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Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives
Cytogenetic approaches play an essential role as a quick evaluation of the first genetic effects after mutagenic treatment. Although labor-intensive and time-consuming, they are essential for the analyses of cytotoxic and genotoxic effects in mutagenesis and environmental monitoring. Over the years,...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836153/ https://www.ncbi.nlm.nih.gov/pubmed/35163228 http://dx.doi.org/10.3390/ijms23031306 |
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author | Kwasniewska, Jolanta Bara, Adrianna Wiktoria |
author_facet | Kwasniewska, Jolanta Bara, Adrianna Wiktoria |
author_sort | Kwasniewska, Jolanta |
collection | PubMed |
description | Cytogenetic approaches play an essential role as a quick evaluation of the first genetic effects after mutagenic treatment. Although labor-intensive and time-consuming, they are essential for the analyses of cytotoxic and genotoxic effects in mutagenesis and environmental monitoring. Over the years, conventional cytogenetic analyses were a part of routine laboratory testing in plant genotoxicity. Among the methods that are used to study genotoxicity in plants, the micronucleus test particularly represents a significant force. Currently, cytogenetic techniques go beyond the simple detection of chromosome aberrations. The intensive development of molecular biology and the significantly improved microscopic visualization and evaluation methods constituted significant support to traditional cytogenetics. Over the past years, distinct approaches have allowed an understanding the mechanisms of formation, structure, and genetic activity of the micronuclei. Although there are many studies on this topic in humans and animals, knowledge in plants is significantly limited. This article provides a comprehensive overview of the current knowledge on micronuclei characteristics in plants. We pay particular attention to how the recent contemporary achievements have influenced the understanding of micronuclei in plant cells. Together with the current progress, we present the latest applications of the micronucleus test in mutagenesis and assess the state of the environment. |
format | Online Article Text |
id | pubmed-8836153 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88361532022-02-12 Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives Kwasniewska, Jolanta Bara, Adrianna Wiktoria Int J Mol Sci Review Cytogenetic approaches play an essential role as a quick evaluation of the first genetic effects after mutagenic treatment. Although labor-intensive and time-consuming, they are essential for the analyses of cytotoxic and genotoxic effects in mutagenesis and environmental monitoring. Over the years, conventional cytogenetic analyses were a part of routine laboratory testing in plant genotoxicity. Among the methods that are used to study genotoxicity in plants, the micronucleus test particularly represents a significant force. Currently, cytogenetic techniques go beyond the simple detection of chromosome aberrations. The intensive development of molecular biology and the significantly improved microscopic visualization and evaluation methods constituted significant support to traditional cytogenetics. Over the past years, distinct approaches have allowed an understanding the mechanisms of formation, structure, and genetic activity of the micronuclei. Although there are many studies on this topic in humans and animals, knowledge in plants is significantly limited. This article provides a comprehensive overview of the current knowledge on micronuclei characteristics in plants. We pay particular attention to how the recent contemporary achievements have influenced the understanding of micronuclei in plant cells. Together with the current progress, we present the latest applications of the micronucleus test in mutagenesis and assess the state of the environment. MDPI 2022-01-24 /pmc/articles/PMC8836153/ /pubmed/35163228 http://dx.doi.org/10.3390/ijms23031306 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Kwasniewska, Jolanta Bara, Adrianna Wiktoria Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives |
title | Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives |
title_full | Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives |
title_fullStr | Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives |
title_full_unstemmed | Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives |
title_short | Plant Cytogenetics in the Micronuclei Investigation—The Past, Current Status, and Perspectives |
title_sort | plant cytogenetics in the micronuclei investigation—the past, current status, and perspectives |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836153/ https://www.ncbi.nlm.nih.gov/pubmed/35163228 http://dx.doi.org/10.3390/ijms23031306 |
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