford kinetic and structural elucidation of substrate binding to Ca(2+)-ligated calmodulin

Recent advances in rapid mixing and freeze quenching have opened the path for time-resolved electron paramagnetic resonance (EPR)-based double electron-electron resonance (DEER) and solid-state NMR of protein–substrate interactions. DEER, in conjunction with phase memory time filtering to quantitati...

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Detalles Bibliográficos
Autores principales:	Schmidt, Thomas, Jeon, Jaekyun, Yau, Wai-Ming, Schwieters, Charles D., Tycko, Robert, Clore, G. Marius
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	National Academy of Sciences 2022
Materias:	Biological Sciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8833187/ https://www.ncbi.nlm.nih.gov/pubmed/35105816 http://dx.doi.org/10.1073/pnas.2122308119

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