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Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration
In the last decades, cell-based approaches for bone tissue engineering (BTE) have relied on using models that cannot replicate the complexity of the bone microenvironment. There is an ongoing amount of research on scaffold development responding to the need for feasible materials that can mimic the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837436/ https://www.ncbi.nlm.nih.gov/pubmed/35154326 http://dx.doi.org/10.1155/2022/3255039 |
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author | Laboy-López, Simara Méndez Fernández, Pedro O. Padilla-Zayas, Jorge G. Nicolau, Eduardo |
author_facet | Laboy-López, Simara Méndez Fernández, Pedro O. Padilla-Zayas, Jorge G. Nicolau, Eduardo |
author_sort | Laboy-López, Simara |
collection | PubMed |
description | In the last decades, cell-based approaches for bone tissue engineering (BTE) have relied on using models that cannot replicate the complexity of the bone microenvironment. There is an ongoing amount of research on scaffold development responding to the need for feasible materials that can mimic the bone extracellular matrix (ECM) and aid bone tissue regeneration (BTR). In this work, a porous cellulose acetate (CA) fiber mat was developed using the electrospinning technique and the mats were chemically modified to bioactivate their surface and promote osteoconduction and osteoinduction. The mats were characterized using FTIR and SEM/EDS to validate the chemical modifications and assess their structural integrity. By coupling adhesive peptides KRSR, RGD, and growth factor BMP-2, the fiber mats were bioactivated, and their induced biological responses were evaluated by employing immunocytochemical (ICC) techniques to study the adhesion, proliferation, and differentiation of premature osteoblast cells (hFOB 1.19). The biological assessment revealed that at short culturing periods of 48 hours and 7 days, the presence of the peptides was significant for proliferation and adhesion, whereas at longer culture times of 14 days, it had no significant effect on differentiation and maturation of the osteogenic progenitor cells. Based on the obtained results, it is thus concluded that the CA porous fiber mats provide a promising surface morphology that is both biocompatible and can be rendered bioactive upon the addition of osteogenic peptides to favor osteoconduction leading to new tissue formation. |
format | Online Article Text |
id | pubmed-8837436 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-88374362022-02-12 Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration Laboy-López, Simara Méndez Fernández, Pedro O. Padilla-Zayas, Jorge G. Nicolau, Eduardo Int J Biomater Research Article In the last decades, cell-based approaches for bone tissue engineering (BTE) have relied on using models that cannot replicate the complexity of the bone microenvironment. There is an ongoing amount of research on scaffold development responding to the need for feasible materials that can mimic the bone extracellular matrix (ECM) and aid bone tissue regeneration (BTR). In this work, a porous cellulose acetate (CA) fiber mat was developed using the electrospinning technique and the mats were chemically modified to bioactivate their surface and promote osteoconduction and osteoinduction. The mats were characterized using FTIR and SEM/EDS to validate the chemical modifications and assess their structural integrity. By coupling adhesive peptides KRSR, RGD, and growth factor BMP-2, the fiber mats were bioactivated, and their induced biological responses were evaluated by employing immunocytochemical (ICC) techniques to study the adhesion, proliferation, and differentiation of premature osteoblast cells (hFOB 1.19). The biological assessment revealed that at short culturing periods of 48 hours and 7 days, the presence of the peptides was significant for proliferation and adhesion, whereas at longer culture times of 14 days, it had no significant effect on differentiation and maturation of the osteogenic progenitor cells. Based on the obtained results, it is thus concluded that the CA porous fiber mats provide a promising surface morphology that is both biocompatible and can be rendered bioactive upon the addition of osteogenic peptides to favor osteoconduction leading to new tissue formation. Hindawi 2022-02-04 /pmc/articles/PMC8837436/ /pubmed/35154326 http://dx.doi.org/10.1155/2022/3255039 Text en Copyright © 2022 Simara Laboy-López et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Laboy-López, Simara Méndez Fernández, Pedro O. Padilla-Zayas, Jorge G. Nicolau, Eduardo Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration |
title | Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration |
title_full | Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration |
title_fullStr | Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration |
title_full_unstemmed | Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration |
title_short | Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration |
title_sort | bioactive cellulose acetate electrospun mats as scaffolds for bone tissue regeneration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837436/ https://www.ncbi.nlm.nih.gov/pubmed/35154326 http://dx.doi.org/10.1155/2022/3255039 |
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