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Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes

The use of silver nanoparticles (Ag NPs) in the biomedical field deserves a mindful analysis of the possible inflammatory response which could limit their use in the clinic. Despite the anti-cancer properties of Ag NPs having been widely demonstrated, there are still few studies concerning their inv...

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Autores principales: Cascione, Mariafrancesca, Rizzello, Loris, Manno, Daniela, Serra, Antonio, De Matteis, Valeria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836503/
https://www.ncbi.nlm.nih.gov/pubmed/35160720
http://dx.doi.org/10.3390/ma15030775
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author Cascione, Mariafrancesca
Rizzello, Loris
Manno, Daniela
Serra, Antonio
De Matteis, Valeria
author_facet Cascione, Mariafrancesca
Rizzello, Loris
Manno, Daniela
Serra, Antonio
De Matteis, Valeria
author_sort Cascione, Mariafrancesca
collection PubMed
description The use of silver nanoparticles (Ag NPs) in the biomedical field deserves a mindful analysis of the possible inflammatory response which could limit their use in the clinic. Despite the anti-cancer properties of Ag NPs having been widely demonstrated, there are still few studies concerning their involvement in the activation of specific inflammatory pathways. The inflammatory outcome depends on the synthetic route used in the NPs production, in which toxic reagents are employed. In this work, we compared two types of Ag NPs, obtained by two different chemical routes: conventional synthesis using sodium citrate and a green protocol based on leaf extracts as a source of reduction and capping agents. A careful physicochemical characterization was carried out showing spherical and stable Ag NPs with an average size between 20 nm and 35 nm for conventional and green Ag NPs respectively. Then, we evaluated their ability to induce the activation of inflammation in Human Leukemic Monocytes (THP-1) differentiated into M0 macrophages using 1 µM and 2 µM NPs concentrations (corresponded to 0.1 µg/mL and 0.2 µg/mL respectively) and two-time points (24 h and 48 h). Our results showed a clear difference in Nuclear Factor κB (NF-κb) activation, Interleukins 6–8 (IL-6, IL-8) secretion, Tumor Necrosis Factor-α (TNF-α) and Cyclooxygenase-2 (COX-2) expression exerted by the two kinds of Ag NPs. Green Ag NPs were definitely tolerated by macrophages compared to conventional Ag NPs which induced the activation of all the factors mentioned above. Subsequently, the exposure of breast cancer cell line (MCF-7) to the green Ag NPs showed that they exhibited antitumor activity like the conventional ones, but surprisingly, using the MCF-10A line (not tumoral breast cells) the green Ag NPs did not cause a significant decrease in cell viability.
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spelling pubmed-88365032022-02-12 Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes Cascione, Mariafrancesca Rizzello, Loris Manno, Daniela Serra, Antonio De Matteis, Valeria Materials (Basel) Article The use of silver nanoparticles (Ag NPs) in the biomedical field deserves a mindful analysis of the possible inflammatory response which could limit their use in the clinic. Despite the anti-cancer properties of Ag NPs having been widely demonstrated, there are still few studies concerning their involvement in the activation of specific inflammatory pathways. The inflammatory outcome depends on the synthetic route used in the NPs production, in which toxic reagents are employed. In this work, we compared two types of Ag NPs, obtained by two different chemical routes: conventional synthesis using sodium citrate and a green protocol based on leaf extracts as a source of reduction and capping agents. A careful physicochemical characterization was carried out showing spherical and stable Ag NPs with an average size between 20 nm and 35 nm for conventional and green Ag NPs respectively. Then, we evaluated their ability to induce the activation of inflammation in Human Leukemic Monocytes (THP-1) differentiated into M0 macrophages using 1 µM and 2 µM NPs concentrations (corresponded to 0.1 µg/mL and 0.2 µg/mL respectively) and two-time points (24 h and 48 h). Our results showed a clear difference in Nuclear Factor κB (NF-κb) activation, Interleukins 6–8 (IL-6, IL-8) secretion, Tumor Necrosis Factor-α (TNF-α) and Cyclooxygenase-2 (COX-2) expression exerted by the two kinds of Ag NPs. Green Ag NPs were definitely tolerated by macrophages compared to conventional Ag NPs which induced the activation of all the factors mentioned above. Subsequently, the exposure of breast cancer cell line (MCF-7) to the green Ag NPs showed that they exhibited antitumor activity like the conventional ones, but surprisingly, using the MCF-10A line (not tumoral breast cells) the green Ag NPs did not cause a significant decrease in cell viability. MDPI 2022-01-20 /pmc/articles/PMC8836503/ /pubmed/35160720 http://dx.doi.org/10.3390/ma15030775 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cascione, Mariafrancesca
Rizzello, Loris
Manno, Daniela
Serra, Antonio
De Matteis, Valeria
Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes
title Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes
title_full Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes
title_fullStr Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes
title_full_unstemmed Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes
title_short Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes
title_sort green silver nanoparticles promote inflammation shutdown in human leukemic monocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836503/
https://www.ncbi.nlm.nih.gov/pubmed/35160720
http://dx.doi.org/10.3390/ma15030775
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