Effects of Leptin, Growth Hormone and Photoperiod on Pituitary SOCS-3 Expression in Sheep

SIMPLE SUMMARY: Maintaining energy homeostasis requires numerous processes and interactions between many systems. This study investigated the relationships between leptin, growth hormone (GH), and factors involved in cell signaling, such as suppressors of cytokine signaling-3 (SOCS-3). Exogenous or endogenous factors affecting these relationships may have different origins, and their interactions are determined in this study. The finding that the length of day significantly influenced the concentrations of growth hormone as well as pituitary leptin- and GH-dependent SOCS-3 expressions suggests that photoperiod plays an important role in regulating the physiological processes underlying adaptive phenomena in response to changing seasons in sheep, which facilitates energy homeostasis despite changing external and internal conditions. ABSTRACT: This study examined how leptin affects growth hormone (GH) release and investigated the effects of leptin, GH, and day length on the suppressor of cytokine signaling-3 (SOCS-3) mRNA levels in the adenohypophyses of sheep. The study consisted of two experiments. The first experiment was conducted during long (LD) and short (SD) days. Within-season and replicate sheep were centrally infused with Ringer-Locke buffer or leptin three times at 60-min intervals at the beginning of experiments. The second experiment involved adenohypophyses collected from sheep that were euthanized in May or November. Pituitary explants were treated with medium alone (Control) or medium with leptin or GH at different concentrations and incubated for various times. The results of the first experiment indicated GH concentrations were seasonally dependent and that leptin had no effect on GH secretion. The results of the second experiment indicated a stronger influence of leptin on the expression of SOCS-3 during the SD season than the LD season. During SDs, significant effects of both GH doses on SOCS-3 expression were observed. These results indicate a strong association between leptin, GH, and SOCS-3, which may explain the disruption of SOCS-3 leptin and GH signaling and the dominant effect of photoperiod on the above relationships.