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Cryptococcal Protease(s) and the Activation of SARS-CoV-2 Spike (S) Protein
In this contribution, we report on the possibility that cryptococcal protease(s) could activate the SARS-CoV-2 spike (S) protein. The S protein is documented to have a unique four-amino-acid sequence (underlined, SPRRAR↓S) at the interface between the S1 and S2 sites, that serves as a cleavage site...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834071/ https://www.ncbi.nlm.nih.gov/pubmed/35159253 http://dx.doi.org/10.3390/cells11030437 |
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author | Mjokane, Nozethu Maliehe, Maphori Folorunso, Olufemi S. Ogundeji, Adepemi O. Gcilitshana, Onele M. N. Albertyn, Jacobus Pohl, Carolina H. Sebolai, Olihile M. |
author_facet | Mjokane, Nozethu Maliehe, Maphori Folorunso, Olufemi S. Ogundeji, Adepemi O. Gcilitshana, Onele M. N. Albertyn, Jacobus Pohl, Carolina H. Sebolai, Olihile M. |
author_sort | Mjokane, Nozethu |
collection | PubMed |
description | In this contribution, we report on the possibility that cryptococcal protease(s) could activate the SARS-CoV-2 spike (S) protein. The S protein is documented to have a unique four-amino-acid sequence (underlined, SPRRAR↓S) at the interface between the S1 and S2 sites, that serves as a cleavage site for the human protease, furin. We compared the biochemical efficiency of cryptococcal protease(s) and furin to mediate the proteolytic cleavage of the S1/S2 site in a fluorogenic peptide. We show that cryptococcal protease(s) processes this site in a manner comparable to the efficiency of furin (p > 0.581). We conclude the paper by discussing the impact of these findings in the context of a SARS-CoV-2 disease manifesting while there is an underlying cryptococcal infection. |
format | Online Article Text |
id | pubmed-8834071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88340712022-02-12 Cryptococcal Protease(s) and the Activation of SARS-CoV-2 Spike (S) Protein Mjokane, Nozethu Maliehe, Maphori Folorunso, Olufemi S. Ogundeji, Adepemi O. Gcilitshana, Onele M. N. Albertyn, Jacobus Pohl, Carolina H. Sebolai, Olihile M. Cells Article In this contribution, we report on the possibility that cryptococcal protease(s) could activate the SARS-CoV-2 spike (S) protein. The S protein is documented to have a unique four-amino-acid sequence (underlined, SPRRAR↓S) at the interface between the S1 and S2 sites, that serves as a cleavage site for the human protease, furin. We compared the biochemical efficiency of cryptococcal protease(s) and furin to mediate the proteolytic cleavage of the S1/S2 site in a fluorogenic peptide. We show that cryptococcal protease(s) processes this site in a manner comparable to the efficiency of furin (p > 0.581). We conclude the paper by discussing the impact of these findings in the context of a SARS-CoV-2 disease manifesting while there is an underlying cryptococcal infection. MDPI 2022-01-27 /pmc/articles/PMC8834071/ /pubmed/35159253 http://dx.doi.org/10.3390/cells11030437 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mjokane, Nozethu Maliehe, Maphori Folorunso, Olufemi S. Ogundeji, Adepemi O. Gcilitshana, Onele M. N. Albertyn, Jacobus Pohl, Carolina H. Sebolai, Olihile M. Cryptococcal Protease(s) and the Activation of SARS-CoV-2 Spike (S) Protein |
title | Cryptococcal Protease(s) and the Activation of SARS-CoV-2 Spike (S) Protein |
title_full | Cryptococcal Protease(s) and the Activation of SARS-CoV-2 Spike (S) Protein |
title_fullStr | Cryptococcal Protease(s) and the Activation of SARS-CoV-2 Spike (S) Protein |
title_full_unstemmed | Cryptococcal Protease(s) and the Activation of SARS-CoV-2 Spike (S) Protein |
title_short | Cryptococcal Protease(s) and the Activation of SARS-CoV-2 Spike (S) Protein |
title_sort | cryptococcal protease(s) and the activation of sars-cov-2 spike (s) protein |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834071/ https://www.ncbi.nlm.nih.gov/pubmed/35159253 http://dx.doi.org/10.3390/cells11030437 |
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