Cargando…

Roles of Gonadotropin Receptors in Sexual Development of Medaka

The gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH), are secreted from the pituitary and bind to the FSH receptor (FSHR) and LH receptor (LHR) to regulate gonadal development in vertebrates. Previously, using fshr-knockout (KO) medaka (Oryzias latipes), we demonstrated...

Descripción completa

Detalles Bibliográficos
Autores principales: Kitano, Takeshi, Takenaka, Tomoaki, Takagi, Hisanori, Yoshiura, Yasutoshi, Kazeto, Yukinori, Hirai, Toshiaki, Mukai, Koki, Nozu, Ryo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834109/
https://www.ncbi.nlm.nih.gov/pubmed/35159197
http://dx.doi.org/10.3390/cells11030387
_version_ 1784649101408731136
author Kitano, Takeshi
Takenaka, Tomoaki
Takagi, Hisanori
Yoshiura, Yasutoshi
Kazeto, Yukinori
Hirai, Toshiaki
Mukai, Koki
Nozu, Ryo
author_facet Kitano, Takeshi
Takenaka, Tomoaki
Takagi, Hisanori
Yoshiura, Yasutoshi
Kazeto, Yukinori
Hirai, Toshiaki
Mukai, Koki
Nozu, Ryo
author_sort Kitano, Takeshi
collection PubMed
description The gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH), are secreted from the pituitary and bind to the FSH receptor (FSHR) and LH receptor (LHR) to regulate gonadal development in vertebrates. Previously, using fshr-knockout (KO) medaka (Oryzias latipes), we demonstrated that FSH regulates ovarian development by elevating estrogen levels. However, the lhr-KO phenotype in medaka is poorly characterized. Here, we generated lhr-KO medaka using the transcription activator-like effector nuclease (TALEN) technique. We analyzed its phenotype and that of fshr-KO, lhr;fshr double-heterozygotes (double-hetero), and double-KO fish. All genetically male medaka displayed normal testes and were fertile, whereas fshr-KO and double-KO genetically female fish displayed small ovaries containing many early pre-vitellogenic oocytes and were infertile. Although lhr-KO genetically female fish had normal ovaries with full-grown oocytes, ovulation did not occur. Levels of 17α,20β-dihydroxy-4-pregnen-3-one, which is required for meiotic maturation of oocytes and sperm maturation in teleost fish, were significantly decreased in all KO female medaka ovaries except for double-heteros. Further, 17β-estradiol levels in fshr-KO and double-KO ovaries were significantly lower than those in double-heteros. These findings indicate that LH is necessary for oocyte maturation and FSH is necessary for follicle development, but that neither are essential for spermatogenesis in medaka.
format Online
Article
Text
id pubmed-8834109
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88341092022-02-12 Roles of Gonadotropin Receptors in Sexual Development of Medaka Kitano, Takeshi Takenaka, Tomoaki Takagi, Hisanori Yoshiura, Yasutoshi Kazeto, Yukinori Hirai, Toshiaki Mukai, Koki Nozu, Ryo Cells Article The gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH), are secreted from the pituitary and bind to the FSH receptor (FSHR) and LH receptor (LHR) to regulate gonadal development in vertebrates. Previously, using fshr-knockout (KO) medaka (Oryzias latipes), we demonstrated that FSH regulates ovarian development by elevating estrogen levels. However, the lhr-KO phenotype in medaka is poorly characterized. Here, we generated lhr-KO medaka using the transcription activator-like effector nuclease (TALEN) technique. We analyzed its phenotype and that of fshr-KO, lhr;fshr double-heterozygotes (double-hetero), and double-KO fish. All genetically male medaka displayed normal testes and were fertile, whereas fshr-KO and double-KO genetically female fish displayed small ovaries containing many early pre-vitellogenic oocytes and were infertile. Although lhr-KO genetically female fish had normal ovaries with full-grown oocytes, ovulation did not occur. Levels of 17α,20β-dihydroxy-4-pregnen-3-one, which is required for meiotic maturation of oocytes and sperm maturation in teleost fish, were significantly decreased in all KO female medaka ovaries except for double-heteros. Further, 17β-estradiol levels in fshr-KO and double-KO ovaries were significantly lower than those in double-heteros. These findings indicate that LH is necessary for oocyte maturation and FSH is necessary for follicle development, but that neither are essential for spermatogenesis in medaka. MDPI 2022-01-24 /pmc/articles/PMC8834109/ /pubmed/35159197 http://dx.doi.org/10.3390/cells11030387 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kitano, Takeshi
Takenaka, Tomoaki
Takagi, Hisanori
Yoshiura, Yasutoshi
Kazeto, Yukinori
Hirai, Toshiaki
Mukai, Koki
Nozu, Ryo
Roles of Gonadotropin Receptors in Sexual Development of Medaka
title Roles of Gonadotropin Receptors in Sexual Development of Medaka
title_full Roles of Gonadotropin Receptors in Sexual Development of Medaka
title_fullStr Roles of Gonadotropin Receptors in Sexual Development of Medaka
title_full_unstemmed Roles of Gonadotropin Receptors in Sexual Development of Medaka
title_short Roles of Gonadotropin Receptors in Sexual Development of Medaka
title_sort roles of gonadotropin receptors in sexual development of medaka
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834109/
https://www.ncbi.nlm.nih.gov/pubmed/35159197
http://dx.doi.org/10.3390/cells11030387
work_keys_str_mv AT kitanotakeshi rolesofgonadotropinreceptorsinsexualdevelopmentofmedaka
AT takenakatomoaki rolesofgonadotropinreceptorsinsexualdevelopmentofmedaka
AT takagihisanori rolesofgonadotropinreceptorsinsexualdevelopmentofmedaka
AT yoshiurayasutoshi rolesofgonadotropinreceptorsinsexualdevelopmentofmedaka
AT kazetoyukinori rolesofgonadotropinreceptorsinsexualdevelopmentofmedaka
AT hiraitoshiaki rolesofgonadotropinreceptorsinsexualdevelopmentofmedaka
AT mukaikoki rolesofgonadotropinreceptorsinsexualdevelopmentofmedaka
AT nozuryo rolesofgonadotropinreceptorsinsexualdevelopmentofmedaka