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Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars

Rice crops are often subject to multiple abiotic stresses simultaneously in both natural and cultivated environments, resulting in yield reductions beyond those expected from single stress. We report physiological changes after a 4 day exposure to combined drought, salt and extreme temperature treat...

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Autores principales: Habibpourmehraban, Fatemeh, Wu, Yunqi, Wu, Jemma X., Hamzelou, Sara, Masoomi-Aladizgeh, Farhad, Kamath, Karthik Shantharam, Amirkhani, Ardeshir, Atwell, Brian J., Haynes, Paul A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836074/
https://www.ncbi.nlm.nih.gov/pubmed/35163659
http://dx.doi.org/10.3390/ijms23031739
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author Habibpourmehraban, Fatemeh
Wu, Yunqi
Wu, Jemma X.
Hamzelou, Sara
Masoomi-Aladizgeh, Farhad
Kamath, Karthik Shantharam
Amirkhani, Ardeshir
Atwell, Brian J.
Haynes, Paul A.
author_facet Habibpourmehraban, Fatemeh
Wu, Yunqi
Wu, Jemma X.
Hamzelou, Sara
Masoomi-Aladizgeh, Farhad
Kamath, Karthik Shantharam
Amirkhani, Ardeshir
Atwell, Brian J.
Haynes, Paul A.
author_sort Habibpourmehraban, Fatemeh
collection PubMed
description Rice crops are often subject to multiple abiotic stresses simultaneously in both natural and cultivated environments, resulting in yield reductions beyond those expected from single stress. We report physiological changes after a 4 day exposure to combined drought, salt and extreme temperature treatments, following a 2 day salinity pre-treatment in two rice genotypes—Nipponbare (a paddy rice) and IAC1131 (an upland landrace). Stomata closed after two days of combined stresses, causing intercellular CO2 concentrations and assimilation rates to diminish rapidly. Abscisic acid (ABA) levels increased at least five-fold but did not differ significantly between the genotypes. Tandem Mass Tag isotopic labelling quantitative proteomics revealed 6215 reproducibly identified proteins in mature leaves across the two genotypes and three time points (0, 2 and 4 days of stress). Of these, 987 were differentially expressed due to stress (cf. control plants), including 41 proteins that changed significantly in abundance in all stressed plants. Heat shock proteins, late embryogenesis abundant proteins and photosynthesis-related proteins were consistently responsive to stress in both Nipponbare and IAC1131. Remarkably, even after 2 days of stress there were almost six times fewer proteins differentially expressed in IAC1131 than Nipponbare. This contrast in the translational response to multiple stresses is consistent with the known tolerance of IAC1131 to dryland conditions.
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spelling pubmed-88360742022-02-12 Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars Habibpourmehraban, Fatemeh Wu, Yunqi Wu, Jemma X. Hamzelou, Sara Masoomi-Aladizgeh, Farhad Kamath, Karthik Shantharam Amirkhani, Ardeshir Atwell, Brian J. Haynes, Paul A. Int J Mol Sci Article Rice crops are often subject to multiple abiotic stresses simultaneously in both natural and cultivated environments, resulting in yield reductions beyond those expected from single stress. We report physiological changes after a 4 day exposure to combined drought, salt and extreme temperature treatments, following a 2 day salinity pre-treatment in two rice genotypes—Nipponbare (a paddy rice) and IAC1131 (an upland landrace). Stomata closed after two days of combined stresses, causing intercellular CO2 concentrations and assimilation rates to diminish rapidly. Abscisic acid (ABA) levels increased at least five-fold but did not differ significantly between the genotypes. Tandem Mass Tag isotopic labelling quantitative proteomics revealed 6215 reproducibly identified proteins in mature leaves across the two genotypes and three time points (0, 2 and 4 days of stress). Of these, 987 were differentially expressed due to stress (cf. control plants), including 41 proteins that changed significantly in abundance in all stressed plants. Heat shock proteins, late embryogenesis abundant proteins and photosynthesis-related proteins were consistently responsive to stress in both Nipponbare and IAC1131. Remarkably, even after 2 days of stress there were almost six times fewer proteins differentially expressed in IAC1131 than Nipponbare. This contrast in the translational response to multiple stresses is consistent with the known tolerance of IAC1131 to dryland conditions. MDPI 2022-02-03 /pmc/articles/PMC8836074/ /pubmed/35163659 http://dx.doi.org/10.3390/ijms23031739 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Habibpourmehraban, Fatemeh
Wu, Yunqi
Wu, Jemma X.
Hamzelou, Sara
Masoomi-Aladizgeh, Farhad
Kamath, Karthik Shantharam
Amirkhani, Ardeshir
Atwell, Brian J.
Haynes, Paul A.
Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars
title Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars
title_full Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars
title_fullStr Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars
title_full_unstemmed Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars
title_short Multiple Abiotic Stresses Applied Simultaneously Elicit Distinct Responses in Two Contrasting Rice Cultivars
title_sort multiple abiotic stresses applied simultaneously elicit distinct responses in two contrasting rice cultivars
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836074/
https://www.ncbi.nlm.nih.gov/pubmed/35163659
http://dx.doi.org/10.3390/ijms23031739
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