Cargando…

Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner

Maternal methyl donor supplementation during pregnancy has demonstrated lasting influence on offspring DNA methylation. However, it is unknown whether an adverse postnatal environment, such as high-fat (HF) feeding, overrides the influence of prenatal methyl donor supplementation on offspring epigen...

Descripción completa

Detalles Bibliográficos
Autores principales: Korsmo, Hunter W., Dave, Bhoomi, Trasino, Steven, Saxena, Anjana, Liu, Jia, Caviglia, Jorge Matias, Edwards, Kaydine, Dembitzer, Moshe, Sheeraz, Shameera, Khaldi, Sarah, Jiang, Xinyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837519/
https://www.ncbi.nlm.nih.gov/pubmed/35165655
http://dx.doi.org/10.3389/fnut.2022.841787
_version_ 1784649927459078144
author Korsmo, Hunter W.
Dave, Bhoomi
Trasino, Steven
Saxena, Anjana
Liu, Jia
Caviglia, Jorge Matias
Edwards, Kaydine
Dembitzer, Moshe
Sheeraz, Shameera
Khaldi, Sarah
Jiang, Xinyin
author_facet Korsmo, Hunter W.
Dave, Bhoomi
Trasino, Steven
Saxena, Anjana
Liu, Jia
Caviglia, Jorge Matias
Edwards, Kaydine
Dembitzer, Moshe
Sheeraz, Shameera
Khaldi, Sarah
Jiang, Xinyin
author_sort Korsmo, Hunter W.
collection PubMed
description Maternal methyl donor supplementation during pregnancy has demonstrated lasting influence on offspring DNA methylation. However, it is unknown whether an adverse postnatal environment, such as high-fat (HF) feeding, overrides the influence of prenatal methyl donor supplementation on offspring epigenome. In this study, we examined whether maternal supplementation of choline (CS), a methyl donor, interacts with prenatal and postnatal HF feeding to alter global and site-specific DNA methylation in offspring. We fed wild-type C57BL/6J mouse dams a HF diet with or without CS throughout gestation. After weaning, the offspring were exposed to HF feeding for 6 weeks resembling a continued obesogenic environment. Our results suggest that maternal CS under the HF condition (HFCS) increased global DNA methylation and DNA methyltransferase 1 (Dnmt1) expression in both fetal liver and brain. However, during the postnatal period, HFCS offspring demonstrated lower global DNA methylation and Dnmt1 expression was unaltered in both the liver and visceral adipose tissue. Site-specific DNA methylation analysis during both fetal and postnatal periods demonstrated that HFCS offspring had higher methylation of CpGs in the promoter of Srebf1, a key mediator of de novo lipogenesis. In conclusion, the influence of maternal CS on offspring DNA methylation is specific to HF feeding status during prenatal and postnatal periods. Without continued CS during the postnatal period, global DNA methylation enhanced by prenatal CS in the offspring was overridden by postnatal HF feeding.
format Online
Article
Text
id pubmed-8837519
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88375192022-02-13 Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner Korsmo, Hunter W. Dave, Bhoomi Trasino, Steven Saxena, Anjana Liu, Jia Caviglia, Jorge Matias Edwards, Kaydine Dembitzer, Moshe Sheeraz, Shameera Khaldi, Sarah Jiang, Xinyin Front Nutr Nutrition Maternal methyl donor supplementation during pregnancy has demonstrated lasting influence on offspring DNA methylation. However, it is unknown whether an adverse postnatal environment, such as high-fat (HF) feeding, overrides the influence of prenatal methyl donor supplementation on offspring epigenome. In this study, we examined whether maternal supplementation of choline (CS), a methyl donor, interacts with prenatal and postnatal HF feeding to alter global and site-specific DNA methylation in offspring. We fed wild-type C57BL/6J mouse dams a HF diet with or without CS throughout gestation. After weaning, the offspring were exposed to HF feeding for 6 weeks resembling a continued obesogenic environment. Our results suggest that maternal CS under the HF condition (HFCS) increased global DNA methylation and DNA methyltransferase 1 (Dnmt1) expression in both fetal liver and brain. However, during the postnatal period, HFCS offspring demonstrated lower global DNA methylation and Dnmt1 expression was unaltered in both the liver and visceral adipose tissue. Site-specific DNA methylation analysis during both fetal and postnatal periods demonstrated that HFCS offspring had higher methylation of CpGs in the promoter of Srebf1, a key mediator of de novo lipogenesis. In conclusion, the influence of maternal CS on offspring DNA methylation is specific to HF feeding status during prenatal and postnatal periods. Without continued CS during the postnatal period, global DNA methylation enhanced by prenatal CS in the offspring was overridden by postnatal HF feeding. Frontiers Media S.A. 2022-01-28 /pmc/articles/PMC8837519/ /pubmed/35165655 http://dx.doi.org/10.3389/fnut.2022.841787 Text en Copyright © 2022 Korsmo, Dave, Trasino, Saxena, Liu, Caviglia, Edwards, Dembitzer, Sheeraz, Khaldi and Jiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Korsmo, Hunter W.
Dave, Bhoomi
Trasino, Steven
Saxena, Anjana
Liu, Jia
Caviglia, Jorge Matias
Edwards, Kaydine
Dembitzer, Moshe
Sheeraz, Shameera
Khaldi, Sarah
Jiang, Xinyin
Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner
title Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner
title_full Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner
title_fullStr Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner
title_full_unstemmed Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner
title_short Maternal Choline Supplementation and High-Fat Feeding Interact to Influence DNA Methylation in Offspring in a Time-Specific Manner
title_sort maternal choline supplementation and high-fat feeding interact to influence dna methylation in offspring in a time-specific manner
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837519/
https://www.ncbi.nlm.nih.gov/pubmed/35165655
http://dx.doi.org/10.3389/fnut.2022.841787
work_keys_str_mv AT korsmohunterw maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner
AT davebhoomi maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner
AT trasinosteven maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner
AT saxenaanjana maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner
AT liujia maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner
AT cavigliajorgematias maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner
AT edwardskaydine maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner
AT dembitzermoshe maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner
AT sheerazshameera maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner
AT khaldisarah maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner
AT jiangxinyin maternalcholinesupplementationandhighfatfeedinginteracttoinfluencednamethylationinoffspringinatimespecificmanner