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Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software

In this study, we evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories during a multicenter networking validation study. The stud...

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Autores principales: Gato, Eva, Anantharajah, Ahalieyah, Arroyo, Manuel J., Artacho, María José, Caballero, Juan de Dios, Candela, Ana, Chudějová, Kateřina, Constanso, Ignacio Pedro, Elías, Cristina, Fernández, Javier, Jiménez, Jesús, Lumbreras, Pilar, Méndez, Gema, Mulet, Xavier, Pérez-Palacios, Patricia, Rodríguez-Sánchez, Belén, Cantón, Rafael, Hrabák, Jaroslav, Mancera, Luis, Martínez-Martínez, Luis, Oliver, Antonio, Pascual, Álvaro, Verroken, Alexia, Bou, Germán, Oviaño, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834885/
https://www.ncbi.nlm.nih.gov/pubmed/35154029
http://dx.doi.org/10.3389/fmicb.2021.789731
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author Gato, Eva
Anantharajah, Ahalieyah
Arroyo, Manuel J.
Artacho, María José
Caballero, Juan de Dios
Candela, Ana
Chudějová, Kateřina
Constanso, Ignacio Pedro
Elías, Cristina
Fernández, Javier
Jiménez, Jesús
Lumbreras, Pilar
Méndez, Gema
Mulet, Xavier
Pérez-Palacios, Patricia
Rodríguez-Sánchez, Belén
Cantón, Rafael
Hrabák, Jaroslav
Mancera, Luis
Martínez-Martínez, Luis
Oliver, Antonio
Pascual, Álvaro
Verroken, Alexia
Bou, Germán
Oviaño, Marina
author_facet Gato, Eva
Anantharajah, Ahalieyah
Arroyo, Manuel J.
Artacho, María José
Caballero, Juan de Dios
Candela, Ana
Chudějová, Kateřina
Constanso, Ignacio Pedro
Elías, Cristina
Fernández, Javier
Jiménez, Jesús
Lumbreras, Pilar
Méndez, Gema
Mulet, Xavier
Pérez-Palacios, Patricia
Rodríguez-Sánchez, Belén
Cantón, Rafael
Hrabák, Jaroslav
Mancera, Luis
Martínez-Martínez, Luis
Oliver, Antonio
Pascual, Álvaro
Verroken, Alexia
Bou, Germán
Oviaño, Marina
author_sort Gato, Eva
collection PubMed
description In this study, we evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories during a multicenter networking validation study. The study was divided into three different stages: “software design,” “intercenter evaluation,” and “clinical validation.” First, a standardized procedure with an online software for data analysis was designed. Carbapenem resistance was detected by measuring imipenem hydrolysis and the results were automatically interpreted using the Clover MS data analysis software (Clover BioSoft, Spain). Second, a series of 74 genotypically characterized Enterobacterales (46 carbapenemase-producers and 28 non carbapenemase-producers) were analyzed in 8 international centers to ensure the reproducibility of the method. Finally, the methodology was evaluated independently in all centers during a 2-month period and results were compared with the reference standard for carbapenemase detection used in each center. The overall agreement rate relative to the reference method for carbapenemase resistance detection in clinical samples was 92.5%. The sensitivity was 93.9% and the specificity, 100%. Results were obtained within 60 min and accuracy ranged from 83.3 to 100% among the different centers. Further, our results demonstrate that MALDI-TOF MS is an outstanding tool for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories. The use of a simple in-house procedure with online software allows routine screening of carbapenemases in diagnostics, thereby facilitating early and appropriate antimicrobial therapy.
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spelling pubmed-88348852022-02-12 Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software Gato, Eva Anantharajah, Ahalieyah Arroyo, Manuel J. Artacho, María José Caballero, Juan de Dios Candela, Ana Chudějová, Kateřina Constanso, Ignacio Pedro Elías, Cristina Fernández, Javier Jiménez, Jesús Lumbreras, Pilar Méndez, Gema Mulet, Xavier Pérez-Palacios, Patricia Rodríguez-Sánchez, Belén Cantón, Rafael Hrabák, Jaroslav Mancera, Luis Martínez-Martínez, Luis Oliver, Antonio Pascual, Álvaro Verroken, Alexia Bou, Germán Oviaño, Marina Front Microbiol Microbiology In this study, we evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories during a multicenter networking validation study. The study was divided into three different stages: “software design,” “intercenter evaluation,” and “clinical validation.” First, a standardized procedure with an online software for data analysis was designed. Carbapenem resistance was detected by measuring imipenem hydrolysis and the results were automatically interpreted using the Clover MS data analysis software (Clover BioSoft, Spain). Second, a series of 74 genotypically characterized Enterobacterales (46 carbapenemase-producers and 28 non carbapenemase-producers) were analyzed in 8 international centers to ensure the reproducibility of the method. Finally, the methodology was evaluated independently in all centers during a 2-month period and results were compared with the reference standard for carbapenemase detection used in each center. The overall agreement rate relative to the reference method for carbapenemase resistance detection in clinical samples was 92.5%. The sensitivity was 93.9% and the specificity, 100%. Results were obtained within 60 min and accuracy ranged from 83.3 to 100% among the different centers. Further, our results demonstrate that MALDI-TOF MS is an outstanding tool for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories. The use of a simple in-house procedure with online software allows routine screening of carbapenemases in diagnostics, thereby facilitating early and appropriate antimicrobial therapy. Frontiers Media S.A. 2022-01-27 /pmc/articles/PMC8834885/ /pubmed/35154029 http://dx.doi.org/10.3389/fmicb.2021.789731 Text en Copyright © 2022 Gato, Anantharajah, Arroyo, Artacho, Caballero, Candela, Chudějová, Constanso, Elías, Fernández, Jiménez, Lumbreras, Méndez, Mulet, Pérez-Palacios, Rodríguez-Sánchez, Cantón, Hrabák, Mancera, Martínez-Martínez, Oliver, Pascual, Verroken, Bou and Oviaño. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gato, Eva
Anantharajah, Ahalieyah
Arroyo, Manuel J.
Artacho, María José
Caballero, Juan de Dios
Candela, Ana
Chudějová, Kateřina
Constanso, Ignacio Pedro
Elías, Cristina
Fernández, Javier
Jiménez, Jesús
Lumbreras, Pilar
Méndez, Gema
Mulet, Xavier
Pérez-Palacios, Patricia
Rodríguez-Sánchez, Belén
Cantón, Rafael
Hrabák, Jaroslav
Mancera, Luis
Martínez-Martínez, Luis
Oliver, Antonio
Pascual, Álvaro
Verroken, Alexia
Bou, Germán
Oviaño, Marina
Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software
title Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software
title_full Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software
title_fullStr Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software
title_full_unstemmed Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software
title_short Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software
title_sort multicenter performance evaluation of maldi-tof ms for rapid detection of carbapenemase activity in enterobacterales: the future of networking data analysis with online software
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834885/
https://www.ncbi.nlm.nih.gov/pubmed/35154029
http://dx.doi.org/10.3389/fmicb.2021.789731
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