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Expression Profile of Selected Genes Involved in Storage Lipid Synthesis in a Model Oleaginous Yeast Species Yarrowia lipolytica

Yarrowia lipolytica yeast is a model species of the group of oleaginous microorganisms capable of intracellular lipids accumulation in an amount exceeding 20% of the dry mass. Single cell oil biosynthesis can follow one of two biochemical pathways—de novo accumulation of cellular lipids in medium co...

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Autores principales: Fabiszewska, Agata, Paplińska-Goryca, Magdalena, Misiukiewicz-Stępień, Paulina, Wołoszynowska, Małgorzata, Nowak, Dorota, Zieniuk, Bartłomiej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834811/
https://www.ncbi.nlm.nih.gov/pubmed/35162970
http://dx.doi.org/10.3390/ijms23031041
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author Fabiszewska, Agata
Paplińska-Goryca, Magdalena
Misiukiewicz-Stępień, Paulina
Wołoszynowska, Małgorzata
Nowak, Dorota
Zieniuk, Bartłomiej
author_facet Fabiszewska, Agata
Paplińska-Goryca, Magdalena
Misiukiewicz-Stępień, Paulina
Wołoszynowska, Małgorzata
Nowak, Dorota
Zieniuk, Bartłomiej
author_sort Fabiszewska, Agata
collection PubMed
description Yarrowia lipolytica yeast is a model species of the group of oleaginous microorganisms capable of intracellular lipids accumulation in an amount exceeding 20% of the dry mass. Single cell oil biosynthesis can follow one of two biochemical pathways—de novo accumulation of cellular lipids in medium containing non-lipid carbon sources (including saccharides, glycerol) and ex novo microbial oil synthesis which involves fatty acids uptake from the environment. The mRNA expression of selected genes of de novo and ex novo lipid synthesis pathways was analyzed and correlated with the phenotypically observed features. It was proved that the accumulation yield of storage lipids via ex novo pathway was to some extent dependent on the limitation of the nitrogen source in the medium. It was also proposed that the synthesis of intracellular lipids in lipid-rich medium proceeded mainly via ex novo pathway, although the activity of genes encoding the enzymes of the de novo pathway were not completely inhibited at the stage of transcription by fatty acids present in the medium (e.g., ATP-citrate lyase). Molecular markers of two biosynthesis routes has been outlined and a hypothetical connection point between de novo and ex novo route were indicated.
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spelling pubmed-88348112022-02-12 Expression Profile of Selected Genes Involved in Storage Lipid Synthesis in a Model Oleaginous Yeast Species Yarrowia lipolytica Fabiszewska, Agata Paplińska-Goryca, Magdalena Misiukiewicz-Stępień, Paulina Wołoszynowska, Małgorzata Nowak, Dorota Zieniuk, Bartłomiej Int J Mol Sci Article Yarrowia lipolytica yeast is a model species of the group of oleaginous microorganisms capable of intracellular lipids accumulation in an amount exceeding 20% of the dry mass. Single cell oil biosynthesis can follow one of two biochemical pathways—de novo accumulation of cellular lipids in medium containing non-lipid carbon sources (including saccharides, glycerol) and ex novo microbial oil synthesis which involves fatty acids uptake from the environment. The mRNA expression of selected genes of de novo and ex novo lipid synthesis pathways was analyzed and correlated with the phenotypically observed features. It was proved that the accumulation yield of storage lipids via ex novo pathway was to some extent dependent on the limitation of the nitrogen source in the medium. It was also proposed that the synthesis of intracellular lipids in lipid-rich medium proceeded mainly via ex novo pathway, although the activity of genes encoding the enzymes of the de novo pathway were not completely inhibited at the stage of transcription by fatty acids present in the medium (e.g., ATP-citrate lyase). Molecular markers of two biosynthesis routes has been outlined and a hypothetical connection point between de novo and ex novo route were indicated. MDPI 2022-01-18 /pmc/articles/PMC8834811/ /pubmed/35162970 http://dx.doi.org/10.3390/ijms23031041 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fabiszewska, Agata
Paplińska-Goryca, Magdalena
Misiukiewicz-Stępień, Paulina
Wołoszynowska, Małgorzata
Nowak, Dorota
Zieniuk, Bartłomiej
Expression Profile of Selected Genes Involved in Storage Lipid Synthesis in a Model Oleaginous Yeast Species Yarrowia lipolytica
title Expression Profile of Selected Genes Involved in Storage Lipid Synthesis in a Model Oleaginous Yeast Species Yarrowia lipolytica
title_full Expression Profile of Selected Genes Involved in Storage Lipid Synthesis in a Model Oleaginous Yeast Species Yarrowia lipolytica
title_fullStr Expression Profile of Selected Genes Involved in Storage Lipid Synthesis in a Model Oleaginous Yeast Species Yarrowia lipolytica
title_full_unstemmed Expression Profile of Selected Genes Involved in Storage Lipid Synthesis in a Model Oleaginous Yeast Species Yarrowia lipolytica
title_short Expression Profile of Selected Genes Involved in Storage Lipid Synthesis in a Model Oleaginous Yeast Species Yarrowia lipolytica
title_sort expression profile of selected genes involved in storage lipid synthesis in a model oleaginous yeast species yarrowia lipolytica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834811/
https://www.ncbi.nlm.nih.gov/pubmed/35162970
http://dx.doi.org/10.3390/ijms23031041
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