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3D Printed Graphene-PLA Scaffolds Promote Cell Alignment and Differentiation

Traumas and chronic damages can hamper the regenerative power of nervous, muscle, and connective tissues. Tissue engineering approaches are promising therapeutic tools, aiming to develop reliable, reproducible, and economically affordable synthetic scaffolds which could provide sufficient biomimetic...

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Autores principales: Gasparotto, Matteo, Bellet, Pietro, Scapin, Giorgia, Busetto, Rebecca, Rampazzo, Chiara, Vitiello, Libero, Shah, Dhvanit Indravadan, Filippini, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836229/
https://www.ncbi.nlm.nih.gov/pubmed/35163657
http://dx.doi.org/10.3390/ijms23031736
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author Gasparotto, Matteo
Bellet, Pietro
Scapin, Giorgia
Busetto, Rebecca
Rampazzo, Chiara
Vitiello, Libero
Shah, Dhvanit Indravadan
Filippini, Francesco
author_facet Gasparotto, Matteo
Bellet, Pietro
Scapin, Giorgia
Busetto, Rebecca
Rampazzo, Chiara
Vitiello, Libero
Shah, Dhvanit Indravadan
Filippini, Francesco
author_sort Gasparotto, Matteo
collection PubMed
description Traumas and chronic damages can hamper the regenerative power of nervous, muscle, and connective tissues. Tissue engineering approaches are promising therapeutic tools, aiming to develop reliable, reproducible, and economically affordable synthetic scaffolds which could provide sufficient biomimetic cues to promote the desired cell behaviour without triggering graft rejection and transplant failure. Here, we used 3D-printing to develop 3D-printed scaffolds based on either PLA or graphene@PLA with a defined pattern. Multiple regeneration strategies require a specific orientation of implanted and recruited cells to perform their function correctly. We tested our scaffolds with induced pluripotent stem cells (iPSC), neuronal-like cells, immortalised fibroblasts and myoblasts. Our results demonstrated that the specific “lines and ridges” 100 µm-scaffold topography is sufficient to promote myoblast and fibroblast cell alignment and orient neurites along with the scaffolds line pattern. Conversely, graphene is critical to promote cells differentiation, as seen by the iPSC commitment to neuroectoderm, and myoblast fusions into multinuclear myotubes achieved by the 100 µm scaffolds containing graphene. This work shows the development of a reliable and economical 3D-printed scaffold with the potential of being used in multiple tissue engineering applications and elucidates how scaffold micro-topography and graphene properties synergistically control cell differentiation.
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spelling pubmed-88362292022-02-12 3D Printed Graphene-PLA Scaffolds Promote Cell Alignment and Differentiation Gasparotto, Matteo Bellet, Pietro Scapin, Giorgia Busetto, Rebecca Rampazzo, Chiara Vitiello, Libero Shah, Dhvanit Indravadan Filippini, Francesco Int J Mol Sci Article Traumas and chronic damages can hamper the regenerative power of nervous, muscle, and connective tissues. Tissue engineering approaches are promising therapeutic tools, aiming to develop reliable, reproducible, and economically affordable synthetic scaffolds which could provide sufficient biomimetic cues to promote the desired cell behaviour without triggering graft rejection and transplant failure. Here, we used 3D-printing to develop 3D-printed scaffolds based on either PLA or graphene@PLA with a defined pattern. Multiple regeneration strategies require a specific orientation of implanted and recruited cells to perform their function correctly. We tested our scaffolds with induced pluripotent stem cells (iPSC), neuronal-like cells, immortalised fibroblasts and myoblasts. Our results demonstrated that the specific “lines and ridges” 100 µm-scaffold topography is sufficient to promote myoblast and fibroblast cell alignment and orient neurites along with the scaffolds line pattern. Conversely, graphene is critical to promote cells differentiation, as seen by the iPSC commitment to neuroectoderm, and myoblast fusions into multinuclear myotubes achieved by the 100 µm scaffolds containing graphene. This work shows the development of a reliable and economical 3D-printed scaffold with the potential of being used in multiple tissue engineering applications and elucidates how scaffold micro-topography and graphene properties synergistically control cell differentiation. MDPI 2022-02-03 /pmc/articles/PMC8836229/ /pubmed/35163657 http://dx.doi.org/10.3390/ijms23031736 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gasparotto, Matteo
Bellet, Pietro
Scapin, Giorgia
Busetto, Rebecca
Rampazzo, Chiara
Vitiello, Libero
Shah, Dhvanit Indravadan
Filippini, Francesco
3D Printed Graphene-PLA Scaffolds Promote Cell Alignment and Differentiation
title 3D Printed Graphene-PLA Scaffolds Promote Cell Alignment and Differentiation
title_full 3D Printed Graphene-PLA Scaffolds Promote Cell Alignment and Differentiation
title_fullStr 3D Printed Graphene-PLA Scaffolds Promote Cell Alignment and Differentiation
title_full_unstemmed 3D Printed Graphene-PLA Scaffolds Promote Cell Alignment and Differentiation
title_short 3D Printed Graphene-PLA Scaffolds Promote Cell Alignment and Differentiation
title_sort 3d printed graphene-pla scaffolds promote cell alignment and differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836229/
https://www.ncbi.nlm.nih.gov/pubmed/35163657
http://dx.doi.org/10.3390/ijms23031736
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