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Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm
Optical microscopy has vastly expanded the frontiers of structural and functional biology, due to the non-invasive probing of dynamic volumes in vivo. However, traditional widefield microscopy illuminating the entire field of view (FOV) is adversely affected by out-of-focus light scatter. Consequent...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835835/ https://www.ncbi.nlm.nih.gov/pubmed/35163562 http://dx.doi.org/10.3390/ijms23031643 |
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author | Teranikar, Tanveer Lim, Jessica Ijaseun, Toluwani Lee, Juhyun |
author_facet | Teranikar, Tanveer Lim, Jessica Ijaseun, Toluwani Lee, Juhyun |
author_sort | Teranikar, Tanveer |
collection | PubMed |
description | Optical microscopy has vastly expanded the frontiers of structural and functional biology, due to the non-invasive probing of dynamic volumes in vivo. However, traditional widefield microscopy illuminating the entire field of view (FOV) is adversely affected by out-of-focus light scatter. Consequently, standard upright or inverted microscopes are inept in sampling diffraction-limited volumes smaller than the optical system’s point spread function (PSF). Over the last few decades, several planar and structured (sinusoidal) illumination modalities have offered unprecedented access to sub-cellular organelles and 4D (3D + time) image acquisition. Furthermore, these optical sectioning systems remain unaffected by the size of biological samples, providing high signal-to-noise (SNR) ratios for objective lenses (OLs) with long working distances (WDs). This review aims to guide biologists regarding planar illumination strategies, capable of harnessing sub-micron spatial resolution with a millimeter depth of penetration. |
format | Online Article Text |
id | pubmed-8835835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88358352022-02-12 Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm Teranikar, Tanveer Lim, Jessica Ijaseun, Toluwani Lee, Juhyun Int J Mol Sci Review Optical microscopy has vastly expanded the frontiers of structural and functional biology, due to the non-invasive probing of dynamic volumes in vivo. However, traditional widefield microscopy illuminating the entire field of view (FOV) is adversely affected by out-of-focus light scatter. Consequently, standard upright or inverted microscopes are inept in sampling diffraction-limited volumes smaller than the optical system’s point spread function (PSF). Over the last few decades, several planar and structured (sinusoidal) illumination modalities have offered unprecedented access to sub-cellular organelles and 4D (3D + time) image acquisition. Furthermore, these optical sectioning systems remain unaffected by the size of biological samples, providing high signal-to-noise (SNR) ratios for objective lenses (OLs) with long working distances (WDs). This review aims to guide biologists regarding planar illumination strategies, capable of harnessing sub-micron spatial resolution with a millimeter depth of penetration. MDPI 2022-01-31 /pmc/articles/PMC8835835/ /pubmed/35163562 http://dx.doi.org/10.3390/ijms23031643 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Teranikar, Tanveer Lim, Jessica Ijaseun, Toluwani Lee, Juhyun Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm |
title | Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm |
title_full | Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm |
title_fullStr | Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm |
title_full_unstemmed | Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm |
title_short | Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm |
title_sort | development of planar illumination strategies for solving mysteries in the sub-cellular realm |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835835/ https://www.ncbi.nlm.nih.gov/pubmed/35163562 http://dx.doi.org/10.3390/ijms23031643 |
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