Resveratrol Improves Intestinal Morphology and Anti-Oxidation Ability in Deoxynivalenol-Challenged Piglets

SIMPLE SUMMARY: Deoxynivalenol (DON)-contaminated feed may cause anorexia, vomiting, immunosuppression, and intestinal dysfunction in pigs, which would lead to growth retardation and great losses in the pig industry. In this study, the effects of resveratrol (RES) on growth performance, the intestinal barrier, antioxidant capacity, and mitochondrial function in weaned pigs fed with DON-contaminated diets were investigated. Dietary supplementation with resveratrol increased the average daily feed intake of piglets. Diets supplemented with resveratrol increased the villus height and the ratio of the jejunum villus height to crypt depth, increased the activities of superoxide dismutase (SOD), and increased the total antioxidant capacity in the jejunum mucosa. After being supplemented with RES, the level of reactive oxygen species (ROS) in mitochondria was decreased, while the mitochondrial membrane potential in the jejunum was increased. In conclusion, these results suggested that resveratrol effectively relieved DON-induced oxidative stress in weaned piglets, improved intestinal barrier function, enhanced mitochondrial function, and improved the growth performance of piglets. ABSTRACT: This study aimed to investigate the potential effects of resveratrol (RES) on intestinal function and oxidative stress in deoxynivalenol (DON)-challenged piglets. Twenty-four healthy Duroc × Yorkshire × Landrace weaned piglets at the age of 28 ± 1 days were randomly divided into four groups with six repetitions per group. The four groups were as follows: the control group (CON), fed with a basic diet; the RES group, fed with a basal diet + 300 mg/kg RES; the DON group, fed with a basal diet containing 2.65 mg/kg DON; and the DON + RES group, fed with a basal diet containing 2.65 mg/kg DON + 300 mg/kg RES. The results showed that the growth performance and intestinal function of DON-challenged piglets were significantly decreased (p < 0.05). Compared with the DON group, the average daily feed intake of piglets in the DON + RES group was significantly increased (p < 0.05). Additionally, dietary RES ameliorated DON-induced intestinal morphology impairment, as indicated by the increased (p < 0.05) jejunal villi height and the ratio of the jejunal villi height/crypt depth. Furthermore, after the addition of RES, the activities of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in the jejunum mucosa were significantly increased, and the content of malondialdehyde (MDA) was significantly declined (p < 0.05). In addition, the level of reactive oxygen species (ROS) in the mitochondria was significantly reduced by RES, while the mitochondrial membrane potential in jejunum was significantly increased by RES (p < 0.05). However, there was no obvious difference between DON + RES and DON groups on average daily gain and the ratio of feed togain, except for the significant inhibition of average daily feed intake (p < 0.05). In conclusion, RES could effectively alleviate the DON-induced oxidative stress on weaned piglets, and reduce the damage to mitochondria and intestinal morphology, so as to improve the growth performance of piglets.