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Incorporation of a FRET Pair into a Riboswitch RNA to Measure Mg(2+) Concentration and RNA Conformational Change in Cell
Riboswitches are natural biosensors that can regulate gene expression by sensing small molecules. Knowledge of the structural dynamics of riboswitches is crucial to elucidate their regulatory mechanism and develop RNA biosensors. In this work, we incorporated the fluorophore, Cy3, and its quencher,...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MDPI
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835884/ https://www.ncbi.nlm.nih.gov/pubmed/35163416 http://dx.doi.org/10.3390/ijms23031493 |