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Combined Modification of Fiber Materials by Enzymes and Metal Nanoparticles for Chemical and Biological Protection
To obtain fiber materials with pronounced chemical-biological protection, metal (Zn or Ta) nanoparticles were jointly applied with polyelectrolyte complexes of enzymes and polypeptides being their stabilizers. Computer modeling revealed the preferences between certain polyelectrolyte partners for N-...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835833/ https://www.ncbi.nlm.nih.gov/pubmed/35163283 http://dx.doi.org/10.3390/ijms23031359 |
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author | Lyagin, Ilya Stepanov, Nikolay Frolov, George Efremenko, Elena |
author_facet | Lyagin, Ilya Stepanov, Nikolay Frolov, George Efremenko, Elena |
author_sort | Lyagin, Ilya |
collection | PubMed |
description | To obtain fiber materials with pronounced chemical-biological protection, metal (Zn or Ta) nanoparticles were jointly applied with polyelectrolyte complexes of enzymes and polypeptides being their stabilizers. Computer modeling revealed the preferences between certain polyelectrolyte partners for N-acyl-homoserine lactone acylase and hexahistidine-tagged organophosphorus hydrolase (His(6)-OPH) possessing the quorum quenching (QQ) behavior with bacterial cells. The combinations of metal nanoparticles and enzymes appeared to function better as compared to the combinations of the same QQ-enzymes with antibiotics (polymyxins), making it possible to decrease the applied quantities by orders of magnitude while giving the same effect. The elimination of Gram-positive and Gram-negative bacterial cells from doubly modified fiber materials notably increased (up to 2.9-fold), whereas His(6)-OPH retained its hydrolytic activity in reaction with organophosphorus compounds (up to 74% of initially applied activity). Materials with the certain enzyme and Zn nanoparticles were more efficient against Bacillus subtilis cells (up to 2.1-fold), and Ta nanoparticles acted preferentially against Escherichia coli (up to 1.5-fold). Some materials were proved to be more suitable for combined modification by metal nanoparticles and His(6)-OPH complexes as antimicrobial protectants. |
format | Online Article Text |
id | pubmed-8835833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88358332022-02-12 Combined Modification of Fiber Materials by Enzymes and Metal Nanoparticles for Chemical and Biological Protection Lyagin, Ilya Stepanov, Nikolay Frolov, George Efremenko, Elena Int J Mol Sci Article To obtain fiber materials with pronounced chemical-biological protection, metal (Zn or Ta) nanoparticles were jointly applied with polyelectrolyte complexes of enzymes and polypeptides being their stabilizers. Computer modeling revealed the preferences between certain polyelectrolyte partners for N-acyl-homoserine lactone acylase and hexahistidine-tagged organophosphorus hydrolase (His(6)-OPH) possessing the quorum quenching (QQ) behavior with bacterial cells. The combinations of metal nanoparticles and enzymes appeared to function better as compared to the combinations of the same QQ-enzymes with antibiotics (polymyxins), making it possible to decrease the applied quantities by orders of magnitude while giving the same effect. The elimination of Gram-positive and Gram-negative bacterial cells from doubly modified fiber materials notably increased (up to 2.9-fold), whereas His(6)-OPH retained its hydrolytic activity in reaction with organophosphorus compounds (up to 74% of initially applied activity). Materials with the certain enzyme and Zn nanoparticles were more efficient against Bacillus subtilis cells (up to 2.1-fold), and Ta nanoparticles acted preferentially against Escherichia coli (up to 1.5-fold). Some materials were proved to be more suitable for combined modification by metal nanoparticles and His(6)-OPH complexes as antimicrobial protectants. MDPI 2022-01-25 /pmc/articles/PMC8835833/ /pubmed/35163283 http://dx.doi.org/10.3390/ijms23031359 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lyagin, Ilya Stepanov, Nikolay Frolov, George Efremenko, Elena Combined Modification of Fiber Materials by Enzymes and Metal Nanoparticles for Chemical and Biological Protection |
title | Combined Modification of Fiber Materials by Enzymes and Metal Nanoparticles for Chemical and Biological Protection |
title_full | Combined Modification of Fiber Materials by Enzymes and Metal Nanoparticles for Chemical and Biological Protection |
title_fullStr | Combined Modification of Fiber Materials by Enzymes and Metal Nanoparticles for Chemical and Biological Protection |
title_full_unstemmed | Combined Modification of Fiber Materials by Enzymes and Metal Nanoparticles for Chemical and Biological Protection |
title_short | Combined Modification of Fiber Materials by Enzymes and Metal Nanoparticles for Chemical and Biological Protection |
title_sort | combined modification of fiber materials by enzymes and metal nanoparticles for chemical and biological protection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835833/ https://www.ncbi.nlm.nih.gov/pubmed/35163283 http://dx.doi.org/10.3390/ijms23031359 |
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