Cargando…
Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis
Cold stress can significantly affect the development, yield, and quality of crops and restrict the geographical distribution and growing seasons of plants. Aquaporins are the main channels for water transport in plant cells. Abiotic stresses such as cold and drought dehydrate cells by changing the w...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836057/ https://www.ncbi.nlm.nih.gov/pubmed/35163287 http://dx.doi.org/10.3390/ijms23031361 |
_version_ | 1784649583227305984 |
---|---|
author | Cheng, Gongmin Wang, Mengdi Zhang, Longyan Wei, Hengling Wang, Hantao Lu, Jianhua Yu, Shuxun |
author_facet | Cheng, Gongmin Wang, Mengdi Zhang, Longyan Wei, Hengling Wang, Hantao Lu, Jianhua Yu, Shuxun |
author_sort | Cheng, Gongmin |
collection | PubMed |
description | Cold stress can significantly affect the development, yield, and quality of crops and restrict the geographical distribution and growing seasons of plants. Aquaporins are the main channels for water transport in plant cells. Abiotic stresses such as cold and drought dehydrate cells by changing the water potential. In this study, we cloned a gene GhTIP1;1-like encodes tonoplast aquaporin from the transcriptome database of cotton seedlings after cold stress. Expression analysis showed that GhTIP1;1-like not only responds to cold stress but was also induced by heat, drought and salt stress. Subcellular localization showed that the protein was anchored to the vacuole membrane. Promoter deletion analysis revealed that a MYC motif within the promoter region of GhTIP1;1-like were the core cis-elements in response to low temperature. Virus-induced gene silencing (VIGS) and histochemical staining indicate that GhTIP1;1-like plays a positive role in plant cold tolerance. Overexpression of GhTIP1;1-like in Arabidopsis delayed the senescence process and enhanced the cold tolerance of transgenic plants. Compared with the wild type, the soluble protein concentration and peroxidase activity of the transgenic lines under cold stress were higher, while the malondialdehyde content was lower. In addition, the expression levels of cold-responsive genes were significantly increased in transgenic plants under cold stress. Our results indicate that GhTIP1;1-like could respond to different abiotic stresses and be positively involved in regulating the cold tolerance of cotton. |
format | Online Article Text |
id | pubmed-8836057 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88360572022-02-12 Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis Cheng, Gongmin Wang, Mengdi Zhang, Longyan Wei, Hengling Wang, Hantao Lu, Jianhua Yu, Shuxun Int J Mol Sci Article Cold stress can significantly affect the development, yield, and quality of crops and restrict the geographical distribution and growing seasons of plants. Aquaporins are the main channels for water transport in plant cells. Abiotic stresses such as cold and drought dehydrate cells by changing the water potential. In this study, we cloned a gene GhTIP1;1-like encodes tonoplast aquaporin from the transcriptome database of cotton seedlings after cold stress. Expression analysis showed that GhTIP1;1-like not only responds to cold stress but was also induced by heat, drought and salt stress. Subcellular localization showed that the protein was anchored to the vacuole membrane. Promoter deletion analysis revealed that a MYC motif within the promoter region of GhTIP1;1-like were the core cis-elements in response to low temperature. Virus-induced gene silencing (VIGS) and histochemical staining indicate that GhTIP1;1-like plays a positive role in plant cold tolerance. Overexpression of GhTIP1;1-like in Arabidopsis delayed the senescence process and enhanced the cold tolerance of transgenic plants. Compared with the wild type, the soluble protein concentration and peroxidase activity of the transgenic lines under cold stress were higher, while the malondialdehyde content was lower. In addition, the expression levels of cold-responsive genes were significantly increased in transgenic plants under cold stress. Our results indicate that GhTIP1;1-like could respond to different abiotic stresses and be positively involved in regulating the cold tolerance of cotton. MDPI 2022-01-25 /pmc/articles/PMC8836057/ /pubmed/35163287 http://dx.doi.org/10.3390/ijms23031361 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cheng, Gongmin Wang, Mengdi Zhang, Longyan Wei, Hengling Wang, Hantao Lu, Jianhua Yu, Shuxun Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis |
title | Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis |
title_full | Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis |
title_fullStr | Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis |
title_full_unstemmed | Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis |
title_short | Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis |
title_sort | overexpression of a cotton aquaporin gene ghtip1;1-like confers cold tolerance in transgenic arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836057/ https://www.ncbi.nlm.nih.gov/pubmed/35163287 http://dx.doi.org/10.3390/ijms23031361 |
work_keys_str_mv | AT chenggongmin overexpressionofacottonaquaporingeneghtip11likeconferscoldtoleranceintransgenicarabidopsis AT wangmengdi overexpressionofacottonaquaporingeneghtip11likeconferscoldtoleranceintransgenicarabidopsis AT zhanglongyan overexpressionofacottonaquaporingeneghtip11likeconferscoldtoleranceintransgenicarabidopsis AT weihengling overexpressionofacottonaquaporingeneghtip11likeconferscoldtoleranceintransgenicarabidopsis AT wanghantao overexpressionofacottonaquaporingeneghtip11likeconferscoldtoleranceintransgenicarabidopsis AT lujianhua overexpressionofacottonaquaporingeneghtip11likeconferscoldtoleranceintransgenicarabidopsis AT yushuxun overexpressionofacottonaquaporingeneghtip11likeconferscoldtoleranceintransgenicarabidopsis |