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The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin

The search for new microbicide compounds is of an urgent need, especially against difficult-to-eradicate biofilm-forming bacteria. One attractive option is the application of cationic multivalent dendrimers as antibacterials and also as carriers of active molecules. These compounds require an adequa...

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Autores principales: Quintana-Sanchez, Sara, Gómez-Casanova, Natalia, Sánchez-Nieves, Javier, Gómez, Rafael, Rachuna, Jarosław, Wąsik, Sławomir, Semaniak, Jacek, Maciejewska, Barbara, Drulis-Kawa, Zuzanna, Ciepluch, Karol, de la Mata, F. Javier, Arabski, Michał
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836974/
https://www.ncbi.nlm.nih.gov/pubmed/35163794
http://dx.doi.org/10.3390/ijms23031873
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author Quintana-Sanchez, Sara
Gómez-Casanova, Natalia
Sánchez-Nieves, Javier
Gómez, Rafael
Rachuna, Jarosław
Wąsik, Sławomir
Semaniak, Jacek
Maciejewska, Barbara
Drulis-Kawa, Zuzanna
Ciepluch, Karol
de la Mata, F. Javier
Arabski, Michał
author_facet Quintana-Sanchez, Sara
Gómez-Casanova, Natalia
Sánchez-Nieves, Javier
Gómez, Rafael
Rachuna, Jarosław
Wąsik, Sławomir
Semaniak, Jacek
Maciejewska, Barbara
Drulis-Kawa, Zuzanna
Ciepluch, Karol
de la Mata, F. Javier
Arabski, Michał
author_sort Quintana-Sanchez, Sara
collection PubMed
description The search for new microbicide compounds is of an urgent need, especially against difficult-to-eradicate biofilm-forming bacteria. One attractive option is the application of cationic multivalent dendrimers as antibacterials and also as carriers of active molecules. These compounds require an adequate hydrophilic/hydrophobic structural balance to maximize the effect. Herein, we evaluated the antimicrobial activity of cationic carbosilane (CBS) dendrimers unmodified or modified with polyethylene glycol (PEG) units, against planktonic and biofilm-forming P. aeruginosa culture. Our study revealed that the presence of PEG destabilized the hydrophilic/hydrophobic balance but reduced the antibacterial activity measured by microbiological cultivation methods, laser interferometry and fluorescence microscopy. On the other hand, the activity can be improved by the combination of the CBS dendrimers with endolysin, a bacteriophage-encoded peptidoglycan hydrolase. This enzyme applied in the absence of the cationic CBS dendrimers is ineffective against Gram-negative bacteria because of the protective outer membrane shield. However, the endolysin—CBS dendrimer mixture enables the penetration through the membrane and then deterioration of the peptidoglycan layer, providing a synergic antimicrobial effect.
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spelling pubmed-88369742022-02-12 The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin Quintana-Sanchez, Sara Gómez-Casanova, Natalia Sánchez-Nieves, Javier Gómez, Rafael Rachuna, Jarosław Wąsik, Sławomir Semaniak, Jacek Maciejewska, Barbara Drulis-Kawa, Zuzanna Ciepluch, Karol de la Mata, F. Javier Arabski, Michał Int J Mol Sci Article The search for new microbicide compounds is of an urgent need, especially against difficult-to-eradicate biofilm-forming bacteria. One attractive option is the application of cationic multivalent dendrimers as antibacterials and also as carriers of active molecules. These compounds require an adequate hydrophilic/hydrophobic structural balance to maximize the effect. Herein, we evaluated the antimicrobial activity of cationic carbosilane (CBS) dendrimers unmodified or modified with polyethylene glycol (PEG) units, against planktonic and biofilm-forming P. aeruginosa culture. Our study revealed that the presence of PEG destabilized the hydrophilic/hydrophobic balance but reduced the antibacterial activity measured by microbiological cultivation methods, laser interferometry and fluorescence microscopy. On the other hand, the activity can be improved by the combination of the CBS dendrimers with endolysin, a bacteriophage-encoded peptidoglycan hydrolase. This enzyme applied in the absence of the cationic CBS dendrimers is ineffective against Gram-negative bacteria because of the protective outer membrane shield. However, the endolysin—CBS dendrimer mixture enables the penetration through the membrane and then deterioration of the peptidoglycan layer, providing a synergic antimicrobial effect. MDPI 2022-02-07 /pmc/articles/PMC8836974/ /pubmed/35163794 http://dx.doi.org/10.3390/ijms23031873 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Quintana-Sanchez, Sara
Gómez-Casanova, Natalia
Sánchez-Nieves, Javier
Gómez, Rafael
Rachuna, Jarosław
Wąsik, Sławomir
Semaniak, Jacek
Maciejewska, Barbara
Drulis-Kawa, Zuzanna
Ciepluch, Karol
de la Mata, F. Javier
Arabski, Michał
The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
title The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
title_full The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
title_fullStr The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
title_full_unstemmed The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
title_short The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin
title_sort antibacterial effect of pegylated carbosilane dendrimers on p. aeruginosa alone and in combination with phage-derived endolysin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836974/
https://www.ncbi.nlm.nih.gov/pubmed/35163794
http://dx.doi.org/10.3390/ijms23031873
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