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Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration

In the last decades, cell-based approaches for bone tissue engineering (BTE) have relied on using models that cannot replicate the complexity of the bone microenvironment. There is an ongoing amount of research on scaffold development responding to the need for feasible materials that can mimic the...

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Autores principales: Laboy-López, Simara, Méndez Fernández, Pedro O., Padilla-Zayas, Jorge G., Nicolau, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837436/
https://www.ncbi.nlm.nih.gov/pubmed/35154326
http://dx.doi.org/10.1155/2022/3255039
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author Laboy-López, Simara
Méndez Fernández, Pedro O.
Padilla-Zayas, Jorge G.
Nicolau, Eduardo
author_facet Laboy-López, Simara
Méndez Fernández, Pedro O.
Padilla-Zayas, Jorge G.
Nicolau, Eduardo
author_sort Laboy-López, Simara
collection PubMed
description In the last decades, cell-based approaches for bone tissue engineering (BTE) have relied on using models that cannot replicate the complexity of the bone microenvironment. There is an ongoing amount of research on scaffold development responding to the need for feasible materials that can mimic the bone extracellular matrix (ECM) and aid bone tissue regeneration (BTR). In this work, a porous cellulose acetate (CA) fiber mat was developed using the electrospinning technique and the mats were chemically modified to bioactivate their surface and promote osteoconduction and osteoinduction. The mats were characterized using FTIR and SEM/EDS to validate the chemical modifications and assess their structural integrity. By coupling adhesive peptides KRSR, RGD, and growth factor BMP-2, the fiber mats were bioactivated, and their induced biological responses were evaluated by employing immunocytochemical (ICC) techniques to study the adhesion, proliferation, and differentiation of premature osteoblast cells (hFOB 1.19). The biological assessment revealed that at short culturing periods of 48 hours and 7 days, the presence of the peptides was significant for proliferation and adhesion, whereas at longer culture times of 14 days, it had no significant effect on differentiation and maturation of the osteogenic progenitor cells. Based on the obtained results, it is thus concluded that the CA porous fiber mats provide a promising surface morphology that is both biocompatible and can be rendered bioactive upon the addition of osteogenic peptides to favor osteoconduction leading to new tissue formation.
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spelling pubmed-88374362022-02-12 Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration Laboy-López, Simara Méndez Fernández, Pedro O. Padilla-Zayas, Jorge G. Nicolau, Eduardo Int J Biomater Research Article In the last decades, cell-based approaches for bone tissue engineering (BTE) have relied on using models that cannot replicate the complexity of the bone microenvironment. There is an ongoing amount of research on scaffold development responding to the need for feasible materials that can mimic the bone extracellular matrix (ECM) and aid bone tissue regeneration (BTR). In this work, a porous cellulose acetate (CA) fiber mat was developed using the electrospinning technique and the mats were chemically modified to bioactivate their surface and promote osteoconduction and osteoinduction. The mats were characterized using FTIR and SEM/EDS to validate the chemical modifications and assess their structural integrity. By coupling adhesive peptides KRSR, RGD, and growth factor BMP-2, the fiber mats were bioactivated, and their induced biological responses were evaluated by employing immunocytochemical (ICC) techniques to study the adhesion, proliferation, and differentiation of premature osteoblast cells (hFOB 1.19). The biological assessment revealed that at short culturing periods of 48 hours and 7 days, the presence of the peptides was significant for proliferation and adhesion, whereas at longer culture times of 14 days, it had no significant effect on differentiation and maturation of the osteogenic progenitor cells. Based on the obtained results, it is thus concluded that the CA porous fiber mats provide a promising surface morphology that is both biocompatible and can be rendered bioactive upon the addition of osteogenic peptides to favor osteoconduction leading to new tissue formation. Hindawi 2022-02-04 /pmc/articles/PMC8837436/ /pubmed/35154326 http://dx.doi.org/10.1155/2022/3255039 Text en Copyright © 2022 Simara Laboy-López et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Laboy-López, Simara
Méndez Fernández, Pedro O.
Padilla-Zayas, Jorge G.
Nicolau, Eduardo
Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration
title Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration
title_full Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration
title_fullStr Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration
title_full_unstemmed Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration
title_short Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration
title_sort bioactive cellulose acetate electrospun mats as scaffolds for bone tissue regeneration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837436/
https://www.ncbi.nlm.nih.gov/pubmed/35154326
http://dx.doi.org/10.1155/2022/3255039
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