A Review of the Advantages, Disadvantages and Limitations of Chemotaxis Assays for Campylobacter spp.

Reproducible qualitative and quantitative assessment of bacterial chemotactic motility, particularly in response to chemorepellent effectors, is experimentally challenging. Here we compare several established chemotaxis assays currently used to investigate Campylobacter jejuni chemotaxis, with the a...

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Detalles Bibliográficos
Autores principales: Elgamoudi, Bassam A., Korolik, Victoria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836060/
https://www.ncbi.nlm.nih.gov/pubmed/35163499
http://dx.doi.org/10.3390/ijms23031576
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author Elgamoudi, Bassam A.
Korolik, Victoria
author_facet Elgamoudi, Bassam A.
Korolik, Victoria
author_sort Elgamoudi, Bassam A.
collection PubMed
description Reproducible qualitative and quantitative assessment of bacterial chemotactic motility, particularly in response to chemorepellent effectors, is experimentally challenging. Here we compare several established chemotaxis assays currently used to investigate Campylobacter jejuni chemotaxis, with the aim of improving the correlation between different studies and establishing the best practices. We compare the methodologies of capillary, agar, and chamber-based assays, and discuss critical technical points, in terms of reproducibility, accuracy, and the advantages and limitations of each.
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spelling pubmed-88360602022-02-12 A Review of the Advantages, Disadvantages and Limitations of Chemotaxis Assays for Campylobacter spp. Elgamoudi, Bassam A. Korolik, Victoria Int J Mol Sci Review Reproducible qualitative and quantitative assessment of bacterial chemotactic motility, particularly in response to chemorepellent effectors, is experimentally challenging. Here we compare several established chemotaxis assays currently used to investigate Campylobacter jejuni chemotaxis, with the aim of improving the correlation between different studies and establishing the best practices. We compare the methodologies of capillary, agar, and chamber-based assays, and discuss critical technical points, in terms of reproducibility, accuracy, and the advantages and limitations of each. MDPI 2022-01-29 /pmc/articles/PMC8836060/ /pubmed/35163499 http://dx.doi.org/10.3390/ijms23031576 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Elgamoudi, Bassam A.
Korolik, Victoria
A Review of the Advantages, Disadvantages and Limitations of Chemotaxis Assays for Campylobacter spp.
title A Review of the Advantages, Disadvantages and Limitations of Chemotaxis Assays for Campylobacter spp.
title_full A Review of the Advantages, Disadvantages and Limitations of Chemotaxis Assays for Campylobacter spp.
title_fullStr A Review of the Advantages, Disadvantages and Limitations of Chemotaxis Assays for Campylobacter spp.
title_full_unstemmed A Review of the Advantages, Disadvantages and Limitations of Chemotaxis Assays for Campylobacter spp.
title_short A Review of the Advantages, Disadvantages and Limitations of Chemotaxis Assays for Campylobacter spp.
title_sort review of the advantages, disadvantages and limitations of chemotaxis assays for campylobacter spp.
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836060/
https://www.ncbi.nlm.nih.gov/pubmed/35163499
http://dx.doi.org/10.3390/ijms23031576
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