Cargando…

Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives

Cytogenetic approaches play an essential role as a quick evaluation of the first genetic effects after mutagenic treatment. Although labor-intensive and time-consuming, they are essential for the analyses of cytotoxic and genotoxic effects in mutagenesis and environmental monitoring. Over the years,...

Descripción completa

Detalles Bibliográficos
Autores principales: Kwasniewska, Jolanta, Bara, Adrianna Wiktoria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836153/
https://www.ncbi.nlm.nih.gov/pubmed/35163228
http://dx.doi.org/10.3390/ijms23031306
_version_ 1784649606989086720
author Kwasniewska, Jolanta
Bara, Adrianna Wiktoria
author_facet Kwasniewska, Jolanta
Bara, Adrianna Wiktoria
author_sort Kwasniewska, Jolanta
collection PubMed
description Cytogenetic approaches play an essential role as a quick evaluation of the first genetic effects after mutagenic treatment. Although labor-intensive and time-consuming, they are essential for the analyses of cytotoxic and genotoxic effects in mutagenesis and environmental monitoring. Over the years, conventional cytogenetic analyses were a part of routine laboratory testing in plant genotoxicity. Among the methods that are used to study genotoxicity in plants, the micronucleus test particularly represents a significant force. Currently, cytogenetic techniques go beyond the simple detection of chromosome aberrations. The intensive development of molecular biology and the significantly improved microscopic visualization and evaluation methods constituted significant support to traditional cytogenetics. Over the past years, distinct approaches have allowed an understanding the mechanisms of formation, structure, and genetic activity of the micronuclei. Although there are many studies on this topic in humans and animals, knowledge in plants is significantly limited. This article provides a comprehensive overview of the current knowledge on micronuclei characteristics in plants. We pay particular attention to how the recent contemporary achievements have influenced the understanding of micronuclei in plant cells. Together with the current progress, we present the latest applications of the micronucleus test in mutagenesis and assess the state of the environment.
format Online
Article
Text
id pubmed-8836153
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88361532022-02-12 Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives Kwasniewska, Jolanta Bara, Adrianna Wiktoria Int J Mol Sci Review Cytogenetic approaches play an essential role as a quick evaluation of the first genetic effects after mutagenic treatment. Although labor-intensive and time-consuming, they are essential for the analyses of cytotoxic and genotoxic effects in mutagenesis and environmental monitoring. Over the years, conventional cytogenetic analyses were a part of routine laboratory testing in plant genotoxicity. Among the methods that are used to study genotoxicity in plants, the micronucleus test particularly represents a significant force. Currently, cytogenetic techniques go beyond the simple detection of chromosome aberrations. The intensive development of molecular biology and the significantly improved microscopic visualization and evaluation methods constituted significant support to traditional cytogenetics. Over the past years, distinct approaches have allowed an understanding the mechanisms of formation, structure, and genetic activity of the micronuclei. Although there are many studies on this topic in humans and animals, knowledge in plants is significantly limited. This article provides a comprehensive overview of the current knowledge on micronuclei characteristics in plants. We pay particular attention to how the recent contemporary achievements have influenced the understanding of micronuclei in plant cells. Together with the current progress, we present the latest applications of the micronucleus test in mutagenesis and assess the state of the environment. MDPI 2022-01-24 /pmc/articles/PMC8836153/ /pubmed/35163228 http://dx.doi.org/10.3390/ijms23031306 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Kwasniewska, Jolanta
Bara, Adrianna Wiktoria
Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives
title Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives
title_full Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives
title_fullStr Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives
title_full_unstemmed Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives
title_short Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives
title_sort plant cytogenetics in the micronuclei investigation—the past, current status, and perspectives
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836153/
https://www.ncbi.nlm.nih.gov/pubmed/35163228
http://dx.doi.org/10.3390/ijms23031306
work_keys_str_mv AT kwasniewskajolanta plantcytogeneticsinthemicronucleiinvestigationthepastcurrentstatusandperspectives
AT baraadriannawiktoria plantcytogeneticsinthemicronucleiinvestigationthepastcurrentstatusandperspectives