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Testicular Torsion: Preliminary Results of In Vitro Cell Stimulation Using Chorionic Gonadotropin

Testicular torsion is a pathology that occurs in young males generally before the age of 25. Despite surgery representing the only effective approach, there is still a need to identify a marker that can predict whether a preserved testicle will be functional. In addition, no therapeutic approach is...

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Autores principales: Errico, Andrea, Camoglio, Francesco Saverio, Zampieri, Nicola, Dando, Ilaria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834308/
https://www.ncbi.nlm.nih.gov/pubmed/35159259
http://dx.doi.org/10.3390/cells11030450
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author Errico, Andrea
Camoglio, Francesco Saverio
Zampieri, Nicola
Dando, Ilaria
author_facet Errico, Andrea
Camoglio, Francesco Saverio
Zampieri, Nicola
Dando, Ilaria
author_sort Errico, Andrea
collection PubMed
description Testicular torsion is a pathology that occurs in young males generally before the age of 25. Despite surgery representing the only effective approach, there is still a need to identify a marker that can predict whether a preserved testicle will be functional. In addition, no therapeutic approach is currently considered in the post-operative phase. Through an approach based on the in vitro culture of a tissue strictly linked to the testicle, the gubernaculum, we defined the healthy state of the organ and the possible responsiveness to a therapy used in the andrology field, chorionic gonadotropin (hCG). Firstly, we optimized a protocol to obtain viable cells starting from a small piece of gubernacular tissue harvested during surgery with the aim to amplify cells in vitro. Intriguingly, only for a patient whose testicle had been removed during surgery due to an excessive necrotic area, gubernacular cells were not able to grow in culture. These data support the possibility of exploiting the gubernaculum to evaluate the healthy state of the testicle. Then, as we demonstrate that gubernacular cells express a luteinizing hormone receptor, to which hCG is specific, we analyzed the cellular response to hCG treatment on in vitro cultured cells derived from patients affected by testicular torsion. Our study opens the way for the possibility of evaluating testicle wellbeing after derotation through in vitro culture of a small piece of gubernaculum together with predicting the response to the treatment with hCG, which can have a positive effect on cell proliferation and vascularization.
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spelling pubmed-88343082022-02-12 Testicular Torsion: Preliminary Results of In Vitro Cell Stimulation Using Chorionic Gonadotropin Errico, Andrea Camoglio, Francesco Saverio Zampieri, Nicola Dando, Ilaria Cells Commentary Testicular torsion is a pathology that occurs in young males generally before the age of 25. Despite surgery representing the only effective approach, there is still a need to identify a marker that can predict whether a preserved testicle will be functional. In addition, no therapeutic approach is currently considered in the post-operative phase. Through an approach based on the in vitro culture of a tissue strictly linked to the testicle, the gubernaculum, we defined the healthy state of the organ and the possible responsiveness to a therapy used in the andrology field, chorionic gonadotropin (hCG). Firstly, we optimized a protocol to obtain viable cells starting from a small piece of gubernacular tissue harvested during surgery with the aim to amplify cells in vitro. Intriguingly, only for a patient whose testicle had been removed during surgery due to an excessive necrotic area, gubernacular cells were not able to grow in culture. These data support the possibility of exploiting the gubernaculum to evaluate the healthy state of the testicle. Then, as we demonstrate that gubernacular cells express a luteinizing hormone receptor, to which hCG is specific, we analyzed the cellular response to hCG treatment on in vitro cultured cells derived from patients affected by testicular torsion. Our study opens the way for the possibility of evaluating testicle wellbeing after derotation through in vitro culture of a small piece of gubernaculum together with predicting the response to the treatment with hCG, which can have a positive effect on cell proliferation and vascularization. MDPI 2022-01-28 /pmc/articles/PMC8834308/ /pubmed/35159259 http://dx.doi.org/10.3390/cells11030450 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Commentary
Errico, Andrea
Camoglio, Francesco Saverio
Zampieri, Nicola
Dando, Ilaria
Testicular Torsion: Preliminary Results of In Vitro Cell Stimulation Using Chorionic Gonadotropin
title Testicular Torsion: Preliminary Results of In Vitro Cell Stimulation Using Chorionic Gonadotropin
title_full Testicular Torsion: Preliminary Results of In Vitro Cell Stimulation Using Chorionic Gonadotropin
title_fullStr Testicular Torsion: Preliminary Results of In Vitro Cell Stimulation Using Chorionic Gonadotropin
title_full_unstemmed Testicular Torsion: Preliminary Results of In Vitro Cell Stimulation Using Chorionic Gonadotropin
title_short Testicular Torsion: Preliminary Results of In Vitro Cell Stimulation Using Chorionic Gonadotropin
title_sort testicular torsion: preliminary results of in vitro cell stimulation using chorionic gonadotropin
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834308/
https://www.ncbi.nlm.nih.gov/pubmed/35159259
http://dx.doi.org/10.3390/cells11030450
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