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E8002 Reduces Adhesion Formation and Improves Joint Mobility in a Rat Model of Knee Arthrofibrosis
Knee arthrofibrosis is a common complication of knee surgery, caused by excessive scar tissue, which results in functional disability. However, no curative treatment has been established. E8002 is an anti-adhesion material that contains L-ascorbic acid, an antioxidant. We aimed to evaluate the effic...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835358/ https://www.ncbi.nlm.nih.gov/pubmed/35163163 http://dx.doi.org/10.3390/ijms23031239 |
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author | Takada, Seiya Setoyama, Kentaro Norimatsu, Kosuke Otsuka, Shotaro Nakanishi, Kazuki Tani, Akira Nakakogawa, Tomomi Matsuzaki, Ryoma Matsuoka, Teruki Sakakima, Harutoshi Tancharoen, Salunya Maruyama, Ikuro Tanaka, Eiichiro Kikuchi, Kiyoshi Uchikado, Hisaaki |
author_facet | Takada, Seiya Setoyama, Kentaro Norimatsu, Kosuke Otsuka, Shotaro Nakanishi, Kazuki Tani, Akira Nakakogawa, Tomomi Matsuzaki, Ryoma Matsuoka, Teruki Sakakima, Harutoshi Tancharoen, Salunya Maruyama, Ikuro Tanaka, Eiichiro Kikuchi, Kiyoshi Uchikado, Hisaaki |
author_sort | Takada, Seiya |
collection | PubMed |
description | Knee arthrofibrosis is a common complication of knee surgery, caused by excessive scar tissue, which results in functional disability. However, no curative treatment has been established. E8002 is an anti-adhesion material that contains L-ascorbic acid, an antioxidant. We aimed to evaluate the efficacy of E8002 for the prevention of knee arthrofibrosis in a rat model, comprising injury to the surface of the femur and quadriceps muscle 1 cm proximal to the patella. Sixteen male, 8-week-old Sprague Dawley rats were studied: in the Adhesion group, haemorrhagic injury was induced to the quadriceps and bone, and in the E8002 group, an adhesion-preventing film was implanted between the quadriceps and femur after injury. Six weeks following injury, the restriction of knee flexion owing to fibrotic scarring had not worsened in the E8002 group but had worsened in the Adhesion group. The area of fibrotic scarring was smaller in the E8002 group than in the Adhesion group (p < 0.05). In addition, the numbers of fibroblasts (p < 0.05) and myofibroblasts (p < 0.01) in the fibrotic scar were lower in the E8002 group. Thus, E8002 reduces myofibroblast proliferation and fibrotic scar formation and improves the range of motion of the joint in a model of knee injury. |
format | Online Article Text |
id | pubmed-8835358 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88353582022-02-12 E8002 Reduces Adhesion Formation and Improves Joint Mobility in a Rat Model of Knee Arthrofibrosis Takada, Seiya Setoyama, Kentaro Norimatsu, Kosuke Otsuka, Shotaro Nakanishi, Kazuki Tani, Akira Nakakogawa, Tomomi Matsuzaki, Ryoma Matsuoka, Teruki Sakakima, Harutoshi Tancharoen, Salunya Maruyama, Ikuro Tanaka, Eiichiro Kikuchi, Kiyoshi Uchikado, Hisaaki Int J Mol Sci Article Knee arthrofibrosis is a common complication of knee surgery, caused by excessive scar tissue, which results in functional disability. However, no curative treatment has been established. E8002 is an anti-adhesion material that contains L-ascorbic acid, an antioxidant. We aimed to evaluate the efficacy of E8002 for the prevention of knee arthrofibrosis in a rat model, comprising injury to the surface of the femur and quadriceps muscle 1 cm proximal to the patella. Sixteen male, 8-week-old Sprague Dawley rats were studied: in the Adhesion group, haemorrhagic injury was induced to the quadriceps and bone, and in the E8002 group, an adhesion-preventing film was implanted between the quadriceps and femur after injury. Six weeks following injury, the restriction of knee flexion owing to fibrotic scarring had not worsened in the E8002 group but had worsened in the Adhesion group. The area of fibrotic scarring was smaller in the E8002 group than in the Adhesion group (p < 0.05). In addition, the numbers of fibroblasts (p < 0.05) and myofibroblasts (p < 0.01) in the fibrotic scar were lower in the E8002 group. Thus, E8002 reduces myofibroblast proliferation and fibrotic scar formation and improves the range of motion of the joint in a model of knee injury. MDPI 2022-01-22 /pmc/articles/PMC8835358/ /pubmed/35163163 http://dx.doi.org/10.3390/ijms23031239 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Takada, Seiya Setoyama, Kentaro Norimatsu, Kosuke Otsuka, Shotaro Nakanishi, Kazuki Tani, Akira Nakakogawa, Tomomi Matsuzaki, Ryoma Matsuoka, Teruki Sakakima, Harutoshi Tancharoen, Salunya Maruyama, Ikuro Tanaka, Eiichiro Kikuchi, Kiyoshi Uchikado, Hisaaki E8002 Reduces Adhesion Formation and Improves Joint Mobility in a Rat Model of Knee Arthrofibrosis |
title | E8002 Reduces Adhesion Formation and Improves Joint Mobility in a Rat Model of Knee Arthrofibrosis |
title_full | E8002 Reduces Adhesion Formation and Improves Joint Mobility in a Rat Model of Knee Arthrofibrosis |
title_fullStr | E8002 Reduces Adhesion Formation and Improves Joint Mobility in a Rat Model of Knee Arthrofibrosis |
title_full_unstemmed | E8002 Reduces Adhesion Formation and Improves Joint Mobility in a Rat Model of Knee Arthrofibrosis |
title_short | E8002 Reduces Adhesion Formation and Improves Joint Mobility in a Rat Model of Knee Arthrofibrosis |
title_sort | e8002 reduces adhesion formation and improves joint mobility in a rat model of knee arthrofibrosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835358/ https://www.ncbi.nlm.nih.gov/pubmed/35163163 http://dx.doi.org/10.3390/ijms23031239 |
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