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Identifying Predictive Bacterial Markers from Cervical Swab Microbiota on Pregnancy Outcome in Woman Undergoing Assisted Reproductive Technologies

Background and aims: Failure of the embryo to implant causes about three-fourths of lost pregnancies. Female genital tract microbiota has been associated to Assisted Reproductive Technologies (ART) outcomes. The objective of this study was to analyze the microbiota of human cervical swab and to corr...

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Autores principales: Villani, Annacandida, Fontana, Andrea, Barone, Stefano, de Stefani, Silvia, Primiterra, Mariangela, Copetti, Massimiliano, Panebianco, Concetta, Parri, Cristiana, Sciannamè, Natale, Quitadamo, Pasqua Anna, Tiezzi, Alessandra, Santana, Liliana, Maglione, Annamaria, D’Amato, Federica, Perri, Francesco, Palini, Simone, Pazienza, Valerio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836651/
https://www.ncbi.nlm.nih.gov/pubmed/35160131
http://dx.doi.org/10.3390/jcm11030680
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author Villani, Annacandida
Fontana, Andrea
Barone, Stefano
de Stefani, Silvia
Primiterra, Mariangela
Copetti, Massimiliano
Panebianco, Concetta
Parri, Cristiana
Sciannamè, Natale
Quitadamo, Pasqua Anna
Tiezzi, Alessandra
Santana, Liliana
Maglione, Annamaria
D’Amato, Federica
Perri, Francesco
Palini, Simone
Pazienza, Valerio
author_facet Villani, Annacandida
Fontana, Andrea
Barone, Stefano
de Stefani, Silvia
Primiterra, Mariangela
Copetti, Massimiliano
Panebianco, Concetta
Parri, Cristiana
Sciannamè, Natale
Quitadamo, Pasqua Anna
Tiezzi, Alessandra
Santana, Liliana
Maglione, Annamaria
D’Amato, Federica
Perri, Francesco
Palini, Simone
Pazienza, Valerio
author_sort Villani, Annacandida
collection PubMed
description Background and aims: Failure of the embryo to implant causes about three-fourths of lost pregnancies. Female genital tract microbiota has been associated to Assisted Reproductive Technologies (ART) outcomes. The objective of this study was to analyze the microbiota of human cervical swab and to correlate these findings with the ART outcomes. Materials and Methods: In this study, 88 cervical swabs were collected from women undergoing ART cycles, with various causes of infertility, at the beginning of the ART protocols. After microbial DNA extraction, V3–V4 variable regions of the 16S rRNA gene were amplified and sequenced on the Illumina MiSeq platform. PEnalized LOgistic Regression Analysis (PELORA) was performed to identify clusters of bacterial populations with differential abundances between patients with unfavorable and favorable pregnancy outcome groups, respectively. Results: We identified a core of microorganisms at lower taxonomic levels that were predictive of women’s pregnancy outcomes. Statistically significant differences were identified at species levels with Lactobacillus salivarius, Lactobacillus rhamnosus among others. Moreover the abundance of Lactobacillus crispatus and iners, respectively increased and decreased in favorable group as compared to unfavorable group, resulted within the core of microorganisms associated to positive ART outcome. Although the predominance of lactobacilli is generally considered to be advantageous for ART outcome, we found that also the presence of Bifidobacterium (together with the other lactobacilli) was more abundant in the favorable group. Discussion: Cervix is colonized by microorganisms which can play a role in ART outcomes as seen by an overall decrease in embryo attachment rates and pregnancy rates in both fertile and infertile women. If confirmed in a larger cohort, the abundance of these bacteria can be useful not only as a marker of unfavorable pregnancy outcome but also they may open the way to new interventional strategies based on genital tract microbiota manipulation in order to increase the pregnancy rates in woman undergoing assisted reproductive technologies.
