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Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software
In this study, we evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories during a multicenter networking validation study. The stud...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834885/ https://www.ncbi.nlm.nih.gov/pubmed/35154029 http://dx.doi.org/10.3389/fmicb.2021.789731 |
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author | Gato, Eva Anantharajah, Ahalieyah Arroyo, Manuel J. Artacho, María José Caballero, Juan de Dios Candela, Ana Chudějová, Kateřina Constanso, Ignacio Pedro Elías, Cristina Fernández, Javier Jiménez, Jesús Lumbreras, Pilar Méndez, Gema Mulet, Xavier Pérez-Palacios, Patricia Rodríguez-Sánchez, Belén Cantón, Rafael Hrabák, Jaroslav Mancera, Luis Martínez-Martínez, Luis Oliver, Antonio Pascual, Álvaro Verroken, Alexia Bou, Germán Oviaño, Marina |
author_facet | Gato, Eva Anantharajah, Ahalieyah Arroyo, Manuel J. Artacho, María José Caballero, Juan de Dios Candela, Ana Chudějová, Kateřina Constanso, Ignacio Pedro Elías, Cristina Fernández, Javier Jiménez, Jesús Lumbreras, Pilar Méndez, Gema Mulet, Xavier Pérez-Palacios, Patricia Rodríguez-Sánchez, Belén Cantón, Rafael Hrabák, Jaroslav Mancera, Luis Martínez-Martínez, Luis Oliver, Antonio Pascual, Álvaro Verroken, Alexia Bou, Germán Oviaño, Marina |
author_sort | Gato, Eva |
collection | PubMed |
description | In this study, we evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories during a multicenter networking validation study. The study was divided into three different stages: “software design,” “intercenter evaluation,” and “clinical validation.” First, a standardized procedure with an online software for data analysis was designed. Carbapenem resistance was detected by measuring imipenem hydrolysis and the results were automatically interpreted using the Clover MS data analysis software (Clover BioSoft, Spain). Second, a series of 74 genotypically characterized Enterobacterales (46 carbapenemase-producers and 28 non carbapenemase-producers) were analyzed in 8 international centers to ensure the reproducibility of the method. Finally, the methodology was evaluated independently in all centers during a 2-month period and results were compared with the reference standard for carbapenemase detection used in each center. The overall agreement rate relative to the reference method for carbapenemase resistance detection in clinical samples was 92.5%. The sensitivity was 93.9% and the specificity, 100%. Results were obtained within 60 min and accuracy ranged from 83.3 to 100% among the different centers. Further, our results demonstrate that MALDI-TOF MS is an outstanding tool for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories. The use of a simple in-house procedure with online software allows routine screening of carbapenemases in diagnostics, thereby facilitating early and appropriate antimicrobial therapy. |
format | Online Article Text |
id | pubmed-8834885 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88348852022-02-12 Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software Gato, Eva Anantharajah, Ahalieyah Arroyo, Manuel J. Artacho, María José Caballero, Juan de Dios Candela, Ana Chudějová, Kateřina Constanso, Ignacio Pedro Elías, Cristina Fernández, Javier Jiménez, Jesús Lumbreras, Pilar Méndez, Gema Mulet, Xavier Pérez-Palacios, Patricia Rodríguez-Sánchez, Belén Cantón, Rafael Hrabák, Jaroslav Mancera, Luis Martínez-Martínez, Luis Oliver, Antonio Pascual, Álvaro Verroken, Alexia Bou, Germán Oviaño, Marina Front Microbiol Microbiology In this study, we evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories during a multicenter networking validation study. The study was divided into three different stages: “software design,” “intercenter evaluation,” and “clinical validation.” First, a standardized procedure with an online software for data analysis was designed. Carbapenem resistance was detected by measuring imipenem hydrolysis and the results were automatically interpreted using the Clover MS data analysis software (Clover BioSoft, Spain). Second, a series of 74 genotypically characterized Enterobacterales (46 carbapenemase-producers and 28 non carbapenemase-producers) were analyzed in 8 international centers to ensure the reproducibility of the method. Finally, the methodology was evaluated independently in all centers during a 2-month period and results were compared with the reference standard for carbapenemase detection used in each center. The overall agreement rate relative to the reference method for carbapenemase resistance detection in clinical samples was 92.5%. The sensitivity was 93.9% and the specificity, 100%. Results were obtained within 60 min and accuracy ranged from 83.3 to 100% among the different centers. Further, our results demonstrate that MALDI-TOF MS is an outstanding tool for rapid detection of carbapenemase activity in Enterobacterales in clinical microbiology laboratories. The use of a simple in-house procedure with online software allows routine screening of carbapenemases in diagnostics, thereby facilitating early and appropriate antimicrobial therapy. Frontiers Media S.A. 2022-01-27 /pmc/articles/PMC8834885/ /pubmed/35154029 http://dx.doi.org/10.3389/fmicb.2021.789731 Text en Copyright © 2022 Gato, Anantharajah, Arroyo, Artacho, Caballero, Candela, Chudějová, Constanso, Elías, Fernández, Jiménez, Lumbreras, Méndez, Mulet, Pérez-Palacios, Rodríguez-Sánchez, Cantón, Hrabák, Mancera, Martínez-Martínez, Oliver, Pascual, Verroken, Bou and Oviaño. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Gato, Eva Anantharajah, Ahalieyah Arroyo, Manuel J. Artacho, María José Caballero, Juan de Dios Candela, Ana Chudějová, Kateřina Constanso, Ignacio Pedro Elías, Cristina Fernández, Javier Jiménez, Jesús Lumbreras, Pilar Méndez, Gema Mulet, Xavier Pérez-Palacios, Patricia Rodríguez-Sánchez, Belén Cantón, Rafael Hrabák, Jaroslav Mancera, Luis Martínez-Martínez, Luis Oliver, Antonio Pascual, Álvaro Verroken, Alexia Bou, Germán Oviaño, Marina Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
title | Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
title_full | Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
title_fullStr | Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
title_full_unstemmed | Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
title_short | Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The Future of Networking Data Analysis With Online Software |
title_sort | multicenter performance evaluation of maldi-tof ms for rapid detection of carbapenemase activity in enterobacterales: the future of networking data analysis with online software |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8834885/ https://www.ncbi.nlm.nih.gov/pubmed/35154029 http://dx.doi.org/10.3389/fmicb.2021.789731 |
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