Time Series Ovarian Transcriptome Analyses of the Porcine Estrous Cycle Reveals Gene Expression Changes during Steroid Metabolism and Corpus Luteum Development

SIMPLE SUMMARY: The estrous cycle, which is divided into follicular and luteal phases based on ovulation, is influenced by reproductive hormones which affect reproduction and cause changes in the reproductive system of the pig. As the main reproductive organ, the ovary is involved in ovulation and changes in the corpus luteum. We aimed to identify dynamic changes in gene expression through differentially expressed gene profiling and to provide a comprehensive understanding of the molecular mechanisms that occur in the pig ovary during the estrous cycle. The transcriptome analysis revealed that the dynamic change in gene expression was more activated in the luteal phase than in the follicular phase. Functional analysis revealed that the metestrus and diestrus periods are important in preparation for pregnancy or the next estrous cycle after ovulation. ABSTRACT: The porcine estrous cycle is influenced by reproductive hormones, which affect porcine reproduction and result in physiological changes in the reproductive organs. The ovary is involved in ovulation, luteinization, corpus luteum development, and luteolysis. Here, we aimed to provide a comprehensive understanding of the gene expression patterns in porcine ovarian transcriptomes during the estrous cycle through differentially expressed genes profiling and description of molecular mechanisms. The transcriptomes of porcine ovary were obtained during the estrous cycle at three-day intervals from day 0 to day 18 using RNA-seq. At seven time points of the estrous cycle, 4414 DEG were identified; these were classified into three clusters according to their expression patterns. During the late metestrus and diestrus periods, the expression in cluster 1 increased rapidly, and steroid biosynthesis was significant in the pathway. Cluster 2 gene expression patterns represented the cytokine–cytokine receptor interaction in significant pathways. In cluster 3, the hedgehog signaling pathway was selected as the significant pathway. Our study exhibited dynamic gene expression changes with these three different patterns of cluster 1, 2, and 3. The results helped identify the functions and related significant genes especially during the late metestrus and diestrus periods in the estrous cycle.