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Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells
Activity-based monitoring of cell-secreted proteases has gained significant interest due to the implication of these substances in diverse cellular functions. Here, we demonstrated a cell-based method of monitoring protease activity using fluorescent cell-permeable peptides. The activatable peptide...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836073/ https://www.ncbi.nlm.nih.gov/pubmed/35163529 http://dx.doi.org/10.3390/ijms23031605 |
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author | Kim, Gae-Baik Lee, Jeong Min Nguyen, Duc Long Lee, Joonseok Kim, Young-Pil |
author_facet | Kim, Gae-Baik Lee, Jeong Min Nguyen, Duc Long Lee, Joonseok Kim, Young-Pil |
author_sort | Kim, Gae-Baik |
collection | PubMed |
description | Activity-based monitoring of cell-secreted proteases has gained significant interest due to the implication of these substances in diverse cellular functions. Here, we demonstrated a cell-based method of monitoring protease activity using fluorescent cell-permeable peptides. The activatable peptide consists of anionic (EEEE), cleavable, and cationic sequences (RRRR) that enable intracellular delivery by matrix metalloproteinase-2 (MMP2), which is secreted by living cancer cells. Compared to HT-29 cells (MMP2-negative), HT-1080 cells (MMP2-positive) showed a strong fluorescence response to the short fluorescent peptide via cell-secreted protease activation. Our approach is expected to find applications for the rapid visualization of protease activity in living cells. |
format | Online Article Text |
id | pubmed-8836073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88360732022-02-12 Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells Kim, Gae-Baik Lee, Jeong Min Nguyen, Duc Long Lee, Joonseok Kim, Young-Pil Int J Mol Sci Article Activity-based monitoring of cell-secreted proteases has gained significant interest due to the implication of these substances in diverse cellular functions. Here, we demonstrated a cell-based method of monitoring protease activity using fluorescent cell-permeable peptides. The activatable peptide consists of anionic (EEEE), cleavable, and cationic sequences (RRRR) that enable intracellular delivery by matrix metalloproteinase-2 (MMP2), which is secreted by living cancer cells. Compared to HT-29 cells (MMP2-negative), HT-1080 cells (MMP2-positive) showed a strong fluorescence response to the short fluorescent peptide via cell-secreted protease activation. Our approach is expected to find applications for the rapid visualization of protease activity in living cells. MDPI 2022-01-30 /pmc/articles/PMC8836073/ /pubmed/35163529 http://dx.doi.org/10.3390/ijms23031605 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Gae-Baik Lee, Jeong Min Nguyen, Duc Long Lee, Joonseok Kim, Young-Pil Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells |
title | Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells |
title_full | Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells |
title_fullStr | Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells |
title_full_unstemmed | Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells |
title_short | Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells |
title_sort | activatable peptides for rapid and simple visualization of protease activity secreted in living cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836073/ https://www.ncbi.nlm.nih.gov/pubmed/35163529 http://dx.doi.org/10.3390/ijms23031605 |
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