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Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm

Optical microscopy has vastly expanded the frontiers of structural and functional biology, due to the non-invasive probing of dynamic volumes in vivo. However, traditional widefield microscopy illuminating the entire field of view (FOV) is adversely affected by out-of-focus light scatter. Consequent...

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Detalles Bibliográficos
Autores principales: Teranikar, Tanveer, Lim, Jessica, Ijaseun, Toluwani, Lee, Juhyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835835/
https://www.ncbi.nlm.nih.gov/pubmed/35163562
http://dx.doi.org/10.3390/ijms23031643
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author Teranikar, Tanveer
Lim, Jessica
Ijaseun, Toluwani
Lee, Juhyun
author_facet Teranikar, Tanveer
Lim, Jessica
Ijaseun, Toluwani
Lee, Juhyun
author_sort Teranikar, Tanveer
collection PubMed
description Optical microscopy has vastly expanded the frontiers of structural and functional biology, due to the non-invasive probing of dynamic volumes in vivo. However, traditional widefield microscopy illuminating the entire field of view (FOV) is adversely affected by out-of-focus light scatter. Consequently, standard upright or inverted microscopes are inept in sampling diffraction-limited volumes smaller than the optical system’s point spread function (PSF). Over the last few decades, several planar and structured (sinusoidal) illumination modalities have offered unprecedented access to sub-cellular organelles and 4D (3D + time) image acquisition. Furthermore, these optical sectioning systems remain unaffected by the size of biological samples, providing high signal-to-noise (SNR) ratios for objective lenses (OLs) with long working distances (WDs). This review aims to guide biologists regarding planar illumination strategies, capable of harnessing sub-micron spatial resolution with a millimeter depth of penetration.
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spelling pubmed-88358352022-02-12 Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm Teranikar, Tanveer Lim, Jessica Ijaseun, Toluwani Lee, Juhyun Int J Mol Sci Review Optical microscopy has vastly expanded the frontiers of structural and functional biology, due to the non-invasive probing of dynamic volumes in vivo. However, traditional widefield microscopy illuminating the entire field of view (FOV) is adversely affected by out-of-focus light scatter. Consequently, standard upright or inverted microscopes are inept in sampling diffraction-limited volumes smaller than the optical system’s point spread function (PSF). Over the last few decades, several planar and structured (sinusoidal) illumination modalities have offered unprecedented access to sub-cellular organelles and 4D (3D + time) image acquisition. Furthermore, these optical sectioning systems remain unaffected by the size of biological samples, providing high signal-to-noise (SNR) ratios for objective lenses (OLs) with long working distances (WDs). This review aims to guide biologists regarding planar illumination strategies, capable of harnessing sub-micron spatial resolution with a millimeter depth of penetration. MDPI 2022-01-31 /pmc/articles/PMC8835835/ /pubmed/35163562 http://dx.doi.org/10.3390/ijms23031643 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Teranikar, Tanveer
Lim, Jessica
Ijaseun, Toluwani
Lee, Juhyun
Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm
title Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm
title_full Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm
title_fullStr Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm
title_full_unstemmed Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm
title_short Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm
title_sort development of planar illumination strategies for solving mysteries in the sub-cellular realm
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835835/
https://www.ncbi.nlm.nih.gov/pubmed/35163562
http://dx.doi.org/10.3390/ijms23031643
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