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spelling pubmed-88366512022-02-12 Identifying Predictive Bacterial Markers from Cervical Swab Microbiota on Pregnancy Outcome in Woman Undergoing Assisted Reproductive Technologies Villani, Annacandida Fontana, Andrea Barone, Stefano de Stefani, Silvia Primiterra, Mariangela Copetti, Massimiliano Panebianco, Concetta Parri, Cristiana Sciannamè, Natale Quitadamo, Pasqua Anna Tiezzi, Alessandra Santana, Liliana Maglione, Annamaria D’Amato, Federica Perri, Francesco Palini, Simone Pazienza, Valerio J Clin Med Article Background and aims: Failure of the embryo to implant causes about three-fourths of lost pregnancies. Female genital tract microbiota has been associated to Assisted Reproductive Technologies (ART) outcomes. The objective of this study was to analyze the microbiota of human cervical swab and to correlate these findings with the ART outcomes. Materials and Methods: In this study, 88 cervical swabs were collected from women undergoing ART cycles, with various causes of infertility, at the beginning of the ART protocols. After microbial DNA extraction, V3–V4 variable regions of the 16S rRNA gene were amplified and sequenced on the Illumina MiSeq platform. PEnalized LOgistic Regression Analysis (PELORA) was performed to identify clusters of bacterial populations with differential abundances between patients with unfavorable and favorable pregnancy outcome groups, respectively. Results: We identified a core of microorganisms at lower taxonomic levels that were predictive of women’s pregnancy outcomes. Statistically significant differences were identified at species levels with Lactobacillus salivarius, Lactobacillus rhamnosus among others. Moreover the abundance of Lactobacillus crispatus and iners, respectively increased and decreased in favorable group as compared to unfavorable group, resulted within the core of microorganisms associated to positive ART outcome. Although the predominance of lactobacilli is generally considered to be advantageous for ART outcome, we found that also the presence of Bifidobacterium (together with the other lactobacilli) was more abundant in the favorable group. Discussion: Cervix is colonized by microorganisms which can play a role in ART outcomes as seen by an overall decrease in embryo attachment rates and pregnancy rates in both fertile and infertile women. If confirmed in a larger cohort, the abundance of these bacteria can be useful not only as a marker of unfavorable pregnancy outcome but also they may open the way to new interventional strategies based on genital tract microbiota manipulation in order to increase the pregnancy rates in woman undergoing assisted reproductive technologies. MDPI 2022-01-28 /pmc/articles/PMC8836651/ /pubmed/35160131 http://dx.doi.org/10.3390/jcm11030680 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Villani, Annacandida
Fontana, Andrea
Barone, Stefano
de Stefani, Silvia
Primiterra, Mariangela
Copetti, Massimiliano
Panebianco, Concetta
Parri, Cristiana
Sciannamè, Natale
Quitadamo, Pasqua Anna
Tiezzi, Alessandra
Santana, Liliana
Maglione, Annamaria
D’Amato, Federica
Perri, Francesco
Palini, Simone
Pazienza, Valerio
Identifying Predictive Bacterial Markers from Cervical Swab Microbiota on Pregnancy Outcome in Woman Undergoing Assisted Reproductive Technologies
title Identifying Predictive Bacterial Markers from Cervical Swab Microbiota on Pregnancy Outcome in Woman Undergoing Assisted Reproductive Technologies
title_full Identifying Predictive Bacterial Markers from Cervical Swab Microbiota on Pregnancy Outcome in Woman Undergoing Assisted Reproductive Technologies
title_fullStr Identifying Predictive Bacterial Markers from Cervical Swab Microbiota on Pregnancy Outcome in Woman Undergoing Assisted Reproductive Technologies
title_full_unstemmed Identifying Predictive Bacterial Markers from Cervical Swab Microbiota on Pregnancy Outcome in Woman Undergoing Assisted Reproductive Technologies
title_short Identifying Predictive Bacterial Markers from Cervical Swab Microbiota on Pregnancy Outcome in Woman Undergoing Assisted Reproductive Technologies
title_sort identifying predictive bacterial markers from cervical swab microbiota on pregnancy outcome in woman undergoing assisted reproductive technologies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836651/
https://www.ncbi.nlm.nih.gov/pubmed/35160131
http://dx.doi.org/10.3390/jcm11030680
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