Cargando…
Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner
Maternal methyl donor supplementation during pregnancy has demonstrated lasting influence on offspring DNA methylation. However, it is unknown whether an adverse postnatal environment, such as high-fat (HF) feeding, overrides the influence of prenatal methyl donor supplementation on offspring epigen...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837519/ https://www.ncbi.nlm.nih.gov/pubmed/35165655 http://dx.doi.org/10.3389/fnut.2022.841787 |
_version_ | 1784649927459078144 |
---|---|
author | Korsmo, Hunter W. Dave, Bhoomi Trasino, Steven Saxena, Anjana Liu, Jia Caviglia, Jorge Matias Edwards, Kaydine Dembitzer, Moshe Sheeraz, Shameera Khaldi, Sarah Jiang, Xinyin |
author_facet | Korsmo, Hunter W. Dave, Bhoomi Trasino, Steven Saxena, Anjana Liu, Jia Caviglia, Jorge Matias Edwards, Kaydine Dembitzer, Moshe Sheeraz, Shameera Khaldi, Sarah Jiang, Xinyin |
author_sort | Korsmo, Hunter W. |
collection | PubMed |
description | Maternal methyl donor supplementation during pregnancy has demonstrated lasting influence on offspring DNA methylation. However, it is unknown whether an adverse postnatal environment, such as high-fat (HF) feeding, overrides the influence of prenatal methyl donor supplementation on offspring epigenome. In this study, we examined whether maternal supplementation of choline (CS), a methyl donor, interacts with prenatal and postnatal HF feeding to alter global and site-specific DNA methylation in offspring. We fed wild-type C57BL/6J mouse dams a HF diet with or without CS throughout gestation. After weaning, the offspring were exposed to HF feeding for 6 weeks resembling a continued obesogenic environment. Our results suggest that maternal CS under the HF condition (HFCS) increased global DNA methylation and DNA methyltransferase 1 (Dnmt1) expression in both fetal liver and brain. However, during the postnatal period, HFCS offspring demonstrated lower global DNA methylation and Dnmt1 expression was unaltered in both the liver and visceral adipose tissue. Site-specific DNA methylation analysis during both fetal and postnatal periods demonstrated that HFCS offspring had higher methylation of CpGs in the promoter of Srebf1, a key mediator of de novo lipogenesis. In conclusion, the influence of maternal CS on offspring DNA methylation is specific to HF feeding status during prenatal and postnatal periods. Without continued CS during the postnatal period, global DNA methylation enhanced by prenatal CS in the offspring was overridden by postnatal HF feeding. |
format | Online Article Text |
id | pubmed-8837519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88375192022-02-13 Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner Korsmo, Hunter W. Dave, Bhoomi Trasino, Steven Saxena, Anjana Liu, Jia Caviglia, Jorge Matias Edwards, Kaydine Dembitzer, Moshe Sheeraz, Shameera Khaldi, Sarah Jiang, Xinyin Front Nutr Nutrition Maternal methyl donor supplementation during pregnancy has demonstrated lasting influence on offspring DNA methylation. However, it is unknown whether an adverse postnatal environment, such as high-fat (HF) feeding, overrides the influence of prenatal methyl donor supplementation on offspring epigenome. In this study, we examined whether maternal supplementation of choline (CS), a methyl donor, interacts with prenatal and postnatal HF feeding to alter global and site-specific DNA methylation in offspring. We fed wild-type C57BL/6J mouse dams a HF diet with or without CS throughout gestation. After weaning, the offspring were exposed to HF feeding for 6 weeks resembling a continued obesogenic environment. Our results suggest that maternal CS under the HF condition (HFCS) increased global DNA methylation and DNA methyltransferase 1 (Dnmt1) expression in both fetal liver and brain. However, during the postnatal period, HFCS offspring demonstrated lower global DNA methylation and Dnmt1 expression was unaltered in both the liver and visceral adipose tissue. Site-specific DNA methylation analysis during both fetal and postnatal periods demonstrated that HFCS offspring had higher methylation of CpGs in the promoter of Srebf1, a key mediator of de novo lipogenesis. In conclusion, the influence of maternal CS on offspring DNA methylation is specific to HF feeding status during prenatal and postnatal periods. Without continued CS during the postnatal period, global DNA methylation enhanced by prenatal CS in the offspring was overridden by postnatal HF feeding. Frontiers Media S.A. 2022-01-28 /pmc/articles/PMC8837519/ /pubmed/35165655 http://dx.doi.org/10.3389/fnut.2022.841787 Text en Copyright © 2022 Korsmo, Dave, Trasino, Saxena, Liu, Caviglia, Edwards, Dembitzer, Sheeraz, Khaldi and Jiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Korsmo, Hunter W. Dave, Bhoomi Trasino, Steven Saxena, Anjana Liu, Jia Caviglia, Jorge Matias Edwards, Kaydine Dembitzer, Moshe Sheeraz, Shameera Khaldi, Sarah Jiang, Xinyin Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner |
title | Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner |
title_full | Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner |
title_fullStr | Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner |
title_full_unstemmed | Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner |
title_short | Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner |
title_sort | maternal choline supplementation and high-fat feeding interact to influence dna methylation in offspring in a time-specific manner |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837519/ https://www.ncbi.nlm.nih.gov/pubmed/35165655 http://dx.doi.org/10.3389/fnut.2022.841787 |
work_keys_str_mv | AT korsmohunterw maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner AT davebhoomi maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner AT trasinosteven maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner AT saxenaanjana maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner AT liujia maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner AT cavigliajorgematias maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner AT edwardskaydine maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner AT dembitzermoshe maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner AT sheerazshameera maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner AT khaldisarah maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner AT jiangxinyin maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner |