Use of Blood Lactate in Assessment of Manual Capture Techniques of Zoo-Housed Crocodilians

SIMPLE SUMMARY: This study aimed to clarify the relationship between manual capture techniques, blood lactate levels, and other varying factors in order to minimize physiological stress during manual capture and restraint events of zoo-housed crocodilians. While following the St. Augustine Alligator Farm Zoological Park’s capture and restraint protocol, 53 blood samples from ten crocodilian species were collected and analyzed for lactate. These measurements were then related to specific behavioral and extrinsic factors. We sought to define blood lactate as a new welfare marker for crocodilians in zoos. Based on our findings, we are able to recommend some best practices for manual capture methods for crocodilians. ABSTRACT: Species-specific welfare indicators are important in promoting positive welfare for zoo animals. Reptiles are a notoriously understudied group in regards to behavior, welfare needs, and husbandry requirements. Using opportunistically obtained samples, we evaluated how blood lactate is affected by variation in manual capture and restraint in crocodilians. Lactate is an indicator of anerobic metabolism in reptiles. It offers a relatively simple and indirect way to assess physiological stress. Determining the best way to reduce struggling during capture and associated stress is of great importance to these species’ overall welfare. Blood samples (N = 53) were collected from 10 different species of crocodilians. It was found that age class was a significant predictor variable of lactate levels after capture, and longer handling time seemed to cause an increase in lactate. Finally, draining enclosure pools for a small number of the captures was associated with higher lactate levels compared to other capture factors that were recorded. This study showed that welfare of captive crocodilians could be improved by shortening the duration of physical restraint events when possible. Developing objective measures of welfare and establishing baseline recommendations for care and handling of crocodilians will ultimately promote and improve their wellbeing, along with that of other understudied reptiles in zoos.