Cargando…

Photodynamic Activity of Protoporphyrin IX-Immobilized Cellulose Monolith for Nerve Tissue Regeneration

The development of nerve conduits with a three-dimensional porous structure has attracted great attention as they closely mimic the major features of the natural extracellular matrix of the nerve tissue. As low levels of reactive oxygen species (ROS) function as signaling molecules to promote cell p...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Ji Hye, Kim, Ki Hong, Kwon, Oh Hyeong, Kwon, Oh Kyoung, Uyama, Hiroshi, Kim, Young-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835291/
https://www.ncbi.nlm.nih.gov/pubmed/35162958
http://dx.doi.org/10.3390/ijms23031035
_version_ 1784649396125696000
author Lee, Ji Hye
Kim, Ki Hong
Kwon, Oh Hyeong
Kwon, Oh Kyoung
Uyama, Hiroshi
Kim, Young-Jin
author_facet Lee, Ji Hye
Kim, Ki Hong
Kwon, Oh Hyeong
Kwon, Oh Kyoung
Uyama, Hiroshi
Kim, Young-Jin
author_sort Lee, Ji Hye
collection PubMed
description The development of nerve conduits with a three-dimensional porous structure has attracted great attention as they closely mimic the major features of the natural extracellular matrix of the nerve tissue. As low levels of reactive oxygen species (ROS) function as signaling molecules to promote cell proliferation and growth, this study aimed to fabricate protoporphyrin IX (PpIX)-immobilized cellulose (CEPP) monoliths as a means to both guide and stimulate nerve regeneration. CEPP monoliths can be fabricated via a simple thermally induced phase separation method and surface modification. The improved nerve tissue regeneration of CEPP monoliths was achieved by the activation of mitogen-activated protein kinases, such as extracellular signal-regulated kinases (ERKs). The resulting CEPP monoliths exhibited interconnected microporous structures and uniform morphology. The results of in vitro bioactivity assays demonstrated that the CEPP monoliths with under 0.54 ± 0.07 μmol/g PpIX exhibited enhanced photodynamic activity on Schwann cells via the generation of low levels of ROS. This photodynamic activation of the CEPP monoliths is a cell-safe process to stimulate cell proliferation without cytotoxic side effects. In addition, the protein expression of phospho-ERK increased considerably after the laser irradiation on the CEPP monoliths with low content of PpIX. Therefore, the CEPP monoliths have a potential application in nerve tissue regeneration as new nerve conduits.
format Online
Article
Text
id pubmed-8835291
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88352912022-02-12 Photodynamic Activity of Protoporphyrin IX-Immobilized Cellulose Monolith for Nerve Tissue Regeneration Lee, Ji Hye Kim, Ki Hong Kwon, Oh Hyeong Kwon, Oh Kyoung Uyama, Hiroshi Kim, Young-Jin Int J Mol Sci Article The development of nerve conduits with a three-dimensional porous structure has attracted great attention as they closely mimic the major features of the natural extracellular matrix of the nerve tissue. As low levels of reactive oxygen species (ROS) function as signaling molecules to promote cell proliferation and growth, this study aimed to fabricate protoporphyrin IX (PpIX)-immobilized cellulose (CEPP) monoliths as a means to both guide and stimulate nerve regeneration. CEPP monoliths can be fabricated via a simple thermally induced phase separation method and surface modification. The improved nerve tissue regeneration of CEPP monoliths was achieved by the activation of mitogen-activated protein kinases, such as extracellular signal-regulated kinases (ERKs). The resulting CEPP monoliths exhibited interconnected microporous structures and uniform morphology. The results of in vitro bioactivity assays demonstrated that the CEPP monoliths with under 0.54 ± 0.07 μmol/g PpIX exhibited enhanced photodynamic activity on Schwann cells via the generation of low levels of ROS. This photodynamic activation of the CEPP monoliths is a cell-safe process to stimulate cell proliferation without cytotoxic side effects. In addition, the protein expression of phospho-ERK increased considerably after the laser irradiation on the CEPP monoliths with low content of PpIX. Therefore, the CEPP monoliths have a potential application in nerve tissue regeneration as new nerve conduits. MDPI 2022-01-18 /pmc/articles/PMC8835291/ /pubmed/35162958 http://dx.doi.org/10.3390/ijms23031035 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Ji Hye
Kim, Ki Hong
Kwon, Oh Hyeong
Kwon, Oh Kyoung
Uyama, Hiroshi
Kim, Young-Jin
Photodynamic Activity of Protoporphyrin IX-Immobilized Cellulose Monolith for Nerve Tissue Regeneration
title Photodynamic Activity of Protoporphyrin IX-Immobilized Cellulose Monolith for Nerve Tissue Regeneration
title_full Photodynamic Activity of Protoporphyrin IX-Immobilized Cellulose Monolith for Nerve Tissue Regeneration
title_fullStr Photodynamic Activity of Protoporphyrin IX-Immobilized Cellulose Monolith for Nerve Tissue Regeneration
title_full_unstemmed Photodynamic Activity of Protoporphyrin IX-Immobilized Cellulose Monolith for Nerve Tissue Regeneration
title_short Photodynamic Activity of Protoporphyrin IX-Immobilized Cellulose Monolith for Nerve Tissue Regeneration
title_sort photodynamic activity of protoporphyrin ix-immobilized cellulose monolith for nerve tissue regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835291/
https://www.ncbi.nlm.nih.gov/pubmed/35162958
http://dx.doi.org/10.3390/ijms23031035
work_keys_str_mv AT leejihye photodynamicactivityofprotoporphyriniximmobilizedcellulosemonolithfornervetissueregeneration
AT kimkihong photodynamicactivityofprotoporphyriniximmobilizedcellulosemonolithfornervetissueregeneration
AT kwonohhyeong photodynamicactivityofprotoporphyriniximmobilizedcellulosemonolithfornervetissueregeneration
AT kwonohkyoung photodynamicactivityofprotoporphyriniximmobilizedcellulosemonolithfornervetissueregeneration
AT uyamahiroshi photodynamicactivityofprotoporphyriniximmobilizedcellulosemonolithfornervetissueregeneration
AT kimyoungjin photodynamicactivityofprotoporphyriniximmobilizedcellulosemonolithfornervetissueregeneration