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Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome

The etiology of dry mouth conditions is multi-faceted. Patients radiated after head and neck cancer (HNC) and those with primary Sjögren’s syndrome (pSS) share many of the same symptoms despite different causes. With the aim of better understanding the pathophysiology and biochemical processes behin...

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Autores principales: Hynne, Håvard, Sandås, Elise Mørk, Elgstøen, Katja Benedikte Prestø, Rootwelt, Helge, Utheim, Tor P., Galtung, Hilde Kanli, Jensen, Janicke Liaaen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8833893/
https://www.ncbi.nlm.nih.gov/pubmed/35159133
http://dx.doi.org/10.3390/cells11030323
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author Hynne, Håvard
Sandås, Elise Mørk
Elgstøen, Katja Benedikte Prestø
Rootwelt, Helge
Utheim, Tor P.
Galtung, Hilde Kanli
Jensen, Janicke Liaaen
author_facet Hynne, Håvard
Sandås, Elise Mørk
Elgstøen, Katja Benedikte Prestø
Rootwelt, Helge
Utheim, Tor P.
Galtung, Hilde Kanli
Jensen, Janicke Liaaen
author_sort Hynne, Håvard
collection PubMed
description The etiology of dry mouth conditions is multi-faceted. Patients radiated after head and neck cancer (HNC) and those with primary Sjögren’s syndrome (pSS) share many of the same symptoms despite different causes. With the aim of better understanding the pathophysiology and biochemical processes behind dry mouth with different etiologies, we investigated the metabolic profile of 10 HNC patients, 9 pSS patients and 10 healthy controls using high-performance liquid chromatography-high resolution mass spectrometry (HPLC-MS) metabolomics. Principal component analysis (PCA) revealed different metabolic profiles when comparing all subjects included in the study. Both patient groups showed higher ratios of several pyrimidine nucleotides and nucleosides when compared to controls. This finding may indicate that purinergic signaling plays a role in dry mouth conditions. Moreover, significantly increased levels of DL-3-aminoisobutyric acid were found in HNC patients when compared to controls, and a similar tendency was observed in the pSS patients. Furthermore, a dysregulation in amino acid metabolism was observed in both patient groups. In conclusion, metabolomics analysis showed separate metabolic profiles for HNC and pSS patients as compared to controls that could be useful in diagnostics and for elucidating the different pathophysiologies. The demonstrated dysregulation of pyrimidine nucleotides and levels of metabolites derived from amino acids in the patient groups should be studied further.
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spelling pubmed-88338932022-02-12 Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome Hynne, Håvard Sandås, Elise Mørk Elgstøen, Katja Benedikte Prestø Rootwelt, Helge Utheim, Tor P. Galtung, Hilde Kanli Jensen, Janicke Liaaen Cells Article The etiology of dry mouth conditions is multi-faceted. Patients radiated after head and neck cancer (HNC) and those with primary Sjögren’s syndrome (pSS) share many of the same symptoms despite different causes. With the aim of better understanding the pathophysiology and biochemical processes behind dry mouth with different etiologies, we investigated the metabolic profile of 10 HNC patients, 9 pSS patients and 10 healthy controls using high-performance liquid chromatography-high resolution mass spectrometry (HPLC-MS) metabolomics. Principal component analysis (PCA) revealed different metabolic profiles when comparing all subjects included in the study. Both patient groups showed higher ratios of several pyrimidine nucleotides and nucleosides when compared to controls. This finding may indicate that purinergic signaling plays a role in dry mouth conditions. Moreover, significantly increased levels of DL-3-aminoisobutyric acid were found in HNC patients when compared to controls, and a similar tendency was observed in the pSS patients. Furthermore, a dysregulation in amino acid metabolism was observed in both patient groups. In conclusion, metabolomics analysis showed separate metabolic profiles for HNC and pSS patients as compared to controls that could be useful in diagnostics and for elucidating the different pathophysiologies. The demonstrated dysregulation of pyrimidine nucleotides and levels of metabolites derived from amino acids in the patient groups should be studied further. MDPI 2022-01-19 /pmc/articles/PMC8833893/ /pubmed/35159133 http://dx.doi.org/10.3390/cells11030323 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hynne, Håvard
Sandås, Elise Mørk
Elgstøen, Katja Benedikte Prestø
Rootwelt, Helge
Utheim, Tor P.
Galtung, Hilde Kanli
Jensen, Janicke Liaaen
Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome
title Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome
title_full Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome
title_fullStr Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome
title_full_unstemmed Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome
title_short Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome
title_sort saliva metabolomics in dry mouth patients with head and neck cancer or sjögren’s syndrome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8833893/
https://www.ncbi.nlm.nih.gov/pubmed/35159133
http://dx.doi.org/10.3390/cells11030323
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