Probing the Dynamics of Streptococcus pyogenes Cas9 Endonuclease Bound to the sgRNA Complex Using Hydrogen-Deuterium Exchange Mass Spectrometry

The Cas9 endonuclease is an essential component of the CRISPR–Cas-based genome editing tools. The attainment of high specificity and efficiency of Cas9 during targetted DNA cleavage is the main problem that limits the clinical application of the CRISPR–Cas9 system. A deep understanding of the Cas9 m...

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Detalles Bibliográficos
Autores principales: Zhdanova, Polina V., Chernonosov, Alexander A., Prokhorova, Daria V., Stepanov, Grigory A., Kanazhevskaya, Lyubov Yu., Koval, Vladimir V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834707/
https://www.ncbi.nlm.nih.gov/pubmed/35163047
http://dx.doi.org/10.3390/ijms23031129

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