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Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome

The circadian clock orchestrates an organism’s endogenous processes with environmental 24 h cycles. Redox homeostasis and the circadian clock regulate one another to negate the potential effects of our planet’s light/dark cycle on the generation of reactive oxygen species (ROS) and attain homeostasi...

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Autores principales: Kay, Holly, Taylor, Harry, van Ooijen, Gerben
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834552/
https://www.ncbi.nlm.nih.gov/pubmed/35159150
http://dx.doi.org/10.3390/cells11030340
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author Kay, Holly
Taylor, Harry
van Ooijen, Gerben
author_facet Kay, Holly
Taylor, Harry
van Ooijen, Gerben
author_sort Kay, Holly
collection PubMed
description The circadian clock orchestrates an organism’s endogenous processes with environmental 24 h cycles. Redox homeostasis and the circadian clock regulate one another to negate the potential effects of our planet’s light/dark cycle on the generation of reactive oxygen species (ROS) and attain homeostasis. Selenoproteins are an important class of redox-related enzymes that have a selenocysteine residue in the active site. This study reports functional understanding of how environmental and endogenous circadian rhythms integrate to shape the selenoproteome in a model eukaryotic cell. We mined quantitative proteomic data for the 24 selenoproteins of the picoeukaryote Ostreococcus tauri across time series, under environmentally rhythmic entrained conditions of light/dark (LD) cycles, compared to constant circadian conditions of constant light (LL). We found an overrepresentation of selenoproteins among rhythmic proteins under LL, but an underrepresentation under LD conditions. Rhythmic selenoproteins under LL that reach peak abundance later in the day showed a greater relative amplitude of oscillations than those that peak early in the day. Under LD, amplitude did not correlate with peak phase; however, we identified high-amplitude selenium uptake rhythms under LD but not LL conditions. Selenium deprivation induced strong qualitative defects in clock gene expression under LD but not LL conditions. Overall, the clear conclusion is that the circadian and environmental cycles exert differential effects on the selenoproteome, and that the combination of the two enables homeostasis. Selenoproteins may therefore play an important role in the cellular response to reactive oxygen species that form as a consequence of the transitions between light and dark.
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spelling pubmed-88345522022-02-12 Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome Kay, Holly Taylor, Harry van Ooijen, Gerben Cells Article The circadian clock orchestrates an organism’s endogenous processes with environmental 24 h cycles. Redox homeostasis and the circadian clock regulate one another to negate the potential effects of our planet’s light/dark cycle on the generation of reactive oxygen species (ROS) and attain homeostasis. Selenoproteins are an important class of redox-related enzymes that have a selenocysteine residue in the active site. This study reports functional understanding of how environmental and endogenous circadian rhythms integrate to shape the selenoproteome in a model eukaryotic cell. We mined quantitative proteomic data for the 24 selenoproteins of the picoeukaryote Ostreococcus tauri across time series, under environmentally rhythmic entrained conditions of light/dark (LD) cycles, compared to constant circadian conditions of constant light (LL). We found an overrepresentation of selenoproteins among rhythmic proteins under LL, but an underrepresentation under LD conditions. Rhythmic selenoproteins under LL that reach peak abundance later in the day showed a greater relative amplitude of oscillations than those that peak early in the day. Under LD, amplitude did not correlate with peak phase; however, we identified high-amplitude selenium uptake rhythms under LD but not LL conditions. Selenium deprivation induced strong qualitative defects in clock gene expression under LD but not LL conditions. Overall, the clear conclusion is that the circadian and environmental cycles exert differential effects on the selenoproteome, and that the combination of the two enables homeostasis. Selenoproteins may therefore play an important role in the cellular response to reactive oxygen species that form as a consequence of the transitions between light and dark. MDPI 2022-01-20 /pmc/articles/PMC8834552/ /pubmed/35159150 http://dx.doi.org/10.3390/cells11030340 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kay, Holly
Taylor, Harry
van Ooijen, Gerben
Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome
title Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome
title_full Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome
title_fullStr Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome
title_full_unstemmed Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome
title_short Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome
title_sort environmental and circadian regulation combine to shape the rhythmic selenoproteome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834552/
https://www.ncbi.nlm.nih.gov/pubmed/35159150
http://dx.doi.org/10.3390/cells11030340
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