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Anti-Obesity Efficacy of Pediococcus acidilactici MNL5 in Canorhabditis elegans Gut Model

In the present study, thirty two lactic acid bacteria (LAB) were isolated from fermented Indian herbal medicine. In comparison to other strains, MNL5 had stronger bile salt hydrolase (BSH) and cholesterol-lowering properties. Furthermore, it can withstand the extreme conditions found in the GI tract...

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Autores principales: Barathikannan, Kaliyan, Chelliah, Ramachandran, Elahi, Fazle, Tyagi, Akanksha, Selvakumar, Vijayalakshmi, Agastian, Paul, Valan Arasu, Mariadhas, Oh, Deog-Hawn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835910/
https://www.ncbi.nlm.nih.gov/pubmed/35163199
http://dx.doi.org/10.3390/ijms23031276
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author Barathikannan, Kaliyan
Chelliah, Ramachandran
Elahi, Fazle
Tyagi, Akanksha
Selvakumar, Vijayalakshmi
Agastian, Paul
Valan Arasu, Mariadhas
Oh, Deog-Hawn
author_facet Barathikannan, Kaliyan
Chelliah, Ramachandran
Elahi, Fazle
Tyagi, Akanksha
Selvakumar, Vijayalakshmi
Agastian, Paul
Valan Arasu, Mariadhas
Oh, Deog-Hawn
author_sort Barathikannan, Kaliyan
collection PubMed
description In the present study, thirty two lactic acid bacteria (LAB) were isolated from fermented Indian herbal medicine. In comparison to other strains, MNL5 had stronger bile salt hydrolase (BSH) and cholesterol-lowering properties. Furthermore, it can withstand the extreme conditions found in the GI tract, due to, e.g., pepsin, bile salts, pancreatin, and acids. Pediococcus acidilactici MNL5 was identified as a probiotic candidate after sequencing the 16S rRNA gene. The antibacterial activity of P. acidilactici MNL5 cell-free supernatants (CFS) against Escherichia coli, Staphylococcus aureus, Helicobacter pylori, Bacillus cereus, and Candida albicans was moderate. A Caenorhabditis elegans experiment was also performed to assess the effectiveness of P. acidilactici MNL5 supplementation to increase life span compared to E. coli supplementation (DAF-2 and LIU1 models) (p < 0.05). An immense reduction of the lipid droplets of C. elegans was identified through a fluorescent microscope. The drastic alteration of the expression of fat genes is related to obesity phenotypes. Hence, several paths are evolutionary for C. elegans; the results of our work highlight the nematode as an important model for obesity.
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spelling pubmed-88359102022-02-12 Anti-Obesity Efficacy of Pediococcus acidilactici MNL5 in Canorhabditis elegans Gut Model Barathikannan, Kaliyan Chelliah, Ramachandran Elahi, Fazle Tyagi, Akanksha Selvakumar, Vijayalakshmi Agastian, Paul Valan Arasu, Mariadhas Oh, Deog-Hawn Int J Mol Sci Article In the present study, thirty two lactic acid bacteria (LAB) were isolated from fermented Indian herbal medicine. In comparison to other strains, MNL5 had stronger bile salt hydrolase (BSH) and cholesterol-lowering properties. Furthermore, it can withstand the extreme conditions found in the GI tract, due to, e.g., pepsin, bile salts, pancreatin, and acids. Pediococcus acidilactici MNL5 was identified as a probiotic candidate after sequencing the 16S rRNA gene. The antibacterial activity of P. acidilactici MNL5 cell-free supernatants (CFS) against Escherichia coli, Staphylococcus aureus, Helicobacter pylori, Bacillus cereus, and Candida albicans was moderate. A Caenorhabditis elegans experiment was also performed to assess the effectiveness of P. acidilactici MNL5 supplementation to increase life span compared to E. coli supplementation (DAF-2 and LIU1 models) (p < 0.05). An immense reduction of the lipid droplets of C. elegans was identified through a fluorescent microscope. The drastic alteration of the expression of fat genes is related to obesity phenotypes. Hence, several paths are evolutionary for C. elegans; the results of our work highlight the nematode as an important model for obesity. MDPI 2022-01-24 /pmc/articles/PMC8835910/ /pubmed/35163199 http://dx.doi.org/10.3390/ijms23031276 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Barathikannan, Kaliyan
Chelliah, Ramachandran
Elahi, Fazle
Tyagi, Akanksha
Selvakumar, Vijayalakshmi
Agastian, Paul
Valan Arasu, Mariadhas
Oh, Deog-Hawn
Anti-Obesity Efficacy of Pediococcus acidilactici MNL5 in Canorhabditis elegans Gut Model
title Anti-Obesity Efficacy of Pediococcus acidilactici MNL5 in Canorhabditis elegans Gut Model
title_full Anti-Obesity Efficacy of Pediococcus acidilactici MNL5 in Canorhabditis elegans Gut Model
title_fullStr Anti-Obesity Efficacy of Pediococcus acidilactici MNL5 in Canorhabditis elegans Gut Model
title_full_unstemmed Anti-Obesity Efficacy of Pediococcus acidilactici MNL5 in Canorhabditis elegans Gut Model
title_short Anti-Obesity Efficacy of Pediococcus acidilactici MNL5 in Canorhabditis elegans Gut Model
title_sort anti-obesity efficacy of pediococcus acidilactici mnl5 in canorhabditis elegans gut model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835910/
https://www.ncbi.nlm.nih.gov/pubmed/35163199
http://dx.doi.org/10.3390/ijms23031276
